Overexpression of timScer\UAS.cYa in PDF neurons under the control of Scer\GAL4P2.4.Pdf does not eliminate circadian rhythms completely, but leads to an increased incidence of arrhythmia. However, no change in fat body triglycerides is observed.
Flies expressing timScer\UAS.cYa under the control of Scer\GAL4tim.PE in a Scer\GAL80Pdf.PS background show rhythmic locomotor activity under constant light conditions, in contrast to wild-type flies, which are uniformly arrhythmic under these conditions.
When timScer\UAS.cYa is driven by Scer\GAL4Mai-60, eclosion rhythms are disrupted. Although peaks are present in the eclosion profile, a rhythm and gating are absent. Adult locomotor activity remains rhythmic as in wild-type. When timScer\UAS.cYa is driven by Scer\GAL4elav-C155 the molecular clock in the lateral neurons is disrupted, and eclosion remains arrhythmic.