FlyBase Allele Report: Dmel\Dredd[B118]

General Information

Symbol

Dmel\Dredd^B118

Species

D. melanogaster

Name

FlyBase ID

FBal0120966

Feature type

allele

Associated gene

Dmel\Dredd

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Leulier et al., 2000)

Mutagen

ethyl methanesulfonate

(Leulier et al., 2000)

Progenitor genotype

Cytology

Description

Amino acid replacement: R127term.

(Leulier et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:635,067..635,067 [-]

Nucleotide change:

C635067T

Amino acid change:

R105term | Dredd-PD; R105term | Dredd-PE; R105term | Dredd-PF; R51term | Dredd-PG

Reported amino acid change:

R127term

Comment:

The predicted amino acid change was reported relative to a 516aa Dredd protein, which would be created by the use of an uptream translation start. The current Dredd genome annotation uses a downstream AUG which is supported by both species conservation and RNA-seq data. The two start sites are 22 aa apart.

(Leulier, 2019.9.22, Leulier et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates retinal disease

modeled by norpA^EE5

(Chinchore et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

intestinal stem cell

(Wang et al., 2013)

retina | conditional

(Chinchore et al., 2012)

rhabdomere | conditional

(Chinchore et al., 2012)

spermatid

(Huh et al., 2004)

Detailed Description

Statement

Reference

As measured by H[[2]]O[[2]] ingestion, Dredd^B118 mutants are more resistant to oxidative stress than wild-type.

In the absence of infection, Dredd^B118 mutants have rates of intestinal stem cell division that is at least three time as high as those of control flies.

There is no significant difference in bacterial load after infection with Vibrio cholerae between control and mutant flies.

(Wang et al., 2013)

Dredd^B118 mutant flies upon continuous light exposure for 6 days show visible changes in retinal morphology (increase in intraommatidial space, reduced rhabdomeres size, and loss of rhabdomeres in some photoreceptors) while no retinal morphological changes are seen in dark-raised flies as compared to WT control.

(Chinchore et al., 2012)

Mutant flies show greatly reduced survival compared to wild type after septic injury with Erwinia carotovora carotovora 15.

(Meinander et al., 2012)

Dredd^B118 homozygotes exhibit wild-type apoptosis of vCrz neurons, with no viable vCrz neurons detected at 7 hours after puparium formation.

(Lee et al., 2011)

Homozygous and hemizygous flies are resistant to V. cholerae infection compared to controls.

24 hours after infection with V. cholerae, the bacterial load of homozygous mutants is comparable to or greater than controls - resistance is not the result of decreased colonization.

Homozygous mutants are slightly more susceptible to infection by V. cholerae by septic injury compared to controls.

24 hours after infection with V. cholerae, homozygous mutants show increased apoptosis in the intestine compared to controls.

(Berkey et al., 2009)

Developmental apoptosis is normal in embryos that are maternally and zygotically mutant for Dredd^B118.

Developmental apoptosis is normal in homozygous third instar eye discs.

γ-ray induced apoptosis is not suppressed in homozygous third instar wing discs.

(Kondo et al., 2006)

High mortality levels are observed when Dredd^B118 flies are fed on the ROS-resistant KNU53775 yeast strain but are not observed when they are fed on a standard yeast strain (W303). Wild-type flies do not show the same sensitivity to the KNU53775 strain. The number of KNU53775 microbes is ~100 times higher in the intestines of Dredd^B118 flies than in controls.

Dredd^B118 flies show increased mortality following infection with a Salmonella strain that overexpresses an antioxidant gene, but these flies show no increased mortality following infection with the same Salmonella stain but without antioxidant overexpression. There is a marked microbial persistance of oxidant-overexpressing Salmonella in the intestines of Dredd^B118 flies compared to controls.

(Ryu et al., 2006)

Mutant flies show similar survival rates as control flies following natural infection (feeding with contaminated food) with either "Ecc-15" (P.carotovorum.carotovorum), S.cerevisiae or M.luteus.

(Ha et al., 2005)

Dredd^B118 flies show a normal survival rate after natural infection with "Ecc15" (P.carotovorum.carotovorum).

(Ha et al., 2005)

Defects are seen in the movement of investment cones in mutant spermatid, which normally move tailwards in a co-ordinated fashion. In about half of cysts from mutant testes display defects in individualisation. In other cysts individually occurs apparently normal.

(Huh et al., 2004)

Mutant animals have an increased susceptibility to E.coli infection. 30 and 40% of homozygous and heterozygous mutants survive respectively.

(Di Fruscio et al., 2003)

Mutant flies are more susceptible to infection by the Gram negative bacterium Erwinia carotovora than controls.

(Leulier et al., 2003)

Mutant animals are highly susceptible to Gram-negative bacterial infection, exhibiting a dramatic loss of survival rate after infection, with all animals dying within 3 days.

(Leulier et al., 2002)

Mutant adults are highly susceptible to infection by E.coli (survival rate is reduced after pricking with an infected needle compared to survival rate of control flies). Mutant adults are not highly susceptible to infection by M.luteus.

(Leulier et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Dredd^B118 has abnormal immune response phenotype, non-enhanceable by Scer\GAL4^da.G32/pll^RNAi.UAS

(Ryu et al., 2006)

Suppressed by

Statement

Reference

Dredd^B118 has abnormal immune response phenotype, suppressible by CecA1^UAS.cRa/Scer\GAL4^cad-em459

(Ryu et al., 2006)

NOT Enhancer of

Statement

Reference

Dredd^B118 is a non-enhancer of abnormal cell death phenotype of Dronc^I24, Strica⁴

(Lee et al., 2011)

Dredd^B118/Dredd[+] is a non-enhancer of visible phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Dredd^B118 is a non-enhancer of abnormal immune response phenotype of Irc^RNAi.UAS, Scer\GAL4^da.G32

(Ha et al., 2005)

Suppressor of

Statement

Reference

Dredd^B118 is a suppressor of abnormal neuroanatomy | adult stage | progressive | conditional phenotype of norpA^EE5

(Chinchore et al., 2012)

Dredd^B118 is a suppressor of partially lethal - majority die phenotype of PGRP-LF²⁰⁰

(Maillet et al., 2008)

Dredd^B118 is a suppressor of decreased cell death phenotype of rpr^GMR.PH

(Di Fruscio et al., 2003)

NOT Suppressor of

Statement

Reference

Dredd^B118/Dredd^B118 is a non-suppressor of FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone phenotype of M(2)56F^H

(Kale et al., 2015)

Dredd^B118/Dredd^B118 is a non-suppressor of increased cell death | somatic clone phenotype of M(2)56F^H

(Kale et al., 2015)

Dredd^B118 is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of fzy¹/fzy⁵⁰³²

(Kuang et al., 2014)

Dredd^B118 is a non-suppressor of decreased cell number | larval stage phenotype of fzy¹/fzy⁵⁰³²

(Kuang et al., 2014)

Dredd^B118 is a non-suppressor of abnormal cell death phenotype of Dronc^I24, Strica⁴

(Lee et al., 2011)

Dredd^B118 is a non-suppressor of visible phenotype of AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH

(Joza et al., 2008)

Dredd^B118 is a non-suppressor of visible | adult stage phenotype of PGRP-LF²⁰⁰

(Maillet et al., 2008)

Dredd^B118/Dredd[+] is a non-suppressor of visible phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Dredd^B118 is a non-suppressor of visible phenotype of hid^GMR.PG

(Kondo et al., 2006)

Other

Statement

Reference

Dredd^B118/Dredd[+], Strica⁴ has abnormal neuroanatomy | adult stage phenotype

(Lee et al., 2013)

Dredd^B118/Dredd[+], Strica⁴ has decreased cell death | adult stage phenotype

(Lee et al., 2013)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Dredd^B118 is a non-enhancer of neuron phenotype of Dronc^I24, Strica⁴

(Lee et al., 2011)

Dredd^B118/Dredd[+] is a non-enhancer of microchaeta phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Dredd^B118/Dredd[+] is a non-enhancer of macrochaeta phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Suppressor of

Statement

Reference

Dredd^B118 is a suppressor of retina | progressive | conditional phenotype of norpA^EE5

(Chinchore et al., 2012)

