tergal depressor of trochanter muscle motor neuron & dendrite, with Scer\GAL4shakB.lethal.4.1
Misexpression of roboScer\UAS.T:Hsap\MYC in the contralateral eagle (eg) neurons under the control of Scer\GAL4eg-Mz360 prevents the posterior subset of eg axons, but not the anterior, from crossing the midline.
Single cell leg motoneuron clones expressing roboScer\UAS.T:Hsap\MYC induced 48 hr after hatching have the following phenotypes: a general shift of the arborization away from the midline with an increase in the posterior lateral regions of the neuropil; the mean centre of mass is shifted away from the midline compared to controls; the mean 33rd percentile of arborization is shifted away from the midline.
Single cell leg motoneuron clones expressing roboScer\UAS.T:Hsap\MYC induced 96 hr after hatching have the following phenotypes: a general shift of the arborization away from the midline; the mean centre of mass is shifted away from the midline compared to controls; the mean 33rd percentile of arborization is shifted away from the midline.
Expression of roboScer\UAS.T:Hsap\MYC under the control of Scer\GAL415J2 results in misrouting of the dMP2 neuron before the normal posterior turning in 8.16% of cases.
Expression of roboScer\UAS.T:Hsap\MYC under the control of Scer\GAL4A307 results in anatomical and physiological defects in the giant fiber (GF) system; 100% of GFs are deflected laterally, a proportion show a "bendless"-like phenotype (the end of the GF is either swollen or tapered), and the latency (a measure of synaptic function) of these axons is increased compared to wild type. Expression of roboScer\UAS.T:Hsap\MYC under the control of Scer\GAL4c17 results in anatomical and physiological defects in the giant fiber (GF) system; a proportion of GFs are deflected laterally, a proportion show a "bendless"-like phenotype (the end of the GF is either swollen or tapered), and the latency (a measure of synaptic function) of these axons is slightly increased compared to wild type. Ectopic expression of roboScer\UAS.T:Hsap\MYC, under the control of Scer\GAL4shakB.lethal.4.1, in the tergotrochanteral motor neurons results in disrupted tergal depressor of trochanter muscle motor neuron & dendrites with a penetrance of 100%. The latency (a measure of synaptic function) of these axons is increased compared to wild type.
robo1UAS.Tag:MYC, Scer\GAL415J2 is a suppressor of abnormal neuroanatomy | embryonic stage phenotype of GαqQ203L.UAS, Scer\GAL415J2
robo1UAS.Tag:MYC, Scer\GAL415J2 is a suppressor of dMP2 neuron phenotype of GαqQ203L.UAS, Scer\GAL415J2
Co-expression of roboScer\UAS.T:Hsap\MYC suppresses the defects in the dMP2 neuron which are caused by expression of Gα49BQ203L.Scer\UAS under the control of Scer\GAL415J2.
robo1UAS.Tag:MYC/Scer\GAL4elav.PLu partially rescues robo1unspecified
robo1UAS.Tag:MYC/Scer\GAL415J2 partially rescues robo11
Pan-neuronal expression of roboScer\UAS.T:Hsap\MYC under the control of Scer\GAL4elav.PLu partially rescues the midline crossing phenotype seen in robounspecified stage 16 embryos. Some ectopic crossing of FasII axons is still seen.
Expression of roboScer\UAS.T:Hsap\MYC in the ap neurons under the control of Scer\GAL4ap-md544 rescues the midline crossing phenotype seen in robounspecified mutants.
Overexpression of roboScer\UAS.T:Hsap\MYC driven by Scer\GAL4tim.PE in a roboBG01092 homozygous background rescues the short-period phenotype when four copies of roboScer\UAS.T:Hsap\MYC but not when only two are expressed.
Expression of roboScer\UAS.T:Hsap\MYC under the control of Scer\GAL415J2 rescues the contralateral misprojection phenotype of dMP2 axons seen in robo1 embryos, while many surrounding neurons misproject contralaterally.
