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General Information
Symbol
Dmel\robo2EP2582
Species
D. melanogaster
Name
FlyBase ID
FBal0121553
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
EP2582, EP(2)2582
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The P{EP} element is inserted 101 nucleotides 5' to the predicted mRNA start.

P{EP} insertion upstream of lea.

Insertion components
P{EP}robo2EP2582
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In leaEP2582; Scer\GAL4eg-Mz360 embryos, serotonergic axons do not cross the midline. Despite their defects in serotonergic neuronal axon guidance, these neurons retain their normal ability to take up serotonin. Unlike wild-type serotonergic neurons, the ability of serotonergic neurons in the ventral nerve cord of stage 16 leaEP2582; Scer\GAL4eg-Mz360 embryos to take up serotonin is not lost when their axonal contacts with the midline are severed. The cell bodies of serotonergic neurons in the ventral nerve cord of stage 16 leaEP2582; Scer\GAL4eg-Mz360 embryos are shifted laterally compared to wild-type.

Antennal commissure formation is abolished and glomerular morphology is disrupted in the antennal lobes of leaEP2582; Scer\GAL4SG18.1 flies.

Abdominal lch5 chordotonal organs are transformed so that they resemble thoracic dch3 chordotonal organs in larvae expressing leaEP2582 under the control of Scer\GAL4elav-C155. The transformed "lch5" chordotonal organs are displaced dorsally in the ectoderm. They have the usual number of five neurons per cluster, but their dendrites and support cells show a variety of orientations, which range from the ventral orientation typical of dch3 organs, through intermediate bent orientations, to orientations similar to those of normal lch5 organs. At 29oC, 44% of abdominal segments have partial or complete transformation of lch5 chordotonal organ morphology. At 25oC, 32% of abdominal segments have partial or complete transformation of lch5 chordotonal organ morphology. Defects indicating transformation of the lch5 chordotonal organs can be detected as early as stage 14 in embryos expressing leaEP2582 under the control of Scer\GAL4elav-C155. The cap cells of the transformed "lch5" organs always follow the orientation of the transformed neurons. At 29oC, 30.5% of abdominal segments have partial or complete transformation of lch5 chordotonal organ morphology towards that of the thoracic dch3 chordotonal organs, in larvae expressing leaEP2582 under the control of Scer\GAL4ato.3.6. Some abdominal lch5 chordotonal organs fail to migrate ventrally (as occurs in wild type) in embryos expressing leaEP2582 under the control of Scer\GAL4ato.3.6. Some of these "stalled" abdominal clusters extend projections dorsally. At 29oC, 17% of abdominal segments have partial or complete transformation of lch5 chordotonal organ morphology towards that of the thoracic dch3 chordotonal organs, in embryos expressing leaEP2582 under the control of Scer\GAL448Y.

ventral nerve cord commissures in stage 17 leaEP2582; Scer\GAL4sca-4512 embryos are missing or fused.

Mutant larvae expressing leaEP2582 under the control of Scer\GAL4elav-C155 have ISN neuron pathfinding defects.

When expression of leaEP2582 is driven by Scer\GAL4elav.PLu, commissures are lost.

Expression of leaEP2582 under the control of Scer\GAL4ap-md544 causes ap-expressing neurons to move laterally (relative to wild type) and extend anteriorly in a specific location between the intermediate and lateral Fas2-expressing pathways. The ap-expressing axons from neighbouring segments appear to pick the same lateral pathway and to fasciculate together as they extend anteriorly from segment to segment. Expression of leaEP2582 under the control of Scer\GAL415J2 results in a bimodal phenotype. The dMP2 and vMP2 axons always appear to extend in a Fas2-expressing pathway, but they are now found in either the intermediate or lateral pathways (they normally extend in the medial pathway). They are usually found in the intermediate pathway and occasionally found in the lateral pathway.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
NOT Enhancer of
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Suppressed by
NOT suppressed by
NOT Enhancer of
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The expression of robo2EP2582 under the control of Scer\GAL4esg.PU fully or partially suppresses the increased proportion of pre-enteroendocrine cells and the reciprocal decreased proportion of mature enteroendocrine cells, respectively, observed in the adult gut of Atg16d67 or Atg16MI00187 homozygotes; this expression also suppresses the severely decreased lifespan of Atg16d67 or Atg16MI00187 homozygotes upon oral infection with Pseudomonas aeruginosa or Enterococcus faecalis.

Commissure formation and glomerular morphology in the antennal lobes of leaEP2582; Scer\GAL4SG18.1 flies are restored by Df(2R)WMG/+.

The transformation of abdominal lch5 chordotonal organs to a morphology resembling thoracic dch3 chordotonal organs that is seen in larvae expressing leaEP2582 under the control of Scer\GAL4elav-C155 is still seen if they are also mutant for robo7; 36.4% of abdominal segments have partial or complete transformation of the lch5 organs in the double mutant larvae. The transformation of abdominal lch5 chordotonal organs to a morphology resembling thoracic dch3 chordotonal organs that is seen in larvae expressing leaEP2582 under the control of Scer\GAL4elav-C155 is attenuated if the larvae also carry one copy of sli2; only 17.2% of abdominal segments have partial or complete transformation of the lch5 organs in the double mutant larvae at 25oC.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (16)