Allele Dmel\Phm^P(29)

General Information
Symbol	Dmel\Phm^P(29)	Species	D. melanogaster
Name		FlyBase ID	FBal0120693
Feature type	allele	Associated gene	Dmel\Phm

Allele class	loss of function allele
Mutagen	Delta2-3
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Jiang et al., 2000)
Mutagen	Delta2-3 (Jiang et al., 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Phm^k07623 (Jiang et al., 2000)
Nature of the lesion	Statement Reference Imprecise excision of the Phm^k07623 insertion has produced a deletion that removes 1309 bp of Phm genomic sequence. About 0.4kb of the original P{lacW} insertion also remains. (Jiang et al., 2000)
Caused by aberration	Df(2R)Phm^P(29) (Jiang et al., 2000)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (Jiang et al., 2000)
Phenotype Manifest In
	pupa, with Phm^A5.hs (Jiang et al., 2000) puparium, with Phm^A5.hs (Jiang et al., 2000)
Detailed Description
	Statement Reference With very few exceptions homozygotes fail to hatch, those that do are nearly immobile and die within a few hours. Although failing to hatch, most embryos complete the large part of embryogenesis prior to their death. Little evidence for tissue disorganisation or morphological abnormality is seen. The G2 progeny of Phm^P(29) adults rescued by Phm^A5.hs have apparently normal central nervous systems (CNSs) about 3/4 of these embryos complete embryogenesis and hatch, about 3/5 reach pupal stages and about 1/5 complete metamorphosis. Puparium formation is often aberrant: puparia typically display a slight curvature, a failure to shorten normally, and a failure to evert anterior spiracles properly. The gas bubble fails to move anteriorly, leaving pupae in anterior position with abnormal posterior gas spaces. The majority of these non eclosing puparia die after completing pupation and proper eversion of the head wing and legs, but reveal no further adult differentiation, dying between stages P4ii and P7. Many animals also reaching the same stage display aberrant disc eversion, retention of larval mouthparts and imprecise elimination of apolysed tracheal cuticle. Almost none of the prepupae that fail to eclose differentiate past stage P7. Daily heatshock inductions do not increase the survival of these G2 animals. (Jiang et al., 2000)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Phm^k07623/Phm^P(29) is rescued by Scer\GAL4^dimm-929/Scer\GAL4^386Y/Phm^Scer\UAS.cTA (Taghert et al., 2001) Phm^k07623/Phm^P(29) is rescued by Scer\GAL4^dimm-929/Scer\GAL4^Appl.G1a/Phm^Scer\UAS.cTA (Taghert et al., 2001) Phm^k07623/Phm^P(29) is rescued by Scer\GAL4^elav-C155/Scer\GAL4^dimm-929/Phm^Scer\UAS.cTA (Taghert et al., 2001) Phm^k07623/Phm^P(29) is rescued by Scer\GAL4^tim.PE/Phm^Scer\UAS.cTA/Scer\GAL4^Kdm2-36Y (Taghert et al., 2001) Phm^k07623/Phm^P(29) is rescued by Scer\GAL4^tim.PE/Scer\GAL4^dimm-929/Phm^Scer\UAS.cTA (Taghert et al., 2001)
Partially rescued by	Phm^k07623/Phm^P(29) is partially rescued by Phm^Scer\UAS.cTA/Scer\GAL4^Kdm2-36Y (Taghert et al., 2001) Phm^k07623/Phm^P(29) is partially rescued by Scer\GAL4^386Y/Phm^Scer\UAS.cTA (Taghert et al., 2001) Phm^k07623/Phm^P(29) is partially rescued by Scer\GAL4^dimm-929/Phm^Scer\UAS.cTA (Taghert et al., 2001) Phm^k07623/Phm^P(29) is partially rescued by Scer\GAL4^dimm-929/Phm^Scer\UAS.cTA/Scer\GAL4^Kdm2-36Y (Taghert et al., 2001) Phm^k07623/Phm^P(29) is partially rescued by Scer\GAL4^dimm-929/Scer\GAL4^D42/Phm^Scer\UAS.cTA (Taghert et al., 2001) Phm^k07623/Phm^P(29) is partially rescued by Scer\GAL4^dimm-929/Scer\GAL4^P2.4.Pdf/Phm^Scer\UAS.cTA (Taghert et al., 2001) Phm^P(29) is partially rescued by Phm^A5.hs (Jiang et al., 2000) Phm^P(29) is partially rescued by Scer\GAL4^dimm-929/Phm^Scer\UAS.cTA (Taghert et al., 2001)
Not rescued by	Phm^k07623/Phm^P(29) is not rescued by Scer\GAL4^tim.PE/Phm^Scer\UAS.cTA (Taghert et al., 2001)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	PHM⁰² (Taghert et al., 2001) PHM^P29 (Taghert et al., 2001) Phm^P(29)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Taghert et al., 2001, J. Neurosci. 21(17): 6673--6686 Multiple amidated neuropeptides are required for normal circadian locomotor rhythms in Drosophila. [FBrf0138448] Jiang et al., 2000, Dev. Biol. 226(1): 118--136 PHM is required for normal developmental transitions and for biosynthesis of secretory peptides in Drosophila. [FBrf0129874]

Allele Dmel\PhmP(29)

Allele Dmel\Phm^P(29)