UASt regulatory sequences drive expression of robo2 cDNA sequences in which the 5' and 3' untranslated regions have been removed and the 5' sequences encoding the signal sequence have been replaced with sequences encoding the wg signal sequence (Tag:SS(wg)) followed by three copies of the Tag:HA tag.
Expression of one copy of robo3Scer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4ap-md544 shifts ap-expressing neurons (which normally project along the medial edge of the longitudinal tract) laterally. Co-expression of leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4ap-md544 shifts the neurons even further laterally.
The frequency of dorsal and lateral cluster sensory axon defects seen in leax135 embryos is not rescued by expression of leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4elav-C155 or Scer\GAL4tracheal.
Expression of leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4bs.Term rescues the stalling of ganglionic branches outside the central nervous system that is seen in learobo2-4 embryos. There is no reduction in the number of ganglionic branches that cross the midline in these embryos and 17% of GB1 cells turn prematurely before reaching the midline.