|Feature type||allele||Associated gene||Dmel\lea|
|Also Known As||robo2Scer\UAS.T:Ivir\HA1,T:wg|
|Mutagen||in vitro construct - regulatory fusion, in vitro construct - coding region fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Scer\UAS regulatory sequences drive expression of lea cDNA sequences in which the 5' and 3' untranslated regions have been removed and the 5' sequences encoding the signal sequence have been replaced with sequences encoding the wg signal sequence (T:SS-wg) followed by three copies of the T:Ivir\HA1 tag.
|Carried in construct|
|Phenotype Manifest In|
Single sensory neuron clones expressing lea[Scer\UAS.T:Ivir\HA1,T:wg] under the control of Scer\GAL4[109(2)80] do not result in disruptions in axon terminal layering or axon morphology.
12% of ganglionic branches expressing leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4bs.Term turn to migrate posteriorly prematurely in embryos.
Expression of one copy of leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4ap-md544 shifts ap-expressing neurons (which normally project along the medial edge of the longitudinal tract) laterally. The ap-expressing fascicle often splits into 2 or 3 parallel pathways. Expression of two copies of leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4ap-md544 shifts the neurons even further laterally.
|Phenotype Manifest In|
|NOT Suppressor of|
The midline crossing phenotype of the ganglionic branches in robounspecified mutant embryos is not rescued by expression of leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4bs.Term.
Expression of one copy of robo3Scer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4ap-md544 shifts ap-expressing neurons (which normally project along the medial edge of the longitudinal tract) laterally. Co-expression of leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4ap-md544 shifts the neurons even further laterally.
|Complementation & Rescue Data|
|Fails to rescue|
The frequency of dorsal and lateral cluster sensory axon defects seen in leax135 embryos is not rescued by expression of leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4elav-C155 or Scer\GAL4tracheal.
Expression of leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4bs.Term rescues the stalling of ganglionic branches outside the central nervous system that is seen in learobo2-4 embryos. There is no reduction in the number of ganglionic branches that cross the midline in these embryos and 17% of GB1 cells turn prematurely before reaching the midline.
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
|Secondary FlyBase IDs|
|References ( 6 )|