Allele Dmel\futsch^EP1419

General Information
Symbol	Dmel\futsch^EP1419	Species	D. melanogaster
Name		FlyBase ID	FBal0118233
Feature type	allele	Associated gene	Dmel\futsch

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Hummel et al., 2000, Roos et al., 2000, Gene Disruption Project members, 2001-, Benos et al., 2001, Zhang et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site X:1,293,932..1,293,932 (Hummel et al., 2000, Zhang et al., 2001, Roos et al., 2000, Gene Disruption Project members, 2001-, Benos et al., 2001, Lee et al., 2010, Gogel et al., 2006)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference The P{EP} insertion is upstream of the futsch coding region. (Hummel et al., 2000)
Caused by insertion	P{EP}futsch^EP1419 (Hummel et al., 2000, Zhang et al., 2001, Roos et al., 2000, Gene Disruption Project members, 2001-, Benos et al., 2001, Lee et al., 2010, Gogel et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective, with Scer\GAL4^elav-C155 (Roos et al., 2000, Zhang et al., 2001) neurophysiology defective, with Scer\GAL4^elav-C155 (Zhang et al., 2001)
Phenotype Manifest In
	axon, with Scer\GAL4^sim.PS (Hummel et al., 2000) bouton, with Scer\GAL4^elav-C155 (Roos et al., 2000, Zhang et al., 2001) neuromuscular junction, with Scer\GAL4^elav-C155 (Zhang et al., 2001) photoreceptor cell, with Scer\GAL4^elav-C155 (Zhang et al., 2001) synapse, with Scer\GAL4^elav-C155 (Zhang et al., 2001)
Detailed Description
	Statement Reference Expression of futsch[EP1419] pre-synaptically, under the control of Scer\GAL4[elav-C155] has no significant effect on synaptic mitochondria. (Lee et al., 2010) When expression is driven by Scer\GAL4^elav-C155 the phenotype is similar to that of Fmr1 loss of function mutants - neuromuscular junction overgrowth with increased synaptic area, branching and bouton number. Neurotransmission at the NMJ is elevated, and in the photoreceptor is depressed. (Zhang et al., 2001) When expression of futsch^EP1419 is driven by Scer\GAL4^sim.PS, changes in the morphology of individual growth cones is seen, and minor defects in the projection of individual axons are also seen. However these early axonal phenotypes do not lead to defects in the later formation of the central nervous system (CNS) axon pattern. (Hummel et al., 2000) When futsch^EP1419 is expressed under the control of Scer\GAL4^elav-C155, a slight reduction in average bouton size is seen (about 7.1μm², compared to 9.1μm² in wild-type). (Roos et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4⁴⁴, futsch^EP1419 has axon phenotype, enhanceable by Rnor\MAP2^Scer\UAS.cAa, Scer\GAL4⁴⁴ (Hummel et al., 2000)
Enhancer of
Statement Reference futsch^EP1419, Scer\GAL4⁴⁴ is an enhancer of axon phenotype of Rnor\MAP2^Scer\UAS.cAa, Scer\GAL4⁴⁴ (Hummel et al., 2000)
Suppressor of
Statement Reference futsch^EP1419, Scer\GAL4^elav-C155 is a suppressor of mitochondrion phenotype of Hsap\LRRK2^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^elav-C155 (Lee et al., 2010) futsch^EP1419, Scer\GAL4^elav-C155 is a suppressor of neuromuscular junction phenotype of Hsap\LRRK2^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^elav-C155 (Lee et al., 2010) futsch^EP1419, Scer\GAL4^elav-C155 is a suppressor of type I bouton phenotype of Hsap\LRRK2^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^elav-C155 (Lee et al., 2010)
NOT Suppressor of
Statement Reference futsch^EP1419, Scer\GAL4^Mhc.PW is a non-suppressor of neuromuscular junction phenotype of Hsap\LRRK2^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^Mhc.PW (Lee et al., 2010) futsch^EP1419, Scer\GAL4^Mhc.PW is a non-suppressor of type I bouton phenotype of Hsap\LRRK2^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^Mhc.PW (Lee et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Co-expression of futsch[EP1419] pre-synaptically rescues the synaptic overgrowth phenotype found upon expression of Hsap\LRRK2[Scer\UAS.T:Zzzz\FLAG] pre-synaptically under the control of Scer\GAL4[elav-C155]. However, synaptic defects caused by post-synaptic expression of Hsap\LRRK2[Scer\UAS.T:Zzzz\FLAG] are not suppressed when futsch[EP1419] is expressed post-synaptically (under the control of Scer\GAL4[Mhc.PW]). The abnormal microtubule phenotype seen upon Hsap\LRRK2[Scer\UAS.T:Zzzz\FLAG] presynaptic expression is rescued by a futsch[EP1419] expression (both under the control of Scer\GAL4[elav-C155]). Neuronal co-expression of futsch[EP1419] with Hsap\LRRK2[Scer\UAS.T:Zzzz\FLAG] rescues the mitochondrial distribution phenotype seen upon Hsap\LRRK2[Scer\UAS.T:Zzzz\FLAG] expression alone. (Lee et al., 2010) Expression of futsch^EP1419 and Rnor\MAP2^Scer\UAS.cAa driven by Scer\GAL4⁴⁴ results in frequent axonal swellings and misprojection phenotypes. In 1/5 of neuromeres sensory neurons project their axons in on one of the two commissures across the central nervous system (CNS) midline and fasciculate with their contralateral counterparts, which they never do in wild-type. (Hummel et al., 2000)
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	10751 P{EP}futsch^EP1419 w¹¹¹⁸
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	EP(1)1419 (Benos et al., 2001) futsch^EP1419 unnamed (Zhang et al., 2001)
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Lee et al., 2010, J. Neurosci. 30(50): 16959--16969 LRRK2 kinase regulates synaptic morphology through distinct substrates at the presynaptic and postsynaptic compartments of the Drosophila neuromuscular junction. [FBrf0212570] Gogel et al., 2006, Mol. Cell. Neurosci. 33(2): 188--199 The Drosophila microtubule associated protein Futsch is phosphorylated by Shaggy/Zeste-white 3 at an homologous GSK3beta phosphorylation site in MAP1B. [FBrf0194917] Benos et al., 2001, Genome Res. 11(5): 710--730 From First Base: the sequence of the tip of the X chromosome of Drosophila melanogaster, a comparison of two sequencing strategies. [FBrf0135823] Zhang et al., 2001, Cell 107(5): 591--603 Drosophila fragile X-related gene regulates the MAP1B homolog Futsch to control synaptic structure and function. [FBrf0141416] Hummel et al., 2000, Neuron 26(2): 357--370 Drosophila Futsch/22C10 is a MAP1B-like protein required for dendritic and axonal development. [FBrf0128497] Roos et al., 2000, Neuron 26(2): 371--382 Drosophila Futsch regulates synaptic microtubule organization and is necessary for synaptic growth. [FBrf0128626]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]

Allele Dmel\futschEP1419

Allele Dmel\futsch^EP1419