Allele Dmel\Pka-R2^EP2162

General Information
Symbol	Dmel\Pka-R2^EP2162	Species	D. melanogaster
Name		FlyBase ID	FBal0118394
Feature type	allele	Associated gene	Dmel\Pka-R2

Map ( GBrowse )	Untitled Document
Allele class	loss of function allele
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Terman and Kolodkin, 2004)
Mutagen	P-element activity (Park et al., 2000, Gene Disruption Project members, 2001-, Tseng and Hariharan, 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 2R:5,913,494..5,913,494 (Park et al., 2000, Collier et al., 2004, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-, Iijima-Ando et al., 2009)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{EP} insertion 89bp upstream of the predicted transcription start site. (Park et al., 2000)
Caused by insertion	P{EP}Pka-R2^EP2162 (Park et al., 2000, Collier et al., 2004, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-, Iijima-Ando et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	chemical resistant, with Scer\GAL4[-]: (Park et al., 2000) fertile, with Scer\GAL4[-]: (Park et al., 2000) locomotor rhythm defective, with Scer\GAL4[-]: (Park et al., 2000) neuroanatomy defective (Terman and Kolodkin, 2004) viable, with Scer\GAL4[-]: (Park et al., 2000) visible, with Scer\GAL4^ey.PH (Tseng and Hariharan, 2002) visible, with Scer\GAL4^JW1 (Collier et al., 2004) visible, with Scer\GAL4^upd-E132 (Collier et al., 2004)
Phenotype Manifest In
	abdominal intersegmental nerve, with Scer\GAL4[-]: (Terman and Kolodkin, 2004) abdominal segmental nerve, with Scer\GAL4[-]: (Terman and Kolodkin, 2004) costal cell, with Scer\GAL4^JW1 (Collier et al., 2004) costal cell, with Scer\GAL4^upd-E132 (Collier et al., 2004) eye, with Scer\GAL4^ey.PH (Tseng and Hariharan, 2002) follicle cell, with Scer\GAL4[-]: (Park et al., 2000) interfollicle cell, with Scer\GAL4[-]: (Park et al., 2000) nurse cell, with Scer\GAL4[-]: (Park et al., 2000) nurse cell \| ectopic, with Scer\GAL4[-]: (Park et al., 2000) ovariole, with Scer\GAL4[-]: (Park et al., 2000) presumptive embryonic/larval central nervous system, with Scer\GAL4[-]: (Terman and Kolodkin, 2004) ventral nerve cord, with Scer\GAL4[-]: (Terman and Kolodkin, 2004) wing, with Scer\GAL4^JW1 (Collier et al., 2004) wing, with Scer\GAL4^upd-E132 (Collier et al., 2004)
Detailed Description
	Statement Reference Expression of Pka-R2^EP2162 under the control of Scer\GAL4^JW1 results in outgrowths and duplications of the costa region of the wing. Expression of Pka-R2^EP2162 under the control of Scer\GAL4^E132 results in duplications of the costa region of the wing. (Collier et al., 2004) Embryos show axon guidance defects in ISNb, SNa and the CNS. The majority of defects are manifest as an increase in repulsion, particularly in the CNS. (Terman and Kolodkin, 2004) Expression of Pka-R2^EP2162 under the control of Scer\GAL4^ey.PH results in eyes that are slightly reduced in size. Flies expressing Pka-R2^EP2162 under the control of Scer\GAL4^hs.2sev have wild-type eyes. Flies expressing Pka-R2^EP2162 under the control of Scer\GAL4^en-e16E have wild-type wings. (Tseng and Hariharan, 2002) 43% of ovarioles from homozygous females show defects in follicle formation and maturation. The defects include multinucleate nurse cells, too many or too few nurse cells within a follicle, absence of interfollicular stalks and an abnormally large number of somatic epithelial cells at the posterior end of the follicle. Multinucleate cells have no internal ring canals and follicles with multinucleate cells seem to have fewer nurse cells than normal. Large follicles with double and triple the normal number of nurse cells are seen and often these follicles are not separated from the next follicle by an interfollicular stalk. Only approximately 