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General Information
Symbol
Dmel\EcRdsRNA.hs.PL
Species
D. melanogaster
Name
FlyBase ID
FBal0118550
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
hs-EcR-RNAi, hs-EcRi
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Two copies of a 1092bp portion of the EcR common region are arranged as a head-to-head repeat located downstream of the hsp70 promoter and upstream of the Act5C transcription termination and polyadenylation signals.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Third instar larvae collected about 18 hours before puparium formation and subjected to a single heat treatment at 37oC for 30 minutes (to induce expression of EcRdsRNA.hs.PL) followed by recovery at 25oC, all arrest development at the larval-prepupal transition, as stationary untanned elongated prepupae with everted anterior spiracles.

Heat-shock induced expression of EcRdsRNA.hs.PL results in a high degree of lethality. Two sequential heat treatments, at about 18 and 12 hours before puparium formation generates the most severe phenotype. 81% of these animals arrest development as stationary late third instar larvae. They also fail to shorten their body to form the characteristic shape of a prepupa and die several days later. About 13% attempt to pupariate but form long prepupae that occasionally fail to tan and dies 1-2 days later. 6% of animals die as pupae with defects in leg elongation. Dissection of prepupae reveals normal larval midguts in newly formed prepupae but the midguts fail to progress beyond the stage normally seen in prepupae that are 2 hours old. The midgut fails to contract and undergo cell death, resulting in persistence of the gastric caeca and proventriculus. In addition the adult midgut does not appear to form, and the salivary glands are still present 24 hours after puparium formation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

When reared without heat-shock induction, about 10% of animals expressing Scer\GAL4Aug21-driven grimScer\UAS.cNa in the presence of EcRdsRNA.hs.PL survive to pharate adult stage and less than half of those emerge as adults.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Snapback dsRNA has been synthesised from the template under the control of a heatshock promoter to study the consequence of dsRNA interference (RNAi) of the EcR gene.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
EcRds.hs.PL
EcRds.hs.cCa
EcRdsRNA.hs.PL
Name Synonyms
Secondary FlyBase IDs
    References (5)