Open Close
General Information
Symbol
Dmel\Rab5S43N.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0120656
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Rab5DN, Rab5DN, Rab5SN, UAS-Rab5S43N, UAS-Rab5.S43N, UAS-DRab5S43N, UAS-Rab5S43N, Rab5S43N, UAS-Rab5-DN
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS regulatory sequences drive expression of a dominant negative form of Rab5.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
egg chamber & basement membrane | apical, with Scer\GAL4slbo.2.6
synaptic vesicle & larval neuromuscular junction, with Scer\GAL4elav-C155
Detailed Description
Statement
Reference
Adults expressing Rab5S43N.UAS under the control of Scer\GAL4elav.PLu do not exhibit climbing defects compared to control
Expressing Rab5S43N.UAS under the control of Scer\GAL4ppk.PU induces enlarged endosomes but does not affect mitochondria localization in white prepupal ddaC neurons' cell bodies, as compared to controls.
Scer\GAL4He.PZ-driven expression of Rab5S43N.Scer\UAS induces increase in the number of circulating hemocytes in third instar larvae relative to controls.
The third instar larval neuromuscular junction of individuals expressing Rab5S43N.Scer\UAS under the control of Scer\GAL4da.G32 exhibits the accumulation of large presynaptic endocytic vesicles, as compared to controls.
Animals expressing Rab5S43N.Scer\UAS under the control of Scer\GAL4grh.D4 are lethal, show defects in larval dorsal trunk elongation (resulting in internalization of posterior spiracles into the body cavity) and the larvae are smaller than age-matched controls.
Expression of Rab5S43N.Scer\UAS under the control of Scer\GAL4SPARC-MI00329-GAL4 results in elongation of the ventral nerve cord. Third larval instar adipocytes expressing Rab5S43N.Scer\UAS under the control of Scer\GAL4SPARC-MI00329-GAL4 show a significant increase in plasma membrane depth compared to control cells.
The class IV dendritic arborization neurons in flies expressing Rab5S43N.Scer\UAS under the control of Scer\GAL4ppk.PG show a significant decrease in the complexity of dendritic arbors.
Third instar larvae expressing Rab5S43N.Scer\UAS under the control of Scer\GAL4VGlut-OK371 show a significant increase in the number of boutons per muscle area at the neuromuscular junction (NMJ) compared to controls and the boutons are smaller than normal. Class IV da neurons show mild defects in dendrite morphogenesis: total dendritic length is decreased by 11% and the number of branch endings per cell is decreased by 19%, while the coverage index is not altered. Adults expressing Rab5S43N.Scer\UAS under the control of Scer\GAL4VGlut-OK371 show a significant increase in the number of boutons per muscle area at the neuromuscular junction (NMJ) compared to controls.
Expression of Rab5S43N.Scer\UAS under the control of Scer\GAL4da.G32 affects dorsal closure. The amnioserosa cells are irregular in shape with big lamellipodia during the early stages of dorsal closure. Later on, epidermal elongation is impaired and puckering is seen. Dorsal closure fails, with the embryos having a conspicuous dorsal hole in the cuticle. Expression of Rab5S43N.Scer\UAS under the control of Scer\GAL4c381 results in changes in amnioserosa cell morphology during early and late stages of dorsal closure, but epidermal cell elongation is not compromised and the embryos complete dorsal closure. Expression of Rab5S43N.Scer\UAS under the control of Scer\GAL4en-e16E has no visible effect on dorsal closure.
Third-instar larval eye discs expressing dominant negative Rab5S43N.Scer\UAS in the posterior region of the under control of Scer\GAL4GMR.PF display normal morphology, with differentiating R-cells in the eye disc expressing normal R-cell developmental markers. However, many R-cell nuclei fail to maintain their apical localization and instead mis-localize basally.
Embryos expressing Rab5S43N.Scer\UAS under the control of Scer\GAL469B demonstrate over-elongated dorsal trunks.
Mutant neurons in the third instar larva expressing Rab5S43N.Scer\UAS show reduced branch numbers and sprout much less in the proximal area than wild-type neurons. Neurons expressing Rab5S43N.Scer\UAS extend axons and major dendritic branches, however, they display greatly simplified dendritic arbors and decreased number of terminal branches compared with that found for wild-type neurons.
No third instar larvae could be recovered that express Rab5S43N.Scer\UAS under the control of Scer\GAL4en-e16E. Viable larvae can be obtained when Rab5S43N.Scer\UAS is expressed under the control of Scer\GAL4C5. The wing discs of these larvae contain misfolded tissue that contains apoptotic cells. Expression of Rab5S43N.Scer\UAS under the control of Scer\GAL4Bx-MS1096 or Scer\GAL4C96 also causes apoptosis in the wing disc. A few clones expressing Rab5S43N.Scer\UAS under the control of Scer\GAL4Act5C.PI can be recovered in the wing pouch of wandering third instar larvae, although these become extruded from the epithelium or are lost through cell death.
