Allele Dmel\CycD^Scer\UAS.cDa

General Information
Symbol	Dmel\CycD^Scer\UAS.cDa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Datar	FlyBase ID	FBal0119681
Feature type	allele	Associated gene	Dmel\CycD

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Datar et al., 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of a 2.1 kb Cdk4 cDNA derived from LD05713. (Datar et al., 2000)
Carried in construct	P{UAS-CycD.D} (Frei and Edgar, 2004, Berry and Baehrecke, 2007, Gilboa and Lehmann, 2006, Chen et al., 2003, Duman-Scheel et al., 2002, Datar et al., 2000, de la Cova et al., 2004, Frei et al., 2005, Shcherbata et al., 2004, Bateman and McNeill, 2004, Tapon et al., 2001, Nolo et al., 2006, Secombe et al., 2007, Baonza and Freeman, 2005, Datar et al., 2006, Ji et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	wild-type, with Scer\GAL4^Act5C.PP (Datar et al., 2000)
Phenotype Manifest In

Detailed Description
	Statement Reference Cells expressing Cdk4^Scer\UAS.cMa and analysed using FACS technology show no detectable phenotype. When CycD^Scer\UAS.cDa expression is driven by Scer\GAL4^GMR.PF or Scer\GAL4^hs.2sev, no eye overgrowth is seen. (Datar et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has visible phenotype, non-enhanceable by lid¹⁰⁴²⁴ (Secombe et al., 2007) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has visible phenotype, non-enhanceable by lid^k06801 (Secombe et al., 2007) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has visible phenotype, non-enhanceable by lid^{Scer\UAS.P\T.cSa}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Secombe et al., 2007)
Suppressed by
Statement Reference Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^Act5C.PP has increased cell number \| somatic clone phenotype, suppressible by mRpL12¹⁰⁵³⁴ (Frei et al., 2005) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell size phenotype, suppressible by Df(3L)Scf-R6 (Frei et al., 2005) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell size phenotype, suppressible by mRpL12¹⁰⁵³⁴ (Frei et al., 2005) CycD^Scer\UAS.cDa, Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has visible phenotype, suppressible by Cdk4^Scer\UAS.cMa, Scer\GAL4^ey.PH (Tapon et al., 2001)
NOT suppressed by
Statement Reference Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by lid¹⁰⁴²⁴ (Secombe et al., 2007) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by lid^k06801 (Secombe et al., 2007) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by lid^{Scer\UAS.P\T.cSa}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Secombe et al., 2007)
Suppressor of
Statement Reference CycD^Scer\UAS.cDa, Scer\GAL4^ey.PH is a suppressor of visible phenotype of Rbf^Scer\UAS.cDa, Scer\GAL4^ey.PH (Datar et al., 2006) Scer\GAL4^GMR.PU, CycD^Scer\UAS.cDa/Cdk4^Scer\UAS.cMa is a suppressor of visible phenotype of E2f^{dsRNA.Scer\UAS.cJa}, Scer\GAL4^GMR.PU (Ji et al., 2012)
Other
Statement Reference Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^Act5C.PP has cell growth defective \| somatic clone phenotype (de la Cova et al., 2004, de la Cova et al., 2004) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^Act5C.PP has increased cell number \| somatic clone phenotype (Frei et al., 2005, Frei et al., 2005, Frei and Edgar, 2004, Frei and Edgar, 2004) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^Act5C.PP has increased cell size \| somatic clone phenotype (Bateman and McNeill, 2004, Bateman and McNeill, 2004) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^en-e16E has increased cell number phenotype (Frei and Edgar, 2004, Frei and Edgar, 2004) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell size phenotype (Frei et al., 