FB2025_02 , released April 17, 2025
Allele: Dmel\hepAct.UAS
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General Information
Symbol
Dmel\hepAct.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0119240
Feature type
allele
Associated gene
Dmel\hep
Associated Insertion(s)
Carried in Construct
P{UAS-Hep.Act}
Also Known As
UAS-hepact, UAS-hepCA, hepACT, UAS-hep, UAS-hep.Act, UAShepact, UAS-Hep-act
Key Links
Transgenic product class
missense_variant
(Weber et al., 2000)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Weber et al., 2000)
Mutagen
in vitro construct
(Weber et al., 2000)
Progenitor genotype
Carried in construct
P{UAS-Hep.Act}
Cytology
Description

Expression of a hep mutant coding region is governed by UAS (carries the amino acid replacements S326D and T330D).

(Weber et al., 2000)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
hep
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  heart disease
      CEA
      (Karekar et al., 2021)
      model of  carcinoma
      CEA with BacA\p35UAS.cHa
      (Uhlirova and Bohmann, 2006)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  gastrointestinal system cancer
      modeled by Apc2g10, ApcQ8
      (Wang et al., 2013)
      exacerbates  colorectal cancer
      modeled by Ras85DV12.UAS
      (Bangi et al., 2012)
      ameliorates  carcinoma
      modeled by scrib1
      (Uhlirova et al., 2005)
      modeled by RafUAS.F179
      (Uhlirova et al., 2005)
      exacerbates  carcinoma
      modeled by Ras85DV12.UAS
      (Uhlirova and Bohmann, 2006)
      exacerbates  cancer
      modeled by scribHMS01490
      (Kumar and Srikrishna, 2025)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of hepAct.UAS under the control of Scer\GAL4sev.EP induces a rough eye phenotype.

      (Mlih and Karpac, 2022)

      Expression of hepAct.Scer\UAS in eye-antennal disc clones increases cell death.

      (Külshammer and Uhlirova, 2013)

      Expression of hepAct.Scer\UAS under the control of Scer\GAL4GMR.PF results in a reduction in eye size; eye size is 19% of controls in both females and males.

      (Pechkovsky et al., 2013)

      Expression of hepAct.Scer\UAS under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B rapidly induces gut hyperplasia and eventually kills the affected fly.

      (Bond and Foley, 2012)

      Mutant adults are more susceptible to sustained 37[o]C heat shock than wild-type flies.

      (Gonda et al., 2012)

      Expression of hepAct.Scer\UAS under the control of Scer\GAL4ptc-559.1 results in a dramatic increase in the number of intercalated cells and the formation of ectopic mixer cells at the segment boundaries during dorsal closure (in the abdominal segments of wild-type embryos at the end of dorsal closure, a mixer cell moves across the segment boundary from the anterior compartment to the posterior compartment and two cells from the ventral ectoderm intercalate into the leading edge, posterior to the mixer cell).

      (Gettings et al., 2010)

      Ectopic expression of hepAct.Scer\UAS driven by Scer\GAL469B in embryos leads to cuticles with openings in the anterior part and puckering in the dorsal part.

      (Rousset et al., 2010)

      hepAct.Scer\UAS-expressing clones in the larval gut epithelium or larval fat body show a preponderance of autolysosomes/autophagosomes compared to controls.

      (Wu et al., 2009)

      Expression of hepAct.Scer\UAS under the control of Scer\GAL4GMR.PF results in a rough eye phenotype.

      (Jones et al., 2008)

      In embryos that express hepAct.Scer\UAS under the control of Scer\GAL4arm.PS, cells in the ventral part of the maxillary segment are elongated, instead of round like wild-type cells.

      (Hueber et al., 2007)

      Expression of hepAct.Scer\UAS under the control of Scer\GAL4slbo.2.6 has no significant effect on posterior or dorsal migration of border follicle cells.

      (Mathieu et al., 2007)

      Expression of hepAct.Scer\UAS in the wing, under the control of Scer\GAL4Bx-MS1096 results in mild or undetectable phenotypic changes.

      Expression of hepAct.Scer\UAS in the embryonic head, under the control of Scer\GAL4hs.PB, does not induce any effects on brain size.

      (Lee et al., 2006)

      Expression of hepAct.Scer\UAS in the notum of the wing imaginal discs, under the regulation of Scer\GAL4ap-md544 does not result in abnormalities of thorax closure such as cleft formation.

      (Cha et al., 2005)

      hepAct.Scer\UAS; Scer\GAL4sev.EP adults have severely malformed (sunken) eyes. 1 day old hepAct.Scer\UAS; Scer\GAL4Ilp2.PR adults are significantly smaller than wild-type.

      (Wang et al., 2005)

      hepAct.Scer\UAS when driven by Scer\GAL4αTub84B.PL in clones in the eye, results in very small clones.

      (Brumby and Richardson, 2003)

      When expression is driven by Scer\GAL4hs.2sev the eyes are significantly reduced with a messy appearance in sections. This may be due to cell death induction. Tissue polarity disruptions are evident when early eye discs are examined.

      (Weber et al., 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of hepAct.Scer\UAS under the control of Scer\GAL4Act.PU suppresses the hyperplasia seen in Apc2g10 ApcQ8 mutant intestinal stem cell clones.

      (Wang et al., 2013)

      Co-expression of hepAct.Scer\UAS enhances the dissemination of hindgut cells seen in flies expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4byn-Gal4.

      (Bangi et al., 2012)

      Co-expression of PvrDN.Scer\UAS and hepAct.Scer\UAS in 3-5 day old adult flies, under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B results in ISC proliferation.

      (Bond and Foley, 2012)

      Co-expression of hepAct.Scer\UAS enhances the severity of the posterior migration defects seen in border follicle cells that are expressing PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6.

      The posterior migration defects seen in border follicle cells expressing Pvf1EPg11235 under the control of Scer\GAL4slbo.2.6 are slightly enhanced if the cells are also co-expressing hepAct.Scer\UAS.

      The border follicle cell migration defects that are seen in flies co-expressing both PvrDN.Scer\UAS and hepAct.Scer\UAS under the control of Scer\GAL4slbo.2.6 are partially suppressed by the co-expression of either kaydsRNA.Scer\UAS or kaybZip.Scer\UAS.

      The border follicle cell migration defects that are seen in flies co-expressing both PvrDN.Scer\UAS and hepAct.Scer\UAS under the control of Scer\GAL4slbo.2.6 are not strongly affected by the co-expression of either JradsRNA.cBa.Scer\UAS or JraJbz.Scer\UAS.

      The border follicle cell migration defects that are seen in flies co-expressing both PvrDN.Scer\UAS and hepAct.Scer\UAS under the control of Scer\GAL4slbo.2.6 are partially suppressed by the co-expression of thScer\UAS.cHa.

      (Mathieu et al., 2007)

      Simultaneous expression of hepAct.Scer\UAS with lkb1Scer\UAS.cLa (both under the control of Scer\GAL4Bx-MS1096 strongly enhances the lkb1Scer\UAS.cLa phenotype, resulting in severe disruption in the structure and size of the wing blades.

      Ectopic activation of JNK signaling by overexpression of hepAct.Scer\UAS (under the control of Scer\GAL4hs.PB) completely rescues the 'big brain' phenotype of lkb1X5 mutants and restores embryonic apoptosis.

      (Lee et al., 2006)

      Coexpression of hepAct.Scer\UAS and Ras85DV12.Scer\UAS, under the control of Scer\GAL4Act5C.PI, in eye/antennal imaginal disc clones results in migration of some disc cells to ectopic regions of the brain, although cell invasion is not efficient in this genotype.

      (Uhlirova and Bohmann, 2006)

      Co-expression of hepAct.Scer\UAS, with parkScer\UAS.T:Hsap\MYC, in the notum of the wing imaginal discs, under the regulation of Scer\GAL4ap-md544 suppresses the parkScer\UAS.T:Hsap\MYC phenotype of thoracic cleft formation resulting from abnormalities in JNK signalling during thorax closure.

      (Cha et al., 2005)

      The presence of a cbtEP2237E1 background mutation reduces embryonic lethality from 100 to 36.4% when hepAct.Scer\UAS is expressed under the control of Scer\GAL469B.

      The presence of a cbtEP2237E1 background mutation reduces embryonic lethality from 100 to 36.4% when hepAct.Scer\UAS is expressed under the control of Scer\GAL464B.

      (Munoz-Descalzo et al., 2005)

      Expression of hepAct.Scer\UAS in scribunspecified mutant clones under the control of Scer\GAL4Act5C.PI suppressed the scribunspecified phenotype to revert to an essentially wild-type appearance, without the marked lesions resulting from scrib-deficient tissue. Significantly, these phenotypically rescued eyes do not contain any clonal cells, suggesting that JNK activity (increased by expression of hep) counteracts the outgrowth of scribunspecified tissue or causes the removal of the mutant cells. These eyes are the same size as wild-type. Expression of hepAct.Scer\UAS in eye imaginal disc clones that express phlScer\UAS.F179 (both under the control of Scer\GAL4Act5C.PI) suppresses the aggressive tissue expansion, resulting in clones of this genotype remaining much smaller than phlScer\UAS.F179 (under the control of Scer\GAL4Act5C.PI) clones. hepAct.Scer\UAS expression can elevate the occurence of apoptosis among phlScer\UAS.F179 expressing cells (from 6.0% to 17.3%). However, a significant fraction of hepAct.Scer\UAS phlScer\UAS.F179 (both under the control of Scer\GAL4Act5C.PI) clones survive (6.4%). Expression of hepAct.Scer\UAS in eye imaginal disc clones that express phlScer\UAS.F179 (under the control of Scer\GAL4Act5C.PI) and scribunspecified results in adult eyes with massive overgrowth. This is also true of eye imaginal disc clones that express phlScer\UAS.F179 and hepAct.Scer\UAS (under the control of Scer\GAL4Act5C.PI). The heads are significantly bigger and the retina of these mutants are dramatically larger than that of a wild-type eye. In many cases, the retina is folded and bunched to accommodate the surfeit of tissue. These severely hyperplastic eye structures are well patterned and show a distinctive ommatidial organisation. The tumourous overgrowth is induced non-autonomously in the wild-type cells surrounding the hepAct.Scer\UAS phlScer\UAS.F179 (both under the control of Scer\GAL4Act5C.PI) and hepAct.Scer\UAS phlScer\UAS.F179 (both under the control of Scer\GAL4Act5C.PI) scribunspecified cells.