Dredd^B118 is a suppressor of eye phenotype of rpr^GMR.PH

(Di Fruscio et al., 2003)

NOT Suppressor of

Statement

Reference

Dredd^B118 is a non-suppressor of neuroblast | larval stage phenotype of fzy¹/fzy⁵⁰³²

(Kuang et al., 2014)

Dredd^B118 is a non-suppressor of neuron phenotype of Dronc^I24, Strica⁴

(Lee et al., 2011)

Dredd^B118 is a non-suppressor of eye phenotype of AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH

(Joza et al., 2008)

Dredd^B118 is a non-suppressor of wing phenotype of PGRP-LF²⁰⁰

(Maillet et al., 2008)

Dredd^B118/Dredd[+] is a non-suppressor of microchaeta phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Dredd^B118/Dredd[+] is a non-suppressor of macrochaeta phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Dredd^B118 is a non-suppressor of eye phenotype of hid^GMR.PG

(Kondo et al., 2006)

Dredd^B118/Dredd^B118 is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Moreno et al., 2002)

Other

Statement

Reference

Dredd^B118/Dredd[+], Strica⁴ has peptidergic neuron | ectopic | adult stage phenotype

(Lee et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The apoptosis that occurs at the interface of wild type and M(2)56F^H/+ cells in M(2)56F^H/+ wing discs in which wild type clones have been induced is not significantly lowered in discs that are also homozygous for Dredd^B118.

(Kale et al., 2015)

Dredd^B118 does not suppress the neuroblast loss seen in fzy⁵⁰³²/fzy¹ mutant larval brains.

(Kuang et al., 2014)

The programmed cell death of bursCCAP neurons seen in the adult ventral nerve cord of wild type flies after eclosion is strongly suppressed in dream⁴/Dredd^B118 mutants.

(Lee et al., 2013)

Triple mutants of Dredd^B118; dream⁴; Nc^I24 display results comparable to dream⁴; Nc^I24 double mutants, i.e. all vCrz neurons are still present having not undergone apoptosis by 7 hours after puparium formation.

(Lee et al., 2011)

The mutant eye phenotype caused by expression of AIF^{ΔN.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH is not ameliorated by Dredd^B118/Y.

(Joza et al., 2008)

Dredd^B118 does not suppress the notching phenotypes and low adult viability seen in homozygous PGRP-LF²⁰⁰ mutants.

(Maillet et al., 2008)

Dredd^B118 dream⁴ double mutant females do not show any defects during oogenesis.

(Baum et al., 2007)

Dredd^B118; pll^{dsRNA.Scer\UAS}/+; Scer\GAL4^da.G32/+ flies show similar immune response susceptibility to ROS-resistent yeast infection to Dredd^B118 single mutant flies.

Expression of CecA1^Scer\UAS.cRa, under the control of Scer\GAL4^cad-em459, ameliorates the survival rates and high intestinal microbial titers of Dredd^B118 flies in response to the ROS-resistant KNU53775 yeast strain and Salmonella overexpressing an antioxidant gene.

(Ryu et al., 2006)

Homozygosity for Dredd^B118 does not supress the ablation of adult eye tissue seen in egr^Scer\UAS.cMa; Scer\GAL4^GMR.PF animals.

(Moreno et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

B118

(Leulier, 2019.9.22, Leulier et al., 2000)

Dredd^B118

(Tang et al., 2021, Maitra et al., 2019, Zhai et al., 2018, Liu et al., 2016, Bou Sleiman et al., 2015, Gendrin et al., 2013, Meinander et al., 2012, Maillet et al., 2008, Delaney et al., 2006, Jang et al., 2006, Kim et al., 2006, Ryu et al., 2006)

dredd^B118

(Tarayrah-Ibraheim et al., 2021, Kale et al., 2015, Kuang et al., 2014, Lee et al., 2013, Lee et al., 2013, Wang et al., 2013, Chinchore et al., 2012, Wang et al., 2012, Lee et al., 2011, Berkey et al., 2009, Joza et al., 2008, Baum et al., 2007, Huh et al., 2007, Balakireva et al., 2006, Kim et al., 2006, Kondo et al., 2006, Leulier et al., 2003, Moreno et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (43)

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