Allele Dmel\lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg}

General Information
Symbol	Dmel\lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg}	Species	D. melanogaster
Name		FlyBase ID	FBal0121552
Feature type	allele	Associated gene	Dmel\lea
Also Known As	robo2^{Scer\UAS.T:Ivir\HA1,T:wg}

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Rajagopalan et al., 2000) in vitro construct - regulatory fusion (Rajagopalan et al., 2000, Rajagopalan et al., 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of lea cDNA sequences in which the 5' and 3' untranslated regions have been removed and the 5' sequences encoding the signal sequence have been replaced with sequences encoding the wg signal sequence (T:SS-wg) followed by three copies of the T:Ivir\HA1 tag. (Rajagopalan et al., 2000)
Carried in construct	P{UAS-robo2.HA1} (Qian et al., 2005, Englund et al., 2002, Rajagopalan et al., 2000, Rajagopalan et al., 2000, Parsons et al., 2003, Grueber et al., 2007)
Tagged with	T:Ivir\HA1 (Rajagopalan et al., 2000, Rajagopalan et al., 2000, Englund et al., 2002, Parsons et al., 2003, Qian et al., 2005) T:SS-wg (Rajagopalan et al., 2000, Rajagopalan et al., 2000, Englund et al., 2002, Parsons et al., 2003, Qian et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	embryonic/larval neuron, with Scer\GAL4^ap-md544 (Rajagopalan et al., 2000) ganglionic branch primordium, with Scer\GAL4^bs.Term (Englund et al., 2002) ventral nerve cord commissure, with Scer\GAL4^elav.PLu (Rajagopalan et al., 2000)
Detailed Description
	Statement Reference Single sensory neuron clones expressing lea[Scer\UAS.T:Ivir\HA1,T:wg] under the control of Scer\GAL4[109(2)80] do not result in disruptions in axon terminal layering or axon morphology. (Grueber et al., 2007) 12% of ganglionic branches expressing lea^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^bs.Term turn to migrate posteriorly prematurely in embryos. (Englund et al., 2002) Expression of one copy of lea^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^ap-md544 shifts ap-expressing neurons (which normally project along the medial edge of the longitudinal tract) laterally. The ap-expressing fascicle often splits into 2 or 3 parallel pathways. Expression of two copies of lea^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^ap-md544 shifts the neurons even further laterally. (Rajagopalan et al., 2000) When two copies of P{UAS-robo2.HA1} are driven by Scer\GAL4^elav.PLu commissure formation is prevented. Commissure formation is not prevented when only a single copy of P{UAS-robo2.HA1} is present. (Rajagopalan et al., 2000)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
NOT Suppressor of
Statement Reference lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg}/Scer\GAL4^bs.Term is a non-suppressor of ganglionic branch primordium phenotype of robo^unspecified (Englund et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference The midline crossing phenotype of the ganglionic branches in robo^unspecified mutant embryos is not rescued by expression of lea^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^bs.Term. (Englund et al., 2002) Expression of one copy of robo3^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^ap-md544 shifts ap-expressing neurons (which normally project along the medial edge of the longitudinal tract) laterally. Co-expression of lea^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^ap-md544 shifts the neurons even further laterally. (Rajagopalan et al., 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg}/Scer\GAL4^bs.Term rescues lea^robo2-4 (Englund et al., 2002)
Fails to rescue	Scer\GAL4^elav-C155/lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg} fails to rescue lea^x135 (Parsons et al., 2003) Scer\GAL4^tracheal/lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg} fails to rescue lea^x135 (Parsons et al., 2003)
Comments	The frequency of dorsal and lateral cluster sensory axon defects seen in lea^x135 embryos is not rescued by expression of lea^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^elav-C155 or Scer\GAL4^tracheal. (Parsons et al., 2003) Expression of lea^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^bs.Term rescues the stalling of ganglionic branches outside the central nervous system that is seen in lea^robo2-4 embryos. There is no reduction in the number of ganglionic branches that cross the midline in these embryos and 17% of GB1 cells turn prematurely before reaching the midline. (Englund et al., 2002)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg} lea^{Scer\UAS.T:Ivir\HA1,T:wg} robo2^{Scer\UAS.T:Ivir\HA1,T:wg} (Rajagopalan et al., 2000, Rajagopalan et al., 2000, )
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Grueber et al., 2007, Development 134(1): 55--64 Projections of Drosophila multidendritic neurons in the central nervous system: links with peripheral dendrite morphology. [FBrf0194377] Qian et al., 2005, Curr. Biol. 15(24): 2271--2278 Slit and Robo control cardiac cell polarity and morphogenesis. [FBrf0190040] Parsons et al., 2003, Dev. Biol. 264(2): 363--375 roundabout gene family functions during sensory axon guidance in the Drosophila embryo are mediated by both Slit-dependent and Slit-independent mechanisms. [FBrf0167462] Englund et al., 2002, Development 129(21): 4941--4951 Attractive and repulsive functions of Slit are mediated by different receptors in the Drosophila trachea. [FBrf0151931] Rajagopalan et al., 2000, Neuron 28(3): 767--777 Crossing the midline: roles and regulation of Robo receptors. [FBrf0132432] Rajagopalan et al., 2000, Cell 103(7): 1033--1045 Selecting a longitudinal pathway: robo receptors specify the lateral position of axons in the Drosophila CNS. [FBrf0132258]

Allele Dmel\leaScer\UAS.T:Ivir\HA1,T:SS-wg

Allele Dmel\lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg}