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General Information
Symbol
Dmel\Pka-R2EP2162
Species
D. melanogaster
Name
FlyBase ID
FBal0118394
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{EP} insertion 89bp upstream of the predicted transcription start site.

Insertion components
P{EP}Pka-R2EP2162
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Pka-R2EP2162 under the control of Scer\GAL4JW1 results in outgrowths and duplications of the costa region of the wing. Expression of Pka-R2EP2162 under the control of Scer\GAL4E132 results in duplications of the costa region of the wing.

Embryos show axon guidance defects in ISNb, SNa and the CNS. The majority of defects are manifest as an increase in repulsion, particularly in the CNS.

Expression of Pka-R2EP2162 under the control of Scer\GAL4ey.PH results in eyes that are slightly reduced in size. Flies expressing Pka-R2EP2162 under the control of Scer\GAL4hs.2sev have wild-type eyes. Flies expressing Pka-R2EP2162 under the control of Scer\GAL4en-e16E have wild-type wings.

43% of ovarioles from homozygous females show defects in follicle formation and maturation. The defects include multinucleate nurse cells, too many or too few nurse cells within a follicle, absence of interfollicular stalks and an abnormally large number of somatic epithelial cells at the posterior end of the follicle. Multinucleate cells have no internal ring canals and follicles with multinucleate cells seem to have fewer nurse cells than normal. Large follicles with double and triple the normal number of nurse cells are seen and often these follicles are not separated from the next follicle by an interfollicular stalk. Only approximately 42% of homozygous flies retain normal circadian rhythmicity in constant environmental conditions. Mutant flies entrain normally to light/dark conditions but the rhythmicity rapidly dampens through the following constant darkness conditions. In the 42% of flies showing rhythmicity, a normal period of approximately 23.7 hours is seen. Homozygous flies show a robust resistance to ethanol, with the time taken to immobilise 50% of flies in an ethanol sensitivity assay being approximately 6 minutes longer than that of wild-type flies. There are no significant differences in ethanol permeability or metabolism rates compared to wild type. The response of homozygous flies to the first dose of cocaine is much lower than that of wild-type flies; significantly higher doses of cocaine are required to elicit substantial initial responses compared to wild type. Subsequent doses of cocaine fail to elicit robust sensitisation, in contrast to wild-type flies which show prominent sensitisation, measured as an enhanced response to the second cocaine exposure.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of CycEScer\UAS.cLa suppresses the small eye phenotype caused by expression of Pka-R2EP2162 under the control of Scer\GAL4ey.PH.

Xenogenetic Interactions
Statement
Reference

Co-expression of Pka-R2EP2162 enhances the locomotor defects in flies induced by Scer\GAL4elav-C155-driven overexpression of Hsap\APPAβ1-42.Scer\UAS.cIa.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
PKA-RIIEP2162
Pka-R2EP2162
pka RIIEP(2)2162
pka-RIIEP(2)2162
Name Synonyms
Secondary FlyBase IDs
    References (9)