FlyBase Allele Report: Dmel\mTor[2L19]

General Information

Symbol

Dmel\mTor^2L19

Species

D. melanogaster

Name

FlyBase ID

FBal0120584

Feature type

allele

Associated gene

Dmel\mTor

Associated Insertion(s)

Carried in Construct

Also Known As

TOR^2L19

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Oldham et al., 2000)

Progenitor genotype

Cytology

Description

Amino acid replacement: R248term.

(Oldham et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:13,005,725..13,005,725 [+]

Nucleotide change:

C13005725T

Amino acid change:

R248term | mTor-PA; R248term | mTor-PB

Reported amino acid change:

R248term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Oldham et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size | larval stage (with mTor^2L1)

(Tiebe et al., 2015)

decreased cell number | somatic clone - Minute background | third instar larval stage

(Devilliers et al., 2021)

decreased cell size | somatic clone - Minute background | third instar larval stage

(Devilliers et al., 2021)

decreased occurrence of cell division | second instar larval stage

(Sousa-Nunes et al., 2011)

Phenotype Manifest In

head | somatic clone

(Oldham et al., 2000)

larval fat cell | somatic clone - Minute background | third instar larval stage

(Devilliers et al., 2021)

larval neuroblast

(Sousa-Nunes et al., 2011)

Detailed Description

Statement

Reference

Tor^2L19/Tor^2L19 fat body clones in a Minute background, induced during embryogenesis and analyzed at early third instar larval stage, are small and bear smaller cells than controls.

(Devilliers et al., 2021)

Tor^2L19 mutant clones generated in otherwise wild-type eyes are massively under-represented. The adult eyes are almost completely composed of wild-type twin clone tissue.

(Nowak et al., 2013)

Central nervous system neuroblasts of mutant fed early second instar larvae show a reduction in EdU incorporation compared to controls.

(Sousa-Nunes et al., 2011)

Tissue specific induction of Tor^2L19 mutant clones in the head produces flies with reduced head size relative to the body.

(Oldham et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pten¹¹⁷, mTor^2L19 has increased cell growth rate | somatic clone phenotype, enhanceable by ImpL2^UAS.cHa/Scer\GAL4^Act5C.PP

(Nowak et al., 2013)

Suppressed by

Statement

Reference

mTor^2L1/mTor^2L19 has decreased body size | larval stage phenotype, suppressible | partially by REPTOR^KO

(Tiebe et al., 2015)

NOT suppressed by

Statement

Reference

mTor^2L19 has decreased cell size | third instar larval stage | somatic clone - Minute background phenotype, non-suppressible by Rheb^{EP50.084-loxP}/Scer\GAL4^Tub.PU

(Devilliers et al., 2021)

mTor^2L19 has decreased cell size | third instar larval stage | somatic clone - Minute background phenotype, non-suppressible by Pten¹¹⁷/Pten¹¹⁷

(Devilliers et al., 2021)

Enhancer of

Statement

Reference

Tor[+]/mTor^2L19 is an enhancer of visible phenotype of Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^Bx-MS1096

(Coelho et al., 2005)

Suppressor of

Statement

Reference

mTor^2L19 is a suppressor of increased cell growth rate phenotype of Pten¹¹⁷

(Nowak et al., 2013)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

mTor^2L19 has larval fat cell | third instar larval stage | somatic clone - Minute background phenotype, non-suppressible by Rheb^{EP50.084-loxP}/Scer\GAL4^Tub.PU

(Devilliers et al., 2021)

mTor^2L19 has larval fat cell | third instar larval stage | somatic clone - Minute background phenotype, non-suppressible by Pten¹¹⁷/Pten¹¹⁷

(Devilliers et al., 2021)

Enhancer of

Statement

Reference

Tor[+]/mTor^2L19 is an enhancer of wing phenotype of Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^Bx-MS1096

(Coelho et al., 2005)

Suppressor of

Statement

Reference

mTor^2L19 is a suppressor of eye phenotype of Pten¹¹⁷

(Nowak et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

REPTOR^KO significantly rescues both the metabolic and size phenotypes of Tor^2L1/Tor^2L19 larvae.

(Tiebe et al., 2015)

Eyes mutant for Pten¹¹⁷ and Tor^2L19 are reduced to the size of control eyes at both fed and starved conditions, indicating that the overgrowth of Pten¹¹⁷ clones depends on normal TORC1 function.

The generation of Pten¹¹⁷ and Tor^2L19 sister clones results in eyes that are completely composed of Pten mutant tissue. However, these eyes are smaller than those without Tor^2L19 mutant sister clones.

Ubiquitous expression of ImpL2^Scer\UAS.cHa (under the control of Scer\GAL4^Act5C.PP) results in the overgrowth of Pten¹¹⁷ clones, irrespective of whether they neighbor Tor^2L19 mutant or wild-type cells. The overgrowth of Pten¹¹⁷ clones causes a further reduction in body size, as evidenced by decreased shoulder width.

(Nowak et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

mTor^EP2353

(Oldham et al., 2000)

mTor^k17004

(Oldham et al., 2000)

Rescued by

mTor^2L19 is rescued by mTor^+t9.4

(Zhang et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

TOR^2L19

(Tiebe et al., 2015, Nowak et al., 2013)

Tor^2L19

(Devilliers et al., 2021, Kapuria et al., 2012, Sousa-Nunes et al., 2011, Hsieh et al., 2010)

dTOR^2L19

(Radimerski et al., 2002)

mTor^2L19

Name Synonyms

Secondary FlyBase IDs

References (10)

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