2005, Datar et al., 2000, Frei et al., 2005, Datar et al., 2000, Datar et al., 2006) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has mitotic cell cycle defective phenotype (Datar et al., 2006) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has size defective phenotype (Datar et al., 2006) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has visible phenotype (Datar et al., 2006) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has increased cell number phenotype (Gilboa and Lehmann, 2006) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^unspecified has cell growth defective \| somatic clone phenotype (Frei et al., 2005, Frei et al., 2005) CycD^Scer\UAS.cDa, Scer\GAL4^Act5C.PP has endomitotic cell cycle defective \| somatic clone \| third instar larval stage phenotype (Datar et al., 2006, Datar et al., 2006) CycD^Scer\UAS.cDa, Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has visible phenotype (Tapon et al., 2001)
Phenotype Manifest In
NOT Enhanced by
Statement Reference Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by lid¹⁰⁴²⁴ (Secombe et al., 2007) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by lid^k06801 (Secombe et al., 2007) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by lid^{Scer\UAS.P\T.cSa}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Secombe et al., 2007)
Suppressed by
Statement Reference Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, suppressible by Df(3L)Scf-R6 (Frei et al., 2005) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, suppressible by Df(3L)Scf-R11 (Frei et al., 2005) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, suppressible by mRpL12¹⁰⁵³⁴ (Frei et al., 2005) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible \| partially by Hph[+]/Hph⁰²²⁵⁵ (Frei and Edgar, 2004) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible \| partially by Hph[+]/Hph^S030304 (Frei and Edgar, 2004) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Df(3L)Scf-R6 (Frei et al., 2005) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Df(3L)Scf-R11 (Frei et al., 2005) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by mRpL12¹⁰⁵³⁴ (Frei et al., 2005) CycD^Scer\UAS.cDa, Scer\GAL4^ey.PH, Tsc1^Scer\UAS.cTa, gig^Scer\UAS.cTa/gig^Scer\UAS.cTa has eye phenotype, suppressible by Cdk4^Scer\UAS.cMa, Scer\GAL4^ey.PH (Tapon et al., 2001)
NOT suppressed by
Statement Reference Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Df(3L)29A6 (Frei et al., 2005) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by lid¹⁰⁴²⁴ (Secombe et al., 2007) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by lid^k06801 (Secombe et al., 2007) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by lid^{Scer\UAS.P\T.cSa}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Secombe et al., 2007) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by Df(3L)29A6 (Frei et al., 2005)
Enhancer of
Statement Reference CycD^Scer\UAS.cDa/Cdk4^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye disc phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, os^Scer\UAS.cCa (Chen et al., 2003) CycD^Scer\UAS.cDa/Cdk4^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, os^Scer\UAS.cCa (Chen et al., 2003) CycD^Scer\UAS.cDa/Cdk4^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, os^Scer\UAS.cCa (Chen et al., 2003)
Suppressor of
