FlyBase Allele Report: Dmel\mTor[EP2353]

General Information

Symbol

Dmel\mTor^EP2353

Species

D. melanogaster

Name

FlyBase ID

FBal0120582

Feature type

allele

Associated gene

Dmel\mTor

Associated Insertion(s)

P{EP}mTor^EP2353

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-, Kraut et al., 2001, Oldham et al., 2000, Zhang et al., 2000)

Progenitor genotype

Associated Insertion(s)

P{EP}mTor^EP2353

Cytology

Description

The P{EP} insertion is located 262bp upstream of the putative translation start of Tor, and within the 5' UTR.

(Oldham et al., 2000)

The P{EP} insertion is located 74bp downstream of the transcription start site.

(Zhang et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:13,004,577..13,004,577 [-]

(Nowak et al., 2013, Zhang et al., 2006, Gene Disruption Project members, 2001-, Kraut et al., 2001, Oldham et al., 2000, Zhang et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

decreased body size

(Zhang et al., 2000)

Phenotype Manifest In

anterior fascicle & synapse, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

larva

(Zhang et al., 2000)

larval intersegmental nerve, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

Detailed Description

Statement

Reference

Mutant larvae expressing Tor^EP2353 under the control of Scer\GAL4^elav-C155 have reduced or abnormal synapses. Mutant larvae expressing Tor^EP2353 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects.

(Kraut et al., 2001)

Homozygotes hatch at normal rates, but grow more slowly than normal and eventually arrest during larval development, reaching only 79% the mass of wild-type controls. The mutants remain viable and active during an extended larval period of about 30 days and eventually die without pupating.

(Zhang et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mTor^EP2353/mTor^k17004 has lethal phenotype, suppressible by S6k^UAS.cWa/Scer\GAL4^Act5C.PP

(Zhang et al., 2000)

mTor^EP2353/mTor^k17004 has lethal phenotype, suppressible by Hsap\RPS6KB1^D4.UAS/Scer\GAL4^Act5C.PP

(Zhang et al., 2000)

mTor^EP2353 has lethal phenotype, suppressible by S6k^UAS.cWa/Scer\GAL4^Act5C.PP

(Zhang et al., 2000)

mTor^EP2353 has lethal phenotype, suppressible by Hsap\RPS6KB1^D4.UAS/Scer\GAL4^Act5C.PP

(Zhang et al., 2000)

NOT Suppressor of

Statement

Reference

Tor[+]/mTor^EP2353 is a non-suppressor of lethal | larval stage phenotype of gig¹⁹³/gig¹⁰⁹

(Zhang et al., 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

mTor^EP2353 is a suppressor of adult head phenotype of Pten¹¹⁷

(Oldham et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Pten^MGH1/Tor^EP2353 somatic clones show a slight decrease in size compared to wild-type. Tor^EP2353/Tor^k17004, S6k^Scer\UAS.cWa flies are slightly smaller than wild-type, but are fertile and develop at a similar rate to wild-type flies.

(Zhang et al., 2000)

Xenogenetic Interactions

Statement

Reference

Hsap\RPS6KB1^D4.Scer\UAS (driven by Scer\GAL4^Act5C.PP) rescues Tor^EP2353/Tor^EP2353 or Tor^EP2353/Tor^k17004 animals to viability.

(Zhang et al., 2000)

Complementation and Rescue Data

Fails to complement

mTor^2L19

(Oldham et al., 2000)

mTor^2L1

(Oldham et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Tor^EP2353

dTOR^P2

(Zhang et al., 2000)

mTor^EP2353

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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