      (Uhlirova et al., 2005)

      Expression of hepAct.Scer\UAS in eye imaginal disc clones that express phlScer\UAS.F179 (both under the control of Scer\GAL4Act5C.PI) suppresses the aggressive tissue expansion, resulting in clones of this genotype remaining much smaller than phlScer\UAS.F179 clones (under the control of Scer\GAL4Act5C.PI). hepAct.Scer\UAS expression can elevate the occurence of apoptosis among phlScer\UAS.F179 expressing cells (from 6.0% to 17.3%). However, a significant fraction of hepAct.Scer\UAS phlScer\UAS.F179 (both under the control of Scer\GAL4Act5C.PI) clones survive (6.4%).

      Clones of eye imaginal discs that express phlScer\UAS.F179 autonomously (under the control of Scer\GAL4Act5C.PI) in scrib1 clones grow into massive and invasive tumours during larval stages, resulting in 80% of animals not pupating and dying as giant larvae.

      Expression of hepAct.Scer\UAS in eye imaginal disc clones that express phlScer\UAS.F179 (under the control of Scer\GAL4Act5C.PI) results in adult eyes with massive overgrowth. The heads are significantly bigger and the retina of these mutants are dramatically larger than that of a wild-type eye. In many cases, the retina is folded and bunched to accommodate the surfeit of tissue. These severely hyperplastic eye structures are well patterned and show a distinctive ommatidial organisation. The tumourous overgrowth is induced non-autonomously in the wild-type cells surrounding the clones. The same phenotype tumorogenic phnotype occurs in flies that express both phlScer\UAS.F179 and hepAct.Scer\UAS, under the control of Scer\GAL4Act5C.PI, in a scrib1 background.

      Expression of hepAct.Scer\UAS in scrib673 mutant clones under the control of Scer\GAL4Act5C.PI suppressed the scrib2 phenotype to revert to an essentially wild-type appearance, without the marked lesions resulting from scrib-deficient tissue. Significantly, these phenotypically rescued eyes do not contain any clonal cells, suggesting that JNK activity (increased by expression of hep) counteracts the outgrowth of scribunspecified tissue or causes the removal of the mutant cells. These eyes are the same size as wild-type.

      (Uhlirova et al., 2005)

      The severe eye malformations seen in hepAct.Scer\UAS; Scer\GAL4sev.EP flies are largely suppressed by foxo21/+: resulting flies have slightly rough eyes. The reduction in body weight seen in 1 day old hepAct.Scer\UAS; Scer\GAL4Ilp2.PR adults is partially supressed by foxo25/+.

      (Wang et al., 2005)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Zzzz\E4orf4Scer\UAS.cPa partially rescues the eye phenotypes seen when hepAct.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF at 18[o]C. Eye size is restored to 23% and 31% of controls in females and males respectively (from 19% in each).

      (Pechkovsky et al., 2013)

      Expression of Styp\AvrAm.Scer\UAS.T:Hsap\MYC does not suppress the small eye phenotype seen when hepAct.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF.

      (Jones et al., 2008)

      The border follicle cell migration defects that are seen in flies co-expressing both PvrDN.Scer\UAS and hepAct.Scer\UAS under the control of Scer\GAL4slbo.2.6 are not suppressed by the co-expression of BacA\p35Scer\UAS.cHa.

      (Mathieu et al., 2007)

      Coexpression of hepAct.Scer\UAS and BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4dpp.blk1, in wing imaginal discs results in the scattering of cells into the adjacent area of the disc from the Scer\GAL4dpp.blk1 expression domain. These cells display extensive actin reorganization and extend actin rich filopodia.

      (Uhlirova and Bohmann, 2006)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (3)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (6)
      Reported As
      Symbol Synonym
      HepAct
      (Weber et al., 2000)
      Hepact
      (Uhlirova and Bohmann, 2006)
      hepACT
      (Munoz-Descalzo et al., 2005)
      hepAct.Scer\UAS
      hepAct.UAS
      hepAct
      (Kockel et al., 2001)
      Name Synonyms
      Secondary FlyBase IDs
        References (58)