FlyBase Allele Report: Dmel\S6k[UAS.cWa]

General Information

Symbol

Dmel\S6k^UAS.cWa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Watson

FlyBase ID

FBal0120644

Feature type

allele

Associated gene

Dmel\S6k

Associated Insertion(s)

Carried in Construct

P{UAS-S6k.W}

Also Known As

UAS-S6K, UAS-dS6k

Key Links

Transgenic product class

wild_type

(Zhang et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Zhang et al., 2000)

Mutagen

in vitro construct

(Zhang et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-S6k.W}

Cytology

Description

UAS regulatory sequences drive expression of S6k.

(Zhang et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

S6k

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^GMR.PF

(Xu et al., 2008)

chemical resistant, with Scer\GAL4^Act5C.PP

(Zhang et al., 2000)

visible, with Scer\GAL4^ap-md544

(Lee and Chung, 2007)

visible | adult stage, with Scer\GAL4^GMR.PF

(Xu et al., 2008)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Xu et al., 2008, Goberdhan et al., 2005)

wing, with Scer\GAL4^ap-md544

(Lee and Chung, 2007)

Detailed Description

Statement

Reference

Expression of S6k^Scer\UAS.cWa under the control of Scer\GAL4^GMR.PF results in eyes that are slightly larger than wild type.

(Xu et al., 2008)

Expression of S6k^Scer\UAS.cWa under the control of Scer\GAL4^ap-md544 results in wings which are bent down due to overgrowth of the dorsal part.

(Lee and Chung, 2007)

Expression of S6k^Scer\UAS.cWa, under the control of Scer\GAL4^GMR.PF, promotes modest overgrowth of the eye.

(Goberdhan et al., 2005)

Expression of S6k^Scer\UAS.cWa under the control of Scer\GAL4^ey.PH has no effect on eye size.

(Tapon et al., 2001)

When S6k^Scer\UAS.cWa is driven by Scer\GAL4^Act5C.PP in wild-type flies a significant resistance to rapamycin is conferred.

(Zhang et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

S6k^UAS.cWa, Scer\GAL4^GMR.PF has visible | adult stage phenotype, suppressible | partially by RpL8^RNAi.UAS, Scer\GAL4^GMR.PF

(Xu et al., 2008)

S6k^UAS.cWa, Scer\GAL4^GMR.PF has abnormal size | adult stage phenotype, suppressible | partially by RpL8^RNAi.UAS, Scer\GAL4^GMR.PF

(Xu et al., 2008)

S6k^UAS.cWa, Scer\GAL4^GMR.PF has visible | adult stage phenotype, suppressible | partially by Hsap\RPL8^DN.UAS, Scer\GAL4^GMR.PF

(Xu et al., 2008)

S6k^UAS.cWa, Scer\GAL4^GMR.PF has abnormal size | adult stage phenotype, suppressible | partially by Hsap\RPL8^DN.UAS, Scer\GAL4^GMR.PF

(Xu et al., 2008)

S6k^UAS.cWa, Scer\GAL4^ap-md544 has visible phenotype, suppressible by raptor^RNAi.Sym.UAS, Scer\GAL4^ap-md544

(Lee and Chung, 2007)

NOT suppressed by

Statement

Reference

S6k^UAS.cWa, Scer\GAL4^ap-md544 has visible phenotype, non-suppressible by rictor^Δ24

(Lee and Chung, 2007)

S6k^UAS.cWa, Scer\GAL4^ap-md544 has visible phenotype, non-suppressible by rictor^Δ42

(Lee and Chung, 2007)

Suppressor of

Statement

Reference

S6k^UAS.cWa, Scer\GAL4^ap-md544 is a suppressor of visible phenotype of Scer\GAL4^ap-md544, raptor^RNAi.Sym.UAS

(Lee and Chung, 2007)

S6k^UAS.cWa, Scer\GAL4^ey.PH is a suppressor of abnormal size | adult stage phenotype of Scer\GAL4^ey.PH, Tsc1^UAS.cTa, gig^UAS.cTa

(Tapon et al., 2001)

S6k^UAS.cWa, Scer\GAL4^ey.PH is a suppressor of visible phenotype of Scer\GAL4^ey.PH, Tsc1^UAS.cTa, gig^UAS.cTa

(Tapon et al., 2001)

S6k^UAS.cWa/Scer\GAL4^Act5C.PP is a suppressor of lethal phenotype of mTor^EP2353

(Zhang et al., 2000)

S6k^UAS.cWa/Scer\GAL4^Act5C.PP is a suppressor of lethal phenotype of mTor^EP2353/mTor^k17004

(Zhang et al., 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

S6k^UAS.cWa, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by Hsap\RPL8^DN.UAS, Scer\GAL4^GMR.PF

(Xu et al., 2008)

S6k^UAS.cWa, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by RpL8^RNAi.UAS, Scer\GAL4^GMR.PF

(Xu et al., 2008)

S6k^UAS.cWa, Scer\GAL4^ap-md544 has wing phenotype, suppressible by raptor^RNAi.Sym.UAS, Scer\GAL4^ap-md544

(Lee and Chung, 2007)

NOT suppressed by

Statement

Reference

S6k^UAS.cWa, Scer\GAL4^ap-md544 has wing phenotype, non-suppressible by rictor^Δ24

(Lee and Chung, 2007)

S6k^UAS.cWa, Scer\GAL4^ap-md544 has wing phenotype, non-suppressible by rictor^Δ42

(Lee and Chung, 2007)

Enhancer of

Statement

Reference

S6k^UAS.cWa, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, foxo^GS9928

(Goberdhan et al., 2005)

Suppressor of

Statement

Reference

S6k^UAS.cWa, Scer\GAL4^ap-md544 is a suppressor of wing phenotype of Scer\GAL4^ap-md544, raptor^RNAi.Sym.UAS

(Lee and Chung, 2007)

S6k^UAS.cWa, Scer\GAL4^ey.PH is a suppressor of eye phenotype of Scer\GAL4^ey.PH, Tsc1^UAS.cTa, gig^UAS.cTa

(Tapon et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of RpL8^{dsRNA.Scer\UAS} partially suppresses the increase in eye size seen when S6k^Scer\UAS.cWa is expressed under the control of Scer\GAL4^GMR.PF.

(Xu et al., 2008)

The wing phenotype caused by expression of S6k^Scer\UAS.cWa under the control of Scer\GAL4^ap-md544 is not altered in a rictor^Δ24 or rictor^Δ42 background.

Flies co-expressing both S6k^Scer\UAS.cWa and raptor^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^ap-md544 have normal wings.

(Lee and Chung, 2007)

Co-expression of S6k^Scer\UAS.cWa strongly enhances the Scer\GAL4^GMR.PF>foxo^GS9928 ommatidial phenotype.

(Goberdhan et al., 2005)

The small ommatidium size caused by coexpression of gig^Scer\UAS.cTa and Tsc1^Scer\UAS.cPa under the control of Scer\GAL4^GMR.PF is suppressed by coexpression of S6k^Scer\UAS.cWa.

(Potter et al., 2001)

Expression of both Tsc1^Scer\UAS.cTa and gig^Scer\UAS.cTa in the eye under the control of Scer\GAL4^ey.PH results in a much smaller eye than normal. Coexpression of S6k^Scer\UAS.cWa suppresses the phenotype.

(Tapon et al., 2001)

The addition of S6k^Scer\UAS.cWa (driven by Scer\GAL4^Act5C.PP) to Tor^EP2353/Tor^EP2353 or Tor^EP2353/Tor^k17004 animals, rescues the Tor phenotype to viability.

(Zhang et al., 2000)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\RPL8^DN.Scer\UAS partially suppresses the increase in eye size seen when S6k^Scer\UAS.cWa is expressed under the control of Scer\GAL4^GMR.PF.

(Xu et al., 2008)

Complementation and Rescue Data

Rescues

S6k^UAS.cWa/Scer\GAL4^Act5C.PP rescues S6k^l-1

(Kockel et al., 2010)

Comments

Expression of S6k^Scer\UAS.cWa under the control of Scer\GAL4^Act5C.PP rescues the lethality associated with S6k^l-1.

(Kockel et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

S6k^Scer\UAS.cWa

S6k^UAS.cWa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Watson

(Zhang et al., 2000)

Secondary FlyBase IDs

References (8)

Report Sections

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