Open Close
General Information
Symbol
Dmel\robo2UAS.Tag:HA,Tag:SS(wg)
Species
D. melanogaster
Name
FlyBase ID
FBal0121552
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-robo2, robo2Scer\UAS.T:Ivir\HA1,T:wg
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of robo2 cDNA sequences in which the 5' and 3' untranslated regions have been removed and the 5' sequences encoding the signal sequence have been replaced with sequences encoding the wg signal sequence (Tag:SS(wg)) followed by three copies of the Tag:HA tag.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of robo2Scer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4elav.PLu in embryos results in ectopic crossing of the midline by Fas2-positive axons in almost all segments.

Expression of robo2Scer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4exex-Gal4 results in Scer\GAL4exex-Gal4-positive axons shifting to more lateral positions within the neuropile and in Scer\GAL4exex-Gal4-negative Fas2-expressing axons ectopically crossing the midline.

Ectopic expression of leaScer\UAS.T:Ivir\HA1,T:SS-wg in embryos under the control of Scer\GAL4ap-md544 causes axon tracts to select a longitudinal pathway farther from the midline.

Mis-expression of leaScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4elav.PLu results in thickened commissures and promotes ectopic midline crossing of Fas2-positive axon tracts.

Single sensory neuron clones expressing leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4109(2)80 do not result in disruptions in axon terminal layering or axon morphology.

12% of ganglionic branches expressing leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4bs.Term turn to migrate posteriorly prematurely in embryos.

Expression of one copy of leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4ap-md544 shifts ap-expressing neurons (which normally project along the medial edge of the longitudinal tract) laterally. The ap-expressing fascicle often splits into 2 or 3 parallel pathways. Expression of two copies of leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4ap-md544 shifts the neurons even further laterally.

When two copies of P{UAS-robo2.HA1} are driven by Scer\GAL4elav.PLu commissure formation is prevented. Commissure formation is not prevented when only a single copy of P{UAS-robo2.HA1} is present.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of robo2Scer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4sli.PS suppresses the loss of commissure phenotype of homozygous commE39 embryos.

A robo31 genetic background does not affect the ability of ectopic Scer\GAL4ap-md544>leaScer\UAS.T:Ivir\HA1,T:SS-wg to force the Scer\GAL4ap-md544-expressing axons to extreme lateral positions.

A robo1 mutant genetic background does not affect the ability of ectopic Scer\GAL4ap-md544>leaScer\UAS.T:Ivir\HA1,T:SS-wg to direct the Scer\GAL4ap-md544-expressing axons to lateral pathways.

Ectopic Scer\GAL4ap-md544>leaScer\UAS.T:Ivir\HA1,T:SS-wg is able to direct the Scer\GAL4ap-md544-expressing axons to extreme lateral positions even in a robo31, robo1 mutant genetic background.

The midline crossing phenotype of the ganglionic branches in robounspecified mutant embryos is not rescued by expression of leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4bs.Term.

Expression of one copy of robo3Scer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4ap-md544 shifts ap-expressing neurons (which normally project along the medial edge of the longitudinal tract) laterally. Co-expression of leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4ap-md544 shifts the neurons even further laterally.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The frequency of dorsal and lateral cluster sensory axon defects seen in leax135 embryos is not rescued by expression of leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4elav-C155 or Scer\GAL4tracheal.

Expression of leaScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4bs.Term rescues the stalling of ganglionic branches outside the central nervous system that is seen in learobo2-4 embryos. There is no reduction in the number of ganglionic branches that cross the midline in these embryos and 17% of GB1 cells turn prematurely before reaching the midline.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
leaScer\UAS.T:Ivir\HA1,T:SS-wg
leaScer\UAS.T:Ivir\HA1,T:wg
robo2Scer\UAS.T:Ivir\HA1,T:SS-wg
robo2Scer\UAS.T:Ivir\HA1,T:wg
robo2UAS.Tag:HA,Tag:SS(wg)
Name Synonyms
Secondary FlyBase IDs
    References (11)