Statement Reference CycD^Scer\UAS.cDa, Scer\GAL4^ey.PH is a suppressor of eye phenotype of Rbf^Scer\UAS.cDa, Scer\GAL4^ey.PH (Datar et al., 2000, Datar et al., 2006) CycD^Scer\UAS.cDa/Cdk4^Scer\UAS.cMa, Scer\GAL4^Act5C.PP is a suppressor of wing disc \| somatic clone phenotype of Scer\GAL4^Act5C.PP, ptc^Scer\UAS.cCa (Duman-Scheel et al., 2002) Scer\GAL4^ey.PH, CycD^Scer\UAS.cDa/Cdk4^{D175N.Scer\UAS.T:Hsap\MYC} is a suppressor \| partially of eye phenotype of Rbf^Scer\UAS.cDa, Scer\GAL4^ey.PH (Datar et al., 2000) Scer\GAL4^ey.PH, CycD^Scer\UAS.cDa/Cdk4^Scer\UAS.cMa is a suppressor of eye phenotype of Rbf^Scer\UAS.cDa, Scer\GAL4^ey.PH (Datar et al., 2000) Scer\GAL4^GMR.PU, CycD^Scer\UAS.cDa/Cdk4^Scer\UAS.cMa is a suppressor of eye phenotype of E2f^{dsRNA.Scer\UAS.cJa}, Scer\GAL4^GMR.PU (Ji et al., 2012)
Other
Statement Reference Cdk4^{D175N.Scer\UAS.T:Hsap\MYC}, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype (Datar et al., 2000) Cdk4^{D175N.Scer\UAS.T:Hsap\MYC}, CycD^Scer\UAS.cDa, Scer\GAL4^hs.2sev has eye phenotype (Datar et al., 2000) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^Act5C.PP has cone cell phenotype (Datar et al., 2000) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^Act5C.PP has embryonic/larval fat body \| somatic clone \| third instar larval stage phenotype (Datar et al., 2006) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^Act5C.PP has eye photoreceptor cell phenotype (Datar et al., 2000) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^Act5C.PP has interommatidial bristle phenotype (Datar et al., 2000) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^Act5C.PP has ommatidium phenotype (Datar et al., 2000) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^Act5C.PP has primary pigment cell phenotype (Datar et al., 2000) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^Act5C.PP has wing \| somatic clone phenotype (de la Cova et al., 2004, de la Cova et al., 2004) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^Act5C.PP has wing disc \| somatic clone \| third instar larval stage phenotype (Datar et al., 2006) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^dpp.blk1 has wing \| somatic clone phenotype (de la Cova et al., 2004, de la Cova et al., 2004) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^en-e16E has wing phenotype (Frei and Edgar, 2004) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype (Frei et al., 2005, Frei et al., 2005, Datar et al., 2000, Datar et al., 2006) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye photoreceptor cell phenotype (Datar et al., 2000) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has interommatidial bristle phenotype (Frei and Edgar, 2004, Frei and Edgar, 2004) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has mitotic cell cycle & eye disc \| posterior phenotype (Datar et al., 2000) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype (Frei et al., 2005, Frei et al., 2005, Frei and Edgar, 2004, Datar et al., 2000, Frei and Edgar, 2004, Datar et al., 2006) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has rhabdomere phenotype (Datar et al., 2000) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^hs.2sev has eye phenotype (Datar et al., 2000) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^hs.2sev has ommatidium phenotype (Datar et al., 2000) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has germline stem cell phenotype (Gilboa and Lehmann, 2006) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^unspecified has mitochondrion phenotype (Frei et al., 2005, Frei et al., 2005) Cdk4^Scer\UAS.cMa, CycD^Scer\UAS.cDa, Scer\GAL4^αTub84B.PC has wing \| somatic clone phenotype (de la Cova et al., 2004, de la Cova et al., 2004) Cdk4^{Scer\UAS.T:Hsap\MYC}, CycD^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye disc & photoreceptor cell phenotype (Chen et al., 2003, Chen et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference The strong rough eye phenotype seen in flies expressing multiple copies of E2f[dsRNA.Scer\UAS.cJa] under the control of Scer\GAL4[GMR.PU] is suppressed if they are also simultaneously co-expressing both Cdk4[Scer\UAS.cMa] and CycD[Scer\UAS.cDa]. (Ji et al., 2012) Co-expression of CycD^Scer\UAS.cDa and Cdk4^Scer\UAS.cMa under the control of Scer\GAL4^fkh.PH does not prevent the salivary gland degradation that is seen during the pupal stage in normal development. (Berry and Baehrecke, 2007) Co-expression of Cdk4[Scer\UAS.cMa] and CycD[Scer\UAS.cDa] under the control of Scer\GAL4[GMR.PF] results in enlargement of the eye together with an increase in ommatidial size. Cells from third larval instar eye discs co-expressing Cdk4[Scer\UAS.cMa] and CycD[Scer\UAS.cDa] under the control of Scer\GAL4[GMR.PF] are larger than wild-type controls, and there is an increase in cells in S and G[[2]/M compared to wild type. Co-expression of CycD[Scer\UAS.cDa] suppresses the small eye phenotype caused by expression of Rbf[Scer\UAS.cDa] under the control of Scer\GAL4[ey.PH]. Clones of cells in the third larval instar fat body co-expressing CycD[Scer\UAS.cDa] and Cdk4[Scer\UAS.cMa] under the control of Scer\GAL4[Act5C.PP] are larger than wild-type controls and contain more DNA. Clones of cells in the wing disc co-expressing CycD[Scer\UAS.cDa] and Cdk4[Scer\UAS.cMa] under the control of Scer\GAL4[Act5C.PP] show a 60-70% increase in median clone size compared to wild-type controls. (Datar et al., 2006) Expression of CycD^Scer\UAS.cDa and Cdk4^Scer\UAS.cMa, both under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in extensive overproliferation of primordial germ cells, without any qualitative loss of intermingled somatic cells. (Gilboa and Lehmann, 2006) When CycD^Scer\UAS.cDa and Cdk4^Scer\UAS.cMa are driven by Scer\GAL4^GMR.PF mutant eyes have bigger ommatidia and a rough appearance. This phenotype is suppressed by Df(3L)Scf-R6 or Df(3L)Scf-R11, but not Df(3L)29A6. When CycD^Scer\UAS.cDa and Cdk4^Scer\UAS.cMa are driven by Scer\GAL4^GMR.PF, eye imaginal disc cells exhibit an average increase in cell size. This effect is suppressed by Df(3L)Scf-R6 or mRpL12¹⁰⁵³⁴. The eye phenotypes seen in CycD^Scer\UAS.cDa, Cdk4^Scer\UAS.cMa (driven by Scer\GAL4^GMR.PF) animals is suppressed by mRpL12¹⁰⁵³⁴. This suppression effect is counteracted by the addition of mRpL12^{Scer\UAS.P\T.T:Avic\GFP-EGFP}. When CycD^Scer\UAS.cDa and Cdk4^Scer\UAS.cMa are induced in random clones (driven by Scer\GAL4^Act5C.PP), a 60% increase in clone areas is seen. Since these cells also proliferate at an increased rate, they retain their normal size. This effect is also suppressed by mRpL12¹⁰⁵³⁴. When CycD^Scer\UAS.cDa and Cdk4^Scer\UAS.cMa are expressed in fat body cells additional rounds of endoreduplication are stimulated in normally fed animals and also to a greater extent in starved animals. This effect is also suppressed by mRpL12¹⁰⁵³⁴. When CycD^Scer\UAS.cDa and Cdk4^Scer\UAS.cMa are expressed in fat body cell or tracheal cells an apparent increase in mitochondrial activity is seen. The overgrowth defects associated with over-expression of CycD^Scer\UAS.cDa and Cdk4^Scer\UAS.cMa are suppressed by hypoxia and mitochondrial inhibitors. (Frei et al., 2005) Clones in the eye disc co-expressing CycD^Scer\UAS.cDa and Cdk4^Scer\UAS.cMa under the control of Scer\GAL4^Act5C.PP result in larger cells than normal. (Bateman and McNeill, 2004) Somatic clones expressing CycD^Scer\UAS.cDa and Cdk4^Scer\UAS.cMa (driven by Scer\GAL4^αTub84B.PC, in the wing disc) are significantly larger than controls. When somatic clones expressing CycD^Scer\UAS.cDa and Cdk4^Scer\UAS.cMa driven by Scer\GAL4^Act5C.PP or Scer\GAL4^dpp.blk1 leads to wings that are significantly larger than wild-type. (de la Cova et