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General Information
Symbol
Dmel\robo3UAS.cSa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Simpson
FlyBase ID
FBal0121545
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-robo3
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of robo3.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of two copies of robo3Scer\UAS.cSa via Scer\GAL412.1 shifts thedbd projection from association with the medial longitudinal fascicle to the intermediate longitudinal fascicle. The dorso-ventral position is unaffected. Occasionally, two branches are formed, one on each of the medial and intermediate fascicles. The dbd projections still undergo proper late stage remodelling, even though their location within the neuropile is abnormal.

Expression of robo3Scer\UAS.cSa in the giant fiber axon, under the regulation of Scer\GAL4A307, deflects the giant fiber axons to the extreme lateral edge of the connective in just under a fifth of the cases and these axons are deflected even further laterally as they approach the target area. Expression of robo3Scer\UAS.cSa, under the control of Scer\GAL4A307 does not affect the latency of the giant fiber, with only subtle defects in following frequency. Ectopic expression of robo3Scer\UAS.cSa, under the control of Scer\GAL4shakB.lethal.4.1, in the tergotrochanteral motor neurons has no effect on the anatomy of the giant fiber-tergotrochanteral motor neuron synapse. Physiologically, ectopic expression of robo3Scer\UAS.cSa under the control of Scer\GAL4shakB.lethal.4.1, in the tergotrochanteral motor neurons has only a subtle effect on giant fiber-tergotrochanteral motor neuron connectivity. Expression of robo3Scer\UAS.cSa under the control of Scer\GAL4c17 results in anatomical defects in the giant fiber (GF) system; a proportion of GFs are deflected laterally. The physiological response of the GFs appears normal in these flies.

Expression of robo3Scer\UAS.cSa under the control of Scer\GAL4GMR.PF has only a minor effect on retinal patterning.

Expression of robo3Scer\UAS.cSa under the control of Scer\GAL4elav.PLu results in a weakly comm-like phenotype. Two copies of robo3Scer\UAS.cSa results in commissureless segments. Some of the Fas2-expressing longitudinal bundles are fused, but at least two distinct fascicles are still seen. Expression of robo3Scer\UAS.cSa under the control of Scer\GAL4ap-md544 leads to an alteration in the lateral position of the ap-expressing neurons, to a position just medial to the intermediate Fas2-expressing pathway.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
Phenotype Manifest In
Enhancer of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of robo3Scer\UAS.cSa enhances the rough eye phenotype caused by expression of AblScer\UAS.cFa under the control of Scer\GAL4GMR.PF.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
robo3Scer\UAS.cSa
robo3UAS.cSa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Simpson
Secondary FlyBase IDs
    References (6)