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General Information
Symbol
Dmel\αTub84BUASp.cGa.GFP(S65C)
Species
D. melanogaster
Name
FlyBase ID
FBal0119725
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
GFP-α-tubulin, GFP-tubulin, tubulin-GFP, UAS-tubulin-GFP, tub-GFP, UAS-GFP-α-tubulin, α-tubulin-GFP, P{UASp-GFPS65C-alphaTub84B}, UASp-GFP-α-tubulin, UAS-tub-GFP
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASp regulatory sequences drive expression of the N-terminal region of αTub84B (encompassing amino acid residue 2 up to the internal BamHI site) tagged with GFP(S65C).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Most embryos derived from females expressing αTub84BScer\UAS.P\T.T:Avic\GFP-S65C under the control of Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16 give rise to living larvae and also develop to adulthood. Defects in oogenesis are only occasionally seen.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of a single copy of αTub84BScer\UAS.P\T.T:Avic\GFP-S65C, in del2/del4 mutants, under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 exacerbates the ovarian phenotype, such that young flies contain only rudimentary ovaries with few developing cysts exhibiting disorganisation of interior microtubules in cells in region 1 of del2/del4 mutant germaria as compared to heterozygotes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
Comments
Comments

Driven by Scer\GAL4nos.UTR.T:Hsim\VP16 in the early embryo to use as a marker.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
GFP::α-tubulin
αTub84BScer\UAS.P\T.T:Avic\GFP-S65C
αTub84BScer\UAS.T:Avic\GFP-S65C
αTub84BUASp.cGa.GFP(S65C)
Name Synonyms
Secondary FlyBase IDs
    References (74)