Overexpression of Rab5S43N.Scer\UAS under the control of Scer\GAL4bbg-C96 results in the loss of wing tissue. Overexpression of Rab5S43N.Scer\UAS under the control of Scer\GAL4C5 results almost complete loss of wing tissue.
Expression of Rab5S43N.Scer\UAS in border cells, driven by Scer\GAL4slbo.2.6, results in the absence of the apical cap in stage 8 egg chambers, as compared with wild type. Among clusters still showing a cap, 47% show a micro cap. The migration of border cell clusters to the oocyte is defective.
Blocking endocytosis in border cells by expressing Rab5S43N.Scer\UAS under the control of Scer\GAL4slbo.2.6 delays the migration of these cells.
The average number of crystal cells per embryo is reduced compared to wild type in stage 13-14 embryos expressing Rab5S43N.Scer\UAS under the control of Scer\GAL4srp.
Expression of dominant-negative Rab5S43N.Scer\UAS under the regulation of Scer\GAL4elav-C155 at low levels during embryonic and early larval stages at 16oC and at higher levels at 25oC during the last two days of larval development does not cause a developmental phenotype of the neuromuscular junctions. The synaptic surface area is normal in Rab5S43N.Scer\UAS-expressing synapses. In addition, Rab5S43N.Scer\UAS-expressing motoneurons display a normal number and morphology of endocytosis centers and active zones of exocytosis. In Rab5S43N.Scer\UAS synapses (under the regulation of Scer\GAL4elav-C155) small, 70nm vesicles are more frequently observed than in wild-type, indicating that these endocytic vesicles are unable to fuse efficiently with early endosomes. In wild-type and Rab5S43N.Scer\UAS (Scer\GAL4elav-C155) synapses, FM1-43 styryl dye recycling experiments reveal that the rate of FM1-43 internalisation is 2.5-fold slower in Rab5S43N.Scer\UAS synapses compared to wild-type. Synapse release is also affected in Rab5S43N.Scer\UAS mutants. FM1-43-release experiments reveal that release occurs 2.5 times slower during the first 5min in Rab5S43N.Scer\UAS synapses compared to wild-type, indicating that impaired Rab5 function reduces small vesicle release. Miniature excitatory junction potential (mEJP) recordings from Rab5S43N.Scer\UAS mutant neuromuscular junctions display no significant differences in mean frequency, amplitude, variability, or voltage decay kinetics compared to wild-type mEJPs. This indicates that presynaptic expression of Rab5S43N.Scer\UAS does not affect the vesicular neurotransmitter content, the number of fusion competent vesicles, or the postsynaptic glutamate receptor function or density. Consistently, a normal number of docked vesicles is observed at the ultra-structural level, and no difference in the overall morphology, synaptic surface area, or active zones is detected. Mean quantal content decreases by around 50% in Rab5S43N.Scer\UAS; Scer\GAL4elav-C155 neuromuscular junctions. Rab5S43N.Scer\UAS, under the regulation of Scer\GAL4elav-C155 does not significantly alter the synaptic area, nor the active zone density of presynaptic terminals, implying that the total number of active zones is normal.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Suppressor of
Statement
Reference
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
OcrlΔ3 mutants also expressing Rab5S43N.Scer\UAS under the control of Scer\GAL4He.PZ are early larval lethal.
The number of oocytes containing abnormal large vesicles observed upon DCTN2-p50dsRNA.A.Scer\UAS.P\T-driven expression of Scer\GAL4mat.αTub67C.T:Hsim\VP16 is significantly reduced by co-expression of Rab5S43N.Scer\UAS.
Expression of Rab5S43N.Scer\UAS under the control of Scer\GAL4Act5C.PI in scrib1 somatic mutant clones results in aggressive overgrowth of eye-antennal disc mutant tissue.
Xenogenetic Interactions
Statement
Reference
The reduced number of boutons on neuromuscular junctions observed in third instar larvae expressing Hsap\TARDBPScer\UAS.cHa under the Scer\GAL4VGlut.PD driver cannot be restored by co-expression of Rab5S43N.Scer\UAS.
The formation of giant autophagic bodies in the retina of adult flies expressing Hsap\MAPTGMR.Ex.PJ is partially suppressed by co-expression of Rab5S43N.Scer\UAS under the control of Scer\GAL4GMR.PU.
Co-expression of Rab5S43N.Scer\UAS and Mmus\PkacamC.Scer\UAS under the control of Scer\GAL4ppk.PG results in more severe loss of complexity in class IV dendritic arbors than when either transgene is expressed alone.
Coexpression of BacA\p35Scer\UAS.cHa with Rab5S43N.Scer\UAS, under the control of Scer\GAL4Act5C.PI in clones of the wing disc show better survival than clones expressing only Rab5S43N.Scer\UAS, but the surviving clones still become extruded from the epithelium, as with clones that only express Rab5S43N.Scer\UAS.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (43)