FlyBase Allele Report: Dmel\Rab11[EP3017]

General Information

Symbol

Dmel\Rab11^EP3017

Species

D. melanogaster

Name

FlyBase ID

FBal0119426

Feature type

allele

Associated gene

Associated Insertion(s)

P{EP}Rab11^EP3017

Carried in Construct

Genomic Maps

GBrowse JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Sasikumar and Roy, 2009)

Mutagen

P-element activity

(Gene Disruption Project members, 2001-, Jankovics et al., 2001, Abdelilah-Seyfried et al., 2000)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

transposable element insertion site

3R:21,111,846..21,111,846 [+]

(Warner and Longmore, 2009, Gene Disruption Project members, 2001-, Jankovics et al., 2001, Abdelilah-Seyfried et al., 2000)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

P{EP} insertion at +222bp of the transcription unit (the CDS starts at +189bp).

(Abdelilah-Seyfried et al., 2000)

Insertion components

P{EP}Rab11^EP3017

Product class / Tool use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

misexpression element

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

cell lethal | recessive | somatic clone, with Scer\GAL4^- :

(Satoh et al., 2005)

cytokinesis defective | male (with Rab11^93Bi)

(Giansanti et al., 2007)

female sterile (with Rab11^93Bi), with Scer\GAL4^- :

(Jankovics et al., 2001)

(Giansanti et al., 2007)

lethal (with Df(3R)e-R1)

(Giansanti et al., 2007)

lethal (with Rab11^ETo3)

(Giansanti et al., 2007)

lethal (with Rab11^ETo11)

(Giansanti et al., 2007)

lethal | recessive, with Scer\GAL4^- :

(Jankovics et al., 2001)

lethal - all die before end of first instar larval stage | recessive

(Alone et al., 2005)

lethal - all die before end of larval stage (with Rab11^mo)

(Alone et al., 2005)

meiotic cell cycle defective | male (with Rab11^93Bi)

(Giansanti et al., 2007)

partially lethal - majority die (with Rab11^93Bi)

(Giansanti et al., 2007)

some die during first instar larval stage | recessive

(Alone et al., 2005)

some die during larval stage (with Rab11^mo)

(Alone et al., 2005)

visible, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

visible | somatic clone

(Alone et al., 2005)

Phenotype Manifest In

(Sasikumar and Roy, 2009)

amnioserosa (with Df(3R)e-R1)

(Sasikumar and Roy, 2009)

dorsocentral bristle, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

egg chamber | germline clone, with Scer\GAL4^- :

(Jankovics et al., 2001)

embryonic dorsal epidermis

(Sasikumar and Roy, 2009)

embryonic dorsal epidermis (with Df(3R)e-R1)

(Sasikumar and Roy, 2009)

embryonic leading edge cell

(Sasikumar and Roy, 2009)

embryonic leading edge cell (with Df(3R)e-R1)

(Sasikumar and Roy, 2009)

eye photoreceptor cell | somatic clone

(Alone et al., 2005)

interommatidial bristle (with Rab11^mo)

(Alone et al., 2005)

interommatidial bristle | somatic clone

(Alone et al., 2005)

macrochaeta (with Rab11^93Bi), with Scer\GAL4^- :

(Jankovics et al., 2001)

multivesicular body & eye photoreceptor cell | somatic clone, with Scer\GAL4^- :

(Satoh et al., 2005)

Nebenkern (with Rab11^93Bi)

(Giansanti et al., 2007)

ommatidium (with Rab11^mo)

(Alone et al., 2005)

rhabdomere | somatic clone, with Scer\GAL4^- :

(Satoh et al., 2005)

scutellar bristle, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

spermatid (with Rab11^93Bi)

(Giansanti et al., 2007)

tormogen cell, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

Detailed Description

Statement

Reference

Approximately 50% of homozygous stage 13-14 embryos have abnormally shaped and less elongated lateral epidermal cells than normal. 35% of homozygous embryos show intermittent loss of PTyr-enriched actin-nucleating centers that form between neighbouring dorsal-most epidermal cells. Some of the mutant embryos show widening of some of the dorsal-most epidermal cells and disruptions in the leading edge. The embryos show partial to near complete disruptions of the cell boundaries of the amnioserosa. Rab11^EP3017/Df(3R)e-R1 embryos show similar defects in the dorsal-most epidermal cells, amnioserosa and the lateral epidermis, at a comparable penetrance.

(Sasikumar and Roy, 2009)

Testes from Rab11^93Bi/Rab11^EP3017 larvae have spermatids composed of an abnormally large Nebenkern associated with multiple nuclei.

(Giansanti et al., 2007)

Homozygous clones in the eye lack all photoreceptor cells and lack interommatidial bristles.

Adult Rab11^mo/Rab11^EP3017 escapers have eye defects including fused ommatidia and missing bristles.

(Alone et al., 2005)

Few mutant photoreceptors are seen in mosaic eyes, probably reflecting a cell-essential role for Rab11. These mutant photoreceptors are reduced in size and a profusion of vesicles fills the photoreceptor cytoplasm. The normal multivesicular bodies (MVBs) are missing in these mutant cells, but infrequently, irregular vesicular organelles resembling defective MVBs are seen. The ER and Golgi have a normal appearance in these cells.

(Satoh et al., 2005)

Egg chambers older than stage 4 are never detected in females containing homozygous germ-line clones.

(Jankovics et al., 2001)

Expression of Rab11^EP3017 under the control of Scer\GAL4^sca-537.4 results in the loss of scutellar and dorsocentral macrochaetae. Sensory organs with no socket, one shaft and one sheath cell are seen.

(Abdelilah-Seyfried et al., 2000)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Rab11^EP3017, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by N[+]/N^55e11

(Abdelilah-Seyfried et al., 2000)

NOT suppressed by

Statement

Reference

Rab11^EP3017, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by N[+]/N^55e11

(Abdelilah-Seyfried et al., 2000)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Rab11^EP3017/Scer\GAL4^sca-537.4 is a non-enhancer of phenotype of N^55e11

(Abdelilah-Seyfried et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

(Jankovics et al., 2001)

(Jankovics et al., 2001)

(Jankovics et al., 2001)

(Jankovics et al., 2001)

Rescued by

Rab11^EP3017 is rescued by Rab11^+t6.3.A

(Alone et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

42708

w^*; P{EP}Rab11^EP3017/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Comments

Order of alleles (based on transheterozygous sterile phenotype): Rab11^93Bi < Rab11^j2D1 < Rab11^EP3017 = Rab11^ETo11 = Rab11^ETo3 < Df(3R)e-N19.

(Jankovics et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

EP(3)3017

(Abdelilah-Seyfried et al., 2000)

Rab11^EP(3)3017

(Alone et al., 2005)

Rab11^EP3017

(Sasikumar and Roy, 2009)

rab11^EP3017

(Krämer et al., 2019, Giansanti et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections