FlyBase Allele Report: Dmel\robo2[UAS.Tag:HA,Tag:SS(wg)]

General Information

Symbol

Dmel\robo2^{UAS.Tag:HA,Tag:SS(wg)}

Species

D. melanogaster

Name

FlyBase ID

FBal0121552

Feature type

allele

Associated gene

Dmel\robo2

Associated Insertion(s)

Carried in Construct

P{UAS-robo2.HA1}

Also Known As

UAS-robo2, robo2^{Scer\UAS.T:Ivir\HA1,T:wg}

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Rajagopalan et al., 2000, Rajagopalan et al., 2000)

in vitro construct - coding region fusion

(Rajagopalan et al., 2000)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-robo2.HA1}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of robo2 cDNA sequences in which the 5' and 3' untranslated regions have been removed and the 5' sequences encoding the signal sequence have been replaced with sequences encoding the wg signal sequence (Tag:SS(wg)) followed by three copies of the Tag:HA tag.

(Rajagopalan et al., 2000)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav.PLu

(Evans et al., 2015)

abnormal neuroanatomy, with Scer\GAL4^exex-Gal4

(Evans et al., 2015)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^ap-md544

(Evans and Bashaw, 2010)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^elav.PLu

(Evans and Bashaw, 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of robo2^{Scer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^elav.PLu in embryos results in ectopic crossing of the midline by Fas2-positive axons in almost all segments.

Expression of robo2^{Scer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^exex-Gal4 results in Scer\GAL4^exex-Gal4-positive axons shifting to more lateral positions within the neuropile and in Scer\GAL4^exex-Gal4-negative Fas2-expressing axons ectopically crossing the midline.

(Evans et al., 2015)

Ectopic expression of lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg} in embryos under the control of Scer\GAL4^ap-md544 causes axon tracts to select a longitudinal pathway farther from the midline.

Mis-expression of lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^elav.PLu results in thickened commissures and promotes ectopic midline crossing of Fas2-positive axon tracts.

(Evans and Bashaw, 2010)

Single sensory neuron clones expressing lea^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^109(2)80 do not result in disruptions in axon terminal layering or axon morphology.

(Grueber et al., 2007)

12% of ganglionic branches expressing lea^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^bs.Term turn to migrate posteriorly prematurely in embryos.

(Englund et al., 2002)

Expression of one copy of lea^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^ap-md544 shifts ap-expressing neurons (which normally project along the medial edge of the longitudinal tract) laterally. The ap-expressing fascicle often splits into 2 or 3 parallel pathways. Expression of two copies of lea^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^ap-md544 shifts the neurons even further laterally.

(Rajagopalan et al., 2000)

When two copies of P{UAS-robo2.HA1} are driven by Scer\GAL4^elav.PLu commissure formation is prevented. Commissure formation is not prevented when only a single copy of P{UAS-robo2.HA1} is present.

(Rajagopalan et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^ap-md544, robo2^{UAS.Tag:HA,Tag:SS(wg)} has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by robo1¹

(Evans and Bashaw, 2010)

Scer\GAL4^ap-md544, robo2^{UAS.Tag:HA,Tag:SS(wg)} has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by robo3¹

(Evans and Bashaw, 2010)

Scer\GAL4^ap-md544, robo2^{UAS.Tag:HA,Tag:SS(wg)} has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by robo1¹/robo3¹

(Evans and Bashaw, 2010)

Suppressor of

Statement

Reference

robo2^{UAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu is a suppressor of abnormal neuroanatomy phenotype of comm^E39

(Evans et al., 2015)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^ap-md544, robo2^{UAS.Tag:HA,Tag:SS(wg)} has longitudinal connective phenotype, non-suppressible by robo1¹

(Evans and Bashaw, 2010)

Scer\GAL4^ap-md544, robo2^{UAS.Tag:HA,Tag:SS(wg)} has longitudinal connective phenotype, non-suppressible by robo3¹

(Evans and Bashaw, 2010)

Scer\GAL4^ap-md544, robo2^{UAS.Tag:HA,Tag:SS(wg)} has longitudinal connective phenotype, non-suppressible by robo1¹/robo3¹

(Evans and Bashaw, 2010)

Suppressor of

Statement

Reference

robo2^{UAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu is a suppressor of commissure phenotype of comm^E39

(Evans et al., 2015)

NOT Suppressor of

Statement

Reference

robo2^{UAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^bs.Term is a non-suppressor of ganglionic branch primordium phenotype of robo1^unspecified

(Englund et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of robo2^{Scer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^sli.PS suppresses the loss of commissure phenotype of homozygous comm^E39 embryos.

(Evans et al., 2015)

A robo3¹ genetic background does not affect the ability of ectopic Scer\GAL4^ap-md544>lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg} to force the Scer\GAL4^ap-md544-expressing axons to extreme lateral positions.

A robo¹ mutant genetic background does not affect the ability of ectopic Scer\GAL4^ap-md544>lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg} to direct the Scer\GAL4^ap-md544-expressing axons to lateral pathways.

Ectopic Scer\GAL4^ap-md544>lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg} is able to direct the Scer\GAL4^ap-md544-expressing axons to extreme lateral positions even in a robo3¹, robo¹ mutant genetic background.

(Evans and Bashaw, 2010)

The midline crossing phenotype of the ganglionic branches in robo^unspecified mutant embryos is not rescued by expression of lea^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^bs.Term.

(Englund et al., 2002)

Expression of one copy of robo3^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^ap-md544 shifts ap-expressing neurons (which normally project along the medial edge of the longitudinal tract) laterally. Co-expression of lea^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^ap-md544 shifts the neurons even further laterally.

(Rajagopalan et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

robo2^{UAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^bs.Term rescues robo2⁴

(Englund et al., 2002)

Fails to rescue

Scer\GAL4^tracheal/robo2^{UAS.Tag:HA,Tag:SS(wg)} fails to rescue robo2^x135

(Parsons et al., 2003)

Scer\GAL4^elav-C155/robo2^{UAS.Tag:HA,Tag:SS(wg)} fails to rescue robo2^x135

(Parsons et al., 2003)

Comments

The frequency of dorsal and lateral cluster sensory axon defects seen in lea^x135 embryos is not rescued by expression of lea^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^elav-C155 or Scer\GAL4^tracheal.

(Parsons et al., 2003)

Expression of lea^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^bs.Term rescues the stalling of ganglionic branches outside the central nervous system that is seen in lea^robo2-4 embryos. There is no reduction in the number of ganglionic branches that cross the midline in these embryos and 17% of GB1 cells turn prematurely before reaching the midline.

(Englund et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

66886

y¹ w^*; P{UAS-robo2.HA1}1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg}

lea^{Scer\UAS.T:Ivir\HA1,T:wg}

robo2^{Scer\UAS.T:Ivir\HA1,T:SS-wg}

robo2^{Scer\UAS.T:Ivir\HA1,T:wg}

(Rajagopalan et al., 2000, Rajagopalan et al., 2000)

robo2^{UAS.Tag:HA,Tag:SS(wg)}

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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