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General Information
Symbol
Dmel\Tor2L1
Species
D. melanogaster
Name
FlyBase ID
FBal0120583
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dTOR2L1
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C13012397T

Amino acid change:

P2293L | Tor-PA; P2294L | Tor-PB

Reported amino acid change:

P2303L

Comment:

Position of mutation on reference sequence inferred by FlyBase curator. Difference between annotated and reported sites of amino acid substitution due to authors reported CDS being 10aa longer.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: P2303L. This mutation is within the highly conserved area of the kinase domain.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Tor2L1/Tor2L19 larvae are fat (increased TAG levels) and small in size.

The number of nuclei in the segmental border muscle is slightly reduced compared to wild type in mutant embryos.

Mutant embryos have thinner muscles than normal and unfused myoblasts are present.

Cells in Tor2L1 mutant clones in the eye are 0.26 times the size of their heterozygous neighbours.

Tissue specific induction of Tor2L1 mutant clones in the head produces flies with reduced head size relative to the body. Tor2L1/Tork17004 mutants have wing discs that are approximately wild-type in size. The size of the nuclei in the endoreplicating salivary glands are smaller than wild-type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

Tor2L1/Tor[+], Tsc1Q87X has lethal phenotype, suppressible | partially by S6k[+]/S6kl-1

NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference

Tor2L1/Tor[+] is a suppressor | partially of lethal | larval stage phenotype of Tor+t9.4, gig193/gig109

Tor2L1/Tor[+] is a suppressor | partially of lethal | larval stage phenotype of gig193/gig109

Tor2L1/Tor[+] is a suppressor | partially of increased cell size | somatic clone phenotype of Tsc129

Tor2L1/Tor[+] is a suppressor | partially of lethal | recessive | larval stage phenotype of Tsc129

Tor2L1/Tor[+] is a suppressor | partially of lethal phenotype of Tsc1Q87X

Tor2L1/Tor[+] is a suppressor | partially of lethal phenotype of S6k[+]/S6kl-1, Tsc1Q87X

Phenotype Manifest In
Suppressed by
Statement
Reference
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

REPTORKO significantly rescues both the metabolic and size phenotypes of Tor2L1/Tor2L19 larvae.

Around 60% of Tor2L1/+; gig109/gig193 flies survive to adulthood, while no gig109/gig193 survive to the pupal stage. The Tor+t9.4 transgene reduces the amount of Tor2L1/+; gig109/gig193 flies that survive to the pupal stage from around 85% to around 65%.

The large size and disorganisation of ommatidia in Scer\GAL4GMR.PF/+; RhebEP50.084/+ flies is partially dominantly suppressed by Tor2L1 (ommatidia are 1.27 times wild-type size, and slightly disorganised).

The first instar larval lethality due to Tsc129 is partially suppressed by heterozygosity for Tor2L1 : 31% (n = 193) of Tor2L1/+ Tsc129/Tsc129 animals survive to the pupal stage. The increased cell size seen in somatic clones of Tsc129 in the eye is partially suppressed in a Tor2L1 heterozygous background: the resulting clone cells are 1.3X wild-type size, compared to 1.9X for Tsc129 somatic clone cells in a wild-type background. Cells in Tor2L1; Tsc129 double mutant somatic clones are approximately 0.25 times the size of their heterozygous neighbours - i.e.- have little or no size difference with Tor2L1 somatic clone cells.

The second instar lethality of homozygous Tsc1Q87X is rescued to adulthood (18.5%) or pupal stages (82.5%) by heterozygosity for Tor2L1. The second instar lethality of homozygous Tsc1Q87X is rescued to adulthood (62%) or pupal stages (93%) by heterozygosity for both S6kl-1 and Tor2L1. The rescued animals are slightly larger than wild-type flies, with overall patterning appearing normal. The rescued females are semi-fertile when crossed to wild-type males, whereas the rescued males are fully fertile when crossed to wild-type females.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (10)