FB2025_01 , released February 20, 2025
Allele: Dmel\αTub84BUASp.cGa.GFP(S65C)
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General Information
Symbol
Dmel\αTub84BUASp.cGa.GFP(S65C)
Species
D. melanogaster
Name
FlyBase ID
FBal0119725
Feature type
allele
Associated gene
Dmel\αTub84B
Associated Insertion(s)
Carried in Construct
P{UASp-GFPS65C-αTub84B}
Also Known As
GFP-α-tubulin, GFP-tubulin, tubulin-GFP, UAS-tubulin-GFP, UAS-GFP-αTub84B, UAS-α-tubulin-GFP, UAS-GFP-α-tubulin84B, UAS-GFP-α-tub84B, tub-GFP, UAS-GFP-α-tubulin
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Grieder et al., 2000)
Progenitor genotype
Carried in construct
P{UASp-GFPS65C-αTub84B}
Cytology
Description

UASp regulatory sequences drive expression of the N-terminal region of αTub84B (encompassing amino acid residue 2 up to the internal BamHI site) tagged with GFP(S65C).

(Grieder et al., 2000)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
αTub84B
Tagged with
GFP(S65C)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Most embryos derived from females expressing αTub84BScer\UAS.P\T.T:Avic\GFP-S65C under the control of Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16 give rise to living larvae and also develop to adulthood. Defects in oogenesis are only occasionally seen.

      (Grieder et al., 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of a single copy of αTub84BScer\UAS.P\T.T:Avic\GFP-S65C, in del2/del4 mutants, under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 exacerbates the ovarian phenotype, such that young flies contain only rudimentary ovaries with few developing cysts exhibiting disorganisation of interior microtubules in cells in region 1 of del2/del4 mutant germaria as compared to heterozygotes.

      (Wehr et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (3)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Driven by Scer\GAL4nos.UTR.T:Hsim\VP16 in the early embryo to use as a marker.

      (Trieselmann and Wilde, 2002)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      GFP::α-tubulin
      (Serbus et al., 2005)
      αTub84BScer\UAS.P\T.T:Avic\GFP-S65C
      αTub84BScer\UAS.T:Avic\GFP-S65C
      αTub84BUASp.cGa.GFP(S65C)
      Name Synonyms
      Secondary FlyBase IDs
        References (93)