42% of homozygous flies retain normal circadian rhythmicity in constant environmental conditions. Mutant flies entrain normally to light/dark conditions but the rhythmicity rapidly dampens through the following constant darkness conditions. In the 42% of flies showing rhythmicity, a normal period of approximately 23.7 hours is seen. Homozygous flies show a robust resistance to ethanol, with the time taken to immobilise 50% of flies in an ethanol sensitivity assay being approximately 6 minutes longer than that of wild-type flies. There are no significant differences in ethanol permeability or metabolism rates compared to wild type. The response of homozygous flies to the first dose of cocaine is much lower than that of wild-type flies; significantly higher doses of cocaine are required to elicit substantial initial responses compared to wild type. Subsequent doses of cocaine fail to elicit robust sensitisation, in contrast to wild-type flies which show prominent sensitisation, measured as an enhanced response to the second cocaine exposure. (Park et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Pka-R2^EP2162, Scer\GAL4^ey.PH has visible phenotype, suppressible by CycE^Scer\UAS.cLa, Scer\GAL4^ey.PH (Tseng and Hariharan, 2002)
Enhancer of
Statement Reference Pka-R2^EP2162, Scer\GAL4^elav-C155 is an enhancer of locomotor behavior defective phenotype of Hsap\APP^{Aβ1-42.Scer\UAS.cIa}, Scer\GAL4^elav-C155 (Iijima-Ando et al., 2009)
Phenotype Manifest In
Suppressed by
Statement Reference Pka-R2^EP2162, Scer\GAL4^ey.PH has eye phenotype, suppressible by CycE^Scer\UAS.cLa, Scer\GAL4^ey.PH (Tseng and Hariharan, 2002)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of CycE^Scer\UAS.cLa suppresses the small eye phenotype caused by expression of Pka-R2^EP2162 under the control of Scer\GAL4^ey.PH. (Tseng and Hariharan, 2002)
Xenogenetic Interactions
Statement Reference Co-expression of Pka-R2[EP2162] enhances the locomotor defects in flies induced by Scer\GAL4[elav-C155]-driven overexpression of Hsap\APP[Aβ1-42.Scer\UAS.cIa]. (Iijima-Ando et al., 2009)
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	17029 w¹¹¹⁸; P{EP}Pka-R2^EP2162
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	Pka-R2^EP2162 PKA-RII^EP2162 (Zhong and Wu, 2003) pka RII^EP(2)2162 (Terman and Kolodkin, 2004) pka-RII^EP(2)2162 (Park et al., 2000)
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Iijima-Ando et al., 2009, PLoS ONE 4(12): e8310 Mitochondrial mislocalization underlies Abeta42-induced neuronal dysfunction in a Drosophila model of Alzheimer's disease. [FBrf0209556] Collier et al., 2004, Genetics 167(2): 783--796 Drosophila Costal1 mutations are alleles of protein kinase A that modulate hedgehog signaling. [FBrf0179171] Terman and Kolodkin, 2004, Science 303(5661): 1204--1207 Nervy links protein kinase A to plexin-mediated semaphorin repulsion. [FBrf0175134] Tseng and Hariharan, 2002, Genetics 162(1): 229--243 An overexpression screen in Drosophila for genes that restrict growth or cell-cycle progression in the developing eye. [FBrf0152035] Park et al., 2000, J. Biol. Chem. 275(27): 20588--20596 Type II cAMP-dependent protein kinase-deficient Drosophila are viable but show developmental, circadian, and drug response phenotypes. [FBrf0128602]
Review	Stanewsky, 2003, J. Neurobiol. 54(1): 111--147 Genetic analysis of the circadian system in Drosophila melanogaster and mammals. [FBrf0155881]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]
Abstract	Zhong and Wu, 2003, A. Dros. Res. Conf. 44: 843C An improved electrophysiological paradigm in behaving tethered flies demonstrates opposite effects of Drosophila PKA-RI and PKA-RII mutations on habituation and dishabituation. [FBrf0155255]

Allele Dmel\Pka-R2EP2162

Allele Dmel\Pka-R2^EP2162