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General Information
Symbol
Dmel\fafEP381
Species
D. melanogaster
Name
FlyBase ID
FBal0119253
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
EP(3)0381, EP381
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
The P{EP} element is inserted within the first intron, upstream of the entire coding region.
P{EP} insertion at -572bp relative to the start of the transcription unit.
Insertion components
P{EP}fafEP381
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
fafEP381 overexpression mutants exhibit defects in the giant fiber-tergotrochanteral motorneuron synapse, such as axonal overgrowth.
Neural expression of fafEP381, under the control of Scer\GAL4elav-C155 leads to an increased number of synaptic boutons and branches and an increased synaptic span.
Expression of fafEP381 under the control of Scer\GAL4GMR.PF at room temperature results in smooth glassy eyes (due to fusion of ommatidia) that are also moderately reduced in size.
Expression of fafEP381 under the control of Scer\GAL4Tab2-201Y results in misguidance and mushroom body overgrowth phenotypes.
Flies expressing fafEP381 under the control of Scer\GAL4ap-md544 have ectopic bristles on the dorsal surface of the wing blade.
When fafEP381 is driven by Scer\GAL4elav-C155, a marked expansion in the size and complexity is seen in the synapses. There is an increase in both the number of synaptic boutons and in the synaptic span. There is also an increase in the number of synaptic branches. When the evoked excitatory junctional potentials (EJPs) of fafEP381, Scer\GAL4elav-C155 embryos are examined, a marked reduction is seen in their amplitudes. A large decrease in quantal content is also seen, and the frequency of spontaneous mEJPs is also reduced.
Mutant larvae expressing fafEP381 under the control of Scer\GAL4elav-C155 have ISN neuron pathfinding defects. Mutant larvae expressing fafEP381 under the control of Scer\GAL4elav-C155 have excessive or ectopic synapses. Mutant larvae expressing fafEP381 under the control of Scer\GAL4elav-C155 have reduced or abnormal synapses. They have abnormal ISN synaptic branches and a reduced number of boutons.
In the dorsal muscle region of mutant larvae expressing fafEP381 under the control of Scer\GAL4elav-C155, the synapses at the second branchpoint and terminal arbor have abnormal structures, with a spray of extra branches emanating from the terminal arbor.
Expression of fafEP381 under the control of Scer\GAL4sca-537.4 results in loss of external support cells of the scutellar and dorsocentral macrochaetae without loss of the sheath cell. Shafts with a branched tip are seen. On the abdomen, dot-like shaft morphology is seen.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference
NOT suppressed by
Statement
Reference
Suppressor of
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Suppressed by
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference
Animals derived from homozygous fafEP381 females mated to dpphr4/+ males show strong lethality (only 26% of the expected number survive). The embryos have a ventralised cuticle phenotype.
wnd1/wnd2 mutants suppress the synapse phenotype of fafEP381, under the control of Scer\GAL4elav-C155.
The fafEP381 (Scer\GAL4GMR.PF) overexpression phenotype is strongly enhanced by co-expression with pblEP3415 as the ommatidial field is reduced to approximately 30% of normal in Scer\GAL4GMR.PF-driven pblEP3415 and fafEP381 flies.
pnrMD237/+ adults expressing fafEP381 under the control of Scer\GAL4pnr-MD237 have a slight cleft in the abdominal dorsal midline and a reduced scutellum.
Xenogenetic Interactions
Statement
Reference
The synergistic effect of pblEP3415 and fafEP381 can be overcome by co-expression with Hsap\UBE3AScer\UAS.cRa (all under the control of Scer\GAL4GMR.PF. Co-expression of Hsap\UBE3AScer\UAS.cRa with fafEP381 (both under the control of Scer\GAL4GMR.PF) partially suppresses the smooth eye phenotype induced by expression of fafEP381 alone.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Selected as: a P{EP} insertion line that modifies the pnrMD237/+ phenotype when expressed using Scer\GAL4pnr-MD237.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (12)