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General Information
Symbol
Dmel\robo1UAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0120361
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-robo-myc
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of robo1 tagged with Tag:MYC.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Misexpression of roboScer\UAS.T:Hsap\MYC in the contralateral eagle (eg) neurons under the control of Scer\GAL4eg-Mz360 prevents the posterior subset of eg axons, but not the anterior, from crossing the midline.

Single cell leg motoneuron clones expressing roboScer\UAS.T:Hsap\MYC induced 48 hr after hatching have the following phenotypes: a general shift of the arborization away from the midline with an increase in the posterior lateral regions of the neuropil; the mean centre of mass is shifted away from the midline compared to controls; the mean 33rd percentile of arborization is shifted away from the midline.

Single cell leg motoneuron clones expressing roboScer\UAS.T:Hsap\MYC induced 96 hr after hatching have the following phenotypes: a general shift of the arborization away from the midline; the mean centre of mass is shifted away from the midline compared to controls; the mean 33rd percentile of arborization is shifted away from the midline.

Expression of roboScer\UAS.T:Hsap\MYC under the control of Scer\GAL415J2 results in misrouting of the dMP2 neuron before the normal posterior turning in 8.16% of cases.

Expression of roboScer\UAS.T:Hsap\MYC under the control of Scer\GAL4A307 results in anatomical and physiological defects in the giant fiber (GF) system; 100% of GFs are deflected laterally, a proportion show a "bendless"-like phenotype (the end of the GF is either swollen or tapered), and the latency (a measure of synaptic function) of these axons is increased compared to wild type. Expression of roboScer\UAS.T:Hsap\MYC under the control of Scer\GAL4c17 results in anatomical and physiological defects in the giant fiber (GF) system; a proportion of GFs are deflected laterally, a proportion show a "bendless"-like phenotype (the end of the GF is either swollen or tapered), and the latency (a measure of synaptic function) of these axons is slightly increased compared to wild type. Ectopic expression of roboScer\UAS.T:Hsap\MYC, under the control of Scer\GAL4shakB.lethal.4.1, in the tergotrochanteral motor neurons results in disrupted tergal depressor of trochanter muscle motor neuron & dendrites with a penetrance of 100%. The latency (a measure of synaptic function) of these axons is increased compared to wild type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of roboScer\UAS.T:Hsap\MYC suppresses the defects in the dMP2 neuron which are caused by expression of Gα49BQ203L.Scer\UAS under the control of Scer\GAL415J2.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Pan-neuronal expression of roboScer\UAS.T:Hsap\MYC under the control of Scer\GAL4elav.PLu partially rescues the midline crossing phenotype seen in robounspecified stage 16 embryos. Some ectopic crossing of FasII axons is still seen.

Expression of roboScer\UAS.T:Hsap\MYC in the ap neurons under the control of Scer\GAL4ap-md544 rescues the midline crossing phenotype seen in robounspecified mutants.

Overexpression of roboScer\UAS.T:Hsap\MYC driven by Scer\GAL4tim.PE in a roboBG01092 homozygous background rescues the short-period phenotype when four copies of roboScer\UAS.T:Hsap\MYC but not when only two are expressed.

Expression of roboScer\UAS.T:Hsap\MYC under the control of Scer\GAL415J2 rescues the contralateral misprojection phenotype of dMP2 axons seen in robo1 embryos, while many surrounding neurons misproject contralaterally.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
robo1Scer\UAS.T:Hsap\MYC
robo1UAS.Tag:MYC
roboScer\UAS.T:Hsap\MYC
Name Synonyms
Secondary FlyBase IDs
    References (8)