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General Information
Symbol
Dmel\timUAS.cYa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Yang
FlyBase ID
FBal0121508
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-tim
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of tim is governed by UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Overexpression of timScer\UAS.cYa in PDF neurons under the control of Scer\GAL4P2.4.Pdf does not eliminate circadian rhythms completely, but leads to an increased incidence of arrhythmia. However, no change in fat body triglycerides is observed.

Flies expressing timScer\UAS.cYa under the control of Scer\GAL4tim.PE in a Scer\GAL80Pdf.PS background show rhythmic locomotor activity under constant light conditions, in contrast to wild-type flies, which are uniformly arrhythmic under these conditions.

When timScer\UAS.cYa is driven by Scer\GAL4Mai-60, eclosion rhythms are disrupted. Although peaks are present in the eclosion profile, a rhythm and gating are absent. Adult locomotor activity remains rhythmic as in wild-type. When timScer\UAS.cYa is driven by Scer\GAL4elav-C155 the molecular clock in the lateral neurons is disrupted, and eclosion remains arrhythmic.

When expression is driven in a tim+ background by Scer\GAL4elav-C155, Scer\GAL4Act5C.PI or Scer\GAL4tim.PE arrythmicity occurs. Two copies of P{UAS-tim.Y} give a stronger effect than one. The effect is not evident with Scer\GAL4P2.4.Pdf.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The severe behavioral arrhythmicity (~70% of flies display arrhythmic circadian activity) observed in flies upon adult-onset expression of mir-276aScer\UAS.T:Ppyr\LUC under the control of Scer\GAL4Pdf.Switch1a (used with RU486-supplemented diet to control the time of expression) is partially rescued by co-expression of timScer\UAS.cYa.

Co-expression of perScer\UAS.cYa and timScer\UAS.cYa under the control of Scer\GAL4P2.4.Pdf results in highly rhythmic flies, with periods close to 24 hours.

The weakened rhythmicity seen in tyfΔG14151 and tyfe00614 flies under constant darkness conditions is not suppressed by expression of timScer\UAS.cYa under the control of Scer\GAL4P2.4.Pdf.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

timScer\UAS.cYa driven by Scer\GAL4elav-C155 does not rescue eclosion rhythms, as it does locomotor rhythms in adults.

The degree of rescue of the tim01 locomotor rhythm defects depends to some extent on the site of insertion of the rescuing P{UAS-tim.Y} transgene and is maximally ~70% and minimally 3.1%. One line, P{UAS-tim.Y}3-1 fails to rescue.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
timScer\UAS.cYa
timUAS.cYa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Yang
Secondary FlyBase IDs
    References (8)