Expression of a full length amn ORF is governed by UASt regulatory sequences.
memory defective, with Scer\GAL4c316, amnX8
memory defective, with Scer\GAL4Mz717, amnX8
amnX8;Scer\GAL4c316/amnScer\UAS.cWa and amnX8;Scer\GAL4Mz717/amnScer\UAS.cWa flies learn to avoid benzaldehyde significantly better than amnX8 flies, although their performance is still significantly worse than that of wild-type flies. In contrast, OCT immediate memory of amnX8 flies is indistinguishable from wild-type flies and amnX8;Scer\GAL4c316/amnScer\UAS.cWa and amnX8;Scer\GAL4Mz717/amnScer\UAS.cWa flies.
amnUAS.cWa/Scer\GAL4da.G32 rescues amnX8/amn28A
amnUAS.cWa/Scer\GAL4c316 rescues amn1
amnUAS.cWa/Scer\GAL4c316 rescues amnX8/amn1
amnUAS.cWa/Scer\GAL4c316 rescues amnX8
amnUAS.cWa/Scer\GAL4c316 rescues amnchpd
amnUAS.cWa/Scer\GAL4c309 fails to rescue amnX8
Scer\GAL4c747/amnUAS.cWa fails to rescue amnX8
The presence of amnScer\UAS.cWa under the control of Scer\GAL4da.G32 significantly rescues the delayed larval and adult response to noxious heat in amn28A/amnX8 females.