Allele Dmel\alph^S-355

General Information
Symbol	Dmel\alph^S-355	Species	D. melanogaster
Name		FlyBase ID	FBal0120640
Feature type	allele	Associated gene	Dmel\alph

Allele class
Mutagen
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: G173S. (Baril and Therrien, 2006)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (Therrien et al., 2000) partially lethal - majority live (with alph^S-331) (Therrien et al., 2000) partially lethal - majority live (with alph^XS-88) (Therrien et al., 2000) viable (Baril and Therrien, 2006) visible (with alph^S-331) (Therrien et al., 2000) visible (with alph^XS-88) (Therrien et al., 2000)
Phenotype Manifest In
	eye (with alph^S-331) (Therrien et al., 2000) eye (with alph^XS-88) (Therrien et al., 2000) wing (with alph^S-331) (Therrien et al., 2000) wing (with alph^XS-88) (Therrien et al., 2000) wing vein (with alph^S-331) (Therrien et al., 2000) wing vein (with alph^XS-88) (Therrien et al., 2000)
Detailed Description
	Statement Reference Heterozygotes with pp2c99B^XS-88 or pp2c99B^S-331 are sub-viable with rough eyes and defects including missing wing veins and atrophied wings. (Therrien et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference alph^S-355/pp2c99B[+] is an enhancer of visible phenotype of Ras85D^V12.sev (Therrien et al., 2000)
Suppressor of
Statement Reference alph^S-355/pp2c99B[+] is a suppressor of lethal phenotype of phl¹² (Therrien et al., 2000) alph^S-355/pp2c99B[+] is a suppressor of visible phenotype of ksr::tor^{ΔNksr.hs.sev} (Therrien et al., 2000)
Phenotype Manifest In
Enhancer of
Statement Reference alph^S-355/pp2c99B[+] is an enhancer of eye phenotype of Ras85D^V12.sev (Therrien et al., 2000) alph^S-355 is an enhancer of eye phenotype of Ras85D^V12.sev (Baril and Therrien, 2006) alph^S-355 is an enhancer of photoreceptor cell R7 \| supernumerary phenotype of rl^Sem.sev (Baril and Therrien, 2006)
NOT Enhancer of
Statement Reference alph^S-355 is a non-enhancer of eye phenotype of phyl^hs.sev (Baril and Therrien, 2006)
Suppressor of
Statement Reference alph^S-355/pp2c99B[+] is a suppressor of eye phenotype of ksr::tor^{ΔNksr.hs.sev} (Therrien et al., 2000) alph^S-355 is a suppressor of photoreceptor cell phenotype of ksr::tor^{ΔNksr.hs.sev} (Baril and Therrien, 2006)
Additional Comments
Genetic Interactions
Statement Reference rl^Sem.sev heterozygous mutants in combination with alph^S-355 exhibit a rough eye phenotype. They exhibit an average of 3.39 R7 cells per ommatidium, compared to 1 R7 cell per ommatidium in wild-type. ksr::tor^{ΔNksr.hs.sev}/alph^S-355 mutants exhibit a 20% loss of ommatidial R7 cells, indicating that alph^S-355 has suppressed the ksr::tor^{ΔNksr.hs.sev} phenotype. This effect is not specific to the R7 cells as the loss of outer photoreceptors is suppressed in this double mutant. The rough eye phenotype of Ras85D^V12.sev mutants is increased when transheterozygous with alph^S-355. phyl^hs.sev heterozygous mutants in combination with alph^S-355 exhibit a rough eye phenotype. They exhibit an average of 1.70 R7 cells per ommatidium, compared to 1 R7 cell per ommatidium in wild-type. This however is no higher than in phyl^hs.sev heterozygous mutants. (Baril and Therrien, 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	alph^S-331 (Therrien et al., 2000) alph^XS-88 (Therrien et al., 2000)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	alph^S-355 pp2c99B^S-355 S-355 (Baril and Therrien, 2006) SK3-1^S-355 (Therrien et al., 2000, )
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Baril and Therrien, 2006, Dev. Biol. 294(1): 232--245 Alphabet, a Ser/Thr phosphatase of the protein phosphatase 2C family, negatively regulates RAS/MAPK signaling in Drosophila. [FBrf0190087] Therrien et al., 2000, Genetics 156(3): 1231--1242 A genetic screen for modifiers of a kinase suppressor of ras-dependent rough eye phenotype in Drosophila. [FBrf0131405]

Allele Dmel\alphS-355

Allele Dmel\alph^S-355