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General Information
Symbol
Dmel\bchsEP2299
Species
D. melanogaster
Name
FlyBase ID
FBal0119642
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
EP(2)2299, EP(2L)2299, bchs4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Single insertion within the first intron, upstream of the bchs open reading frame.

The P{EP} element is inserted 829 nucleotides 5' to the predicted ATG start codon of CG14001.

Insertion components
P{EP}EP2299
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Overexpression of bchsEP2299 under the control of Scer\GAL4GMR.PF engenders a reduced eye phenotype.

Overexpression of bchsEP2299 in the eye under the control of Scer\GAL4GMR.PF results in a rough eye phenotype and age related neuronal degeneration.

Expression of bchsEP2299 in the developing nervous system, driven by Scer\GAL4elav-C155 results in a 14.6% increase in brain volume when compared to controls.

Flies expressing bchsEP2299 under the control of Scer\GAL4GMR.PF have a roughened eye surface and a slight decrease in overall eye size compared to controls.

Animals expressing bchsEP2299 under the control of Scer\GAL4GMR.PF show alterations in the neural projection patterns of photoreceptor cells compared to wild-type in mid-pupae; the number and basic array of R7 and R8 axonal projections remain relatively normal, but there is a loss of growth cone morphology, premature termination of developing synapses and axonal swellings or varicosities are seen.

Expression of bchsEP2299 in third instar eye discs, under the control of Scer\GAL4GMR.PF, does not affect the patterning of the disc. Expression in the developing retina results in photoreceptor growth cones with an unusually bulbous central region that are less expanded than wild-type growth cones. However, Scer\GAL4GMR.PF>bchsEP2299 growth cones appear to grow normally towards their targets. At the adult stage, flies of this genotype have small, glazed eyes that lack distinct ommatidia compared to those of wild-type flies.

Expression of bchsEP2299 under the control of Scer\GAL4GMR.PF results in smaller eyes than normal which have a glossy appearance.

Expression of bchsEP2299 under the control of Scer\GAL4GMR.PU results in a rough eye phenotype.

bchsEP2299 homozygotes and bchsEP2299/Df(2L)cl7 flies have a reduced average lifespan compared to wild-type. These flies also have age-dependent ubiquitinated aggregates, that appear to be insoluble, throughout the CNS.

Mutant larvae expressing bchsEP2299 under the control of Scer\GAL4elav-C155 have reduced or abnormal synapses.

Mutant larvae expressing bchsEP2299 under the control of Scer\GAL4elav-C155 have altered ISN synapses with abnormal bulges at the junctions between the axons and the synaptic branches.

Expression of bchsEP2299 under the control of Scer\GAL4sca-537.4 results in severe loss of microchaetae on the abdomen and loss of scutellar and dorsocentral macrochaetae. Sensory organs with one socket, no shaft and one sheath cell are seen.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, bchsEP2299 has visible phenotype, enhanceable by alphaTub84B[+]/αTub84B5

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, bchsEP2299 has visible phenotype, enhanceable by alphaTub84B[+]/αTub84B7

NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Phenotype Manifest In
Enhanced by
Statement
Reference

Scer\GAL4GMR.PU, bchsEP2299 has eye phenotype, enhanceable by GgaΔ

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, bchsEP2299 has eye phenotype, enhanceable by alphaTub84B[+]/αTub84B5

Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, bchsEP2299 has eye phenotype, enhanceable by alphaTub84B[+]/αTub84B7

Suppressed by
Enhancer of
NOT Enhancer of
Statement
Reference
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Homo- and hemizygous LerpF6 enhances the reduced eye phenotype caused by bchsEP2299 overexpression.

The bchsEP2299-overexpression phenotype in the eye is enhanced when GgadsRNA.Scer\UAS.1 is co-expressed under the control of Scer\GAL4GMR.PF resulting in a reduction in eye size and a marked bleaching in the middle of the eye.

rb1 is a complex modifier of the eye phenotype caused by expression of bchsEP2299 under the control of Scer\GAL4GMR.PF.

g1 is a complex modifier of the eye phenotype caused by expression of bchsEP2299 under the control of Scer\GAL4GMR.PF.

cm1 is a complex modifier of the eye phenotype caused by expression of bchsEP2299 under the control of Scer\GAL4GMR.PF.

Flies that are mutant for Df(3R)e-R1 show a strong enhancement of the Scer\GAL4GMR.PF>bchsEP2299 overexpression phenotype.

Rab11 heterozygotes act as dominant enhancers of the Scer\GAL4GMR.PF>bchsEP2299 eye phenotype, resulting in adults with severely reduced eyes. The following alleles cause this enhancement: Rab11j2D1, Rab1193Bi, Rab11ex1, Rab11ex2, Rab11ETo11, and Rab11ETo3.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (17)