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General Information
Symbol
Dmel\dppUAS.T.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0120515
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Dpp-GFP, UAS-Dpp::GFP
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of dpp cDNA e5 that is tagged downstream of the predicted proteolytic cleavage site with EGFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The adulthood-only expression of dppScer\UAS.T.T:Avic\GFP-EGFP (using Gal80[ts] for the temporal regulation of expression) under the control of either Scer\GAL4esg.PU or Scer\GAL4Acp26Aa.PC leads to a significant increase in the size of secondary cell nuclei, but not of main cell nuclei, as compared to controls; the Scer\GAL4esg.PU-induced phenotype is cell autonomous.

Overexpression of dppScer\UAS.T.T:Avic\GFP-EGFP in AB cells using Scer\GAL4ptc-559.1 casues an increase in the size of the posterior compartment. Overexpression under the control of Scer\GAL4bi-omb-Gal4, however, results in a reduction in the differences between anterior and posterior compartments in the haltere discs.

Expression of dppScer\UAS.T.T:Avic\GFP-EGFP under the control of Scer\GAL4dpp.blk1 results in imaginal disc overgrowth.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement
Reference

dppUAS.T.EGFP/Scer\GAL4dpp.blk1 is a suppressor of dorsal mesothoracic disc & cytoskeleton phenotype of coroex6/coroex11

dppUAS.T.EGFP/Scer\GAL4dpp.blk1 is a suppressor of dorsal mesothoracic disc & cytoskeleton phenotype of coroex6

Other
Additional Comments
Genetic Interactions
Statement
Reference

Discs overexpressing dppScer\UAS.T.T:Avic\GFP-EGFP under the control of Scer\GAL4dpp.blk1 in coroex11 or coroex6 homozygous or heterozygous mutants or coroex6/coroex11 transheterozygous mutants are much larger than wild-type discs expressing dppScer\UAS.T.T:Avic\GFP-EGFP under the control of Scer\GAL4dpp.blk1. In coroex6 homozygous mutants and coroex6/coroex11 mutants, a partial rescue of the actin cytoskeleton and apicobasal polarity is seen in wing discs overexpressing dppScer\UAS.T.T:Avic\GFP-EGFP under the control of Scer\GAL4dpp.blk1.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

dppScer\UAS.T.T:Avic\GFP-EGFP rescues the wing phenotype of dppd8/dppd12 flies when expressed under the control of Scer\GAL4dpp.blk1; wing size and pattern are almost normal except that ectopic vein material is seen near vein 3 and the spacing between veins 3 and 4 is slightly increased.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
dppScer\UAS.T.T:Avic\GFP-EGFP
dppScer\UAS.T.T:Avic\GFP-F64L.S65T
dppUAS.T.EGFP
Name Synonyms
Secondary FlyBase IDs
    References (15)