al., 2004) wing Cdk4^Scer\UAS.cMa; CycD^Scer\UAS.cDa; Scer\GAL4^GMR.PF flies have enlarged ommatidia and lengthened interommatidial bristles. These phenotypes are partially suppressed by Df(3R)6-7/+, Df(3R)3-4/+, Hph^S030304/+ or Hph⁰²²⁵⁵/+ but not Df(3R)110/+. Somatic clones of Cdk4^Scer\UAS.cMa; CycD^Scer\UAS.cDa; Scer\GAL4^Act5C.PP cells in the wing disc are 75% larger than control clones 48 hours after induction at 66 hours after egg laying. This phenotype is suppressed by Hph⁰²²⁵⁵/+. The cells in these enlarged clones are not significantly larger than wild-type and do not differ significantly from wild-type controls in their cell cycle phasing. Cell size and cell cycle phasing in these clones is also unaffected bu the presence of Hph⁰²²⁵⁵/+. CycD^Scer\UAS.cDa; Cdk4^Scer\UAS.cMa; Scer\GAL4^Act5C.PP somatic clones do not grow any larger when Hph^EP3200 is present unless BacA\p35^Scer\UAS.cHa is also present, in which case the effects on growth are additive. The wings of CycD^Scer\UAS.cDa; Cdk4^Scer\UAS.cMa; Scer\GAL4^en-e16E Hph^EP3200; Scer\GAL4^en-e16E adults have an enlarged posterior compartment but no change in trichome density, indicating increased cell number without cell size change. (Frei and Edgar, 2004) Co-expression of CycD^Scer\UAS.cDa with Cdk4^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^GMR.PF blocks photoreceptor differentiation in the third instar eye disc. The eye and eye disc overgrowth phenotypes seen in os^Scer\UAS.cCa; Scer\GAL4^GMR.PF flies, are dramatically enhanced by CycD^Scer\UAS.cDa with Cdk4^{Scer\UAS.T:Hsap\MYC}: All ommatidia are dramatically enlarged in these eyes, and the entire eye bulges out of the head. The increase in ommatidial numbers seen in os^Scer\UAS.cCa; Scer\GAL4^GMR.PF eye discs is also enhanced. (Chen et al., 2003) Co-expression of Cdk4^Scer\UAS.cMa and CycD^Scer\UAS.cDa suppresses the inhibition of growth seen in clones in the wing discs expressing ptc^Scer\UAS.cCa under the control of Scer\GAL4^Act5C.PP. (Duman-Scheel et al., 2002) Expression of both Tsc1^Scer\UAS.cTa and gig^Scer\UAS.cTa in the eye under the control of Scer\GAL4^ey.PH results in a much smaller eye than normal. Coexpression of both CycD^Scer\UAS.cDa and Cdk4^Scer\UAS.cMa fully suppresses the phenotype. Coexpression of CycD^Scer\UAS.cDa partially suppresses the phenotype. (Tapon et al., 2001) When clones expressing Cdk4^Scer\UAS.cMa and CycD^Scer\UAS.cDa (driven by Scer\GAL4^Act5C.PP) are examined, although there is no alteration in cell cycle phasing or cell size, there is an increase in the number of cells per clone, when compared to wild-type sister clones - indicating an accelerated cell division. Adult wings containing these clones show no gross abnormalities in overall shape, venation, bristle or trichome patterning. There are also no significant changes in cell morphology or cell density. In adult eyes that clonally overexpress Cdk4^Scer\UAS.cMa and CycD^Scer\UAS.cDa (under the control of Scer\GAL4^Act5C.PP), there is a striking enlargement of ommatidia. This effect is cell autonomous. There is also a mild disorganisation in the regular arrangement of ommatidia, but no specific defects in cell differentiation or overall patterning. Hypertrophy occurs in several cell types and structures, including primary pigment and cone cells, photoreceptors and interommatidial bristles. In clones in ommatidia, 5 cone cells are seen instead of the normal 4. When Cdk4^Scer\UAS.cMa and CycD^Scer\UAS.cDa are driven by either Scer\GAL4^hs.2sev or Scer\GAL4^GMR.PF, all ommatidia are enlarged, as is the entire eye, which bulges out in an ominous fashion. Examination of Cdk4^Scer\UAS.cMa,CycD^Scer\UAS.cDa,Scer\GAL4^GMR.PF flies reveals enlarged photoreceptor cell bodies and rhabdomeres and excessive accumulations of actin. The eye size phenotype is dose dependant in relation to CycD^Scer\UAS.cDa. When Cdk4^Scer\UAS.cMa and CycD^Scer\UAS.cDa are driven by Scer\GAL4^GMR.PF, and the cells of the eye disc analysed using FACS analysis, a significantly increased fraction of S and G2 phase cells was seen in cells from posterior to the morphogenetic furrow. Also a significant increase in cell size is seen in these posterior eye cells. Co-expression of Rbf^Scer\UAS.cDa, Cdk4^Scer\UAS.cMa and CycD^Scer\UAS.cDa in clones (driven by Scer\GAL4^Act5C.PP) produces cells that cycle more rapidly than cells expressing Rbf^Scer\UAS.cDa alone. Clones have fewer cells than wild-type controls, but they encompass substantially more area. Co-expression of Rbf^Scer\UAS.cDa and CycD^Scer\UAS.cDa when driven byor Scer\GAL4^GMR.PF no eye phenotype is seen. (Datar et al., 2000)
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Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	CycD^Scer\UAS.cDa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Datar (Datar et al., 2000)
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References ( 16 )
Research paper	Ji et al., 2012, G3 (Bethesda) 2(12): 1651--1660 In Vivo Regulation of E2F1 by Polycomb Group Genes in Drosophila. [FBrf0220353] Berry and Baehrecke, 2007, Cell 131(6): 1137--1148 Growth arrest and autophagy are required for salivary gland cell degradation in Drosophila. [FBrf0200408] Secombe et al., 2007, Genes Dev. 21(5): 537--551 The Trithorax group protein Lid is a trimethyl histone H3K4 demethylase required for dMyc-induced cell growth. [FBrf0193047] Datar et al., 2006, Cell Cycle 5(6): 647--652 Mammalian cyclin D1/Cdk4 complexes induce cell growth in Drosophila. [FBrf0189936] Gilboa and Lehmann, 2006, Nature 443(7107): 97--100 Soma-germline interactions coordinate homeostasis and growth in the Drosophila gonad. [FBrf0193494] Nolo et al., 2006, Curr. Biol. 16(19): 1895--1904 The bantam microRNA is a target of the hippo tumor-suppressor pathway. [FBrf0194704] Baonza and Freeman, 2005, Dev. Cell 8(4): 529--539 Control of cell proliferation in the Drosophila eye by notch signaling. [FBrf0187403] Frei et al., 2005, EMBO J. 24(3): 623--634 The Drosophila mitochondrial ribosomal protein mRpL12 is required for Cyclin D/Cdk4-driven growth. [FBrf0183949] Bateman and McNeill, 2004, Cell 119(1): 87--96 Temporal control of differentiation by the insulin receptor/tor pathway in Drosophila. [FBrf0180028] de la Cova et al., 2004, Cell 117(1): 107--116 Drosophila myc regulates organ size by inducing cell competition. [FBrf0174444] Frei and Edgar, 2004, Dev. Cell 6(2): 241--251 Drosophila cyclin D/Cdk4 requires Hif-1 prolyl hydroxylase to drive cell growth. [FBrf0174538] Shcherbata et al., 2004, Development 131(13): 3169--3181 The mitotic-to-endocycle switch in Drosophila follicle cells is executed by Notch-dependent regulation of G1/S, G2/M and M/G1 cell-cycle transitions. [FBrf0179442] Chen et al., 2003, Dev. Cell 4(2): 179--190 Cyclin d-cdk4 and cyclin e-cdk2 regulate the Jak/STAT signal transduction pathway in Drosophila. [FBrf0155684] Duman-Scheel et al., 2002, Nature 417(6886): 299--304 Hedgehog regulates cell growth and proliferation by inducing Cyclin D and Cyclin E. [FBrf0149123] Tapon et al., 2001, Cell 105(3): 345--355 The Drosophila tuberous sclerosis complex gene homologs restrict cell growth and cell proliferation. [FBrf0135684] Datar et al., 2000, EMBO J. 19(17): 4543--4554 The Drosophila cyclin D-cdk4 complex promotes cellular growth. [FBrf0129782]

Allele Dmel\CycDScer\UAS.cDa

Allele Dmel\CycD^Scer\UAS.cDa