Allele Dmel\lea^robo2-5

General Information
Symbol	Dmel\lea^robo2-5	Species	D. melanogaster
Name		FlyBase ID	FBal0121558
Feature type	allele	Associated gene	Dmel\lea
Also Known As	robo2⁵

Map ( GBrowse )
Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Rajagopalan et al., 2000)
Mutagen	ethyl methanesulfonate (Rajagopalan et al., 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2L:1,391,392..1,391,393 reported_pr_change=W723@ comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified) evidence=experimental pr_change=W723@\|lea-PA (Rajagopalan et al., 2000)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	lea^EP2582 (Rajagopalan et al., 2000)
Nature of the lesion	Statement Reference Amino acid replacement: W666@ (revision of data in FBrf0132258). Amino acid replacements are relative to the 1406 amino acid residue predicted protein. (Dickson, 2001.3.14) Amino acid replacement: W723@. See Dickson, 2001.3.14, personal communication for subsequent revision of this information. (Rajagopalan et al., 2000)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective (with lea^robo2-1) (Nicolas and Preat, 2005) neuroanatomy defective (with lea^robo2-4) (Nicolas and Preat, 2005)
Phenotype Manifest In
	adult mushroom body (with lea^robo2-1) (Nicolas and Preat, 2005) adult mushroom body (with lea^robo2-4) (Nicolas and Preat, 2005) ellipsoid body (with lea^robo2-1) (Nicolas and Preat, 2005) ellipsoid body (with lea^robo2-4) (Nicolas and Preat, 2005) fan-shaped body (with lea^robo2-1) (Nicolas and Preat, 2005) fan-shaped body (with lea^robo2-4) (Nicolas and Preat, 2005) mushroom body medial lobe (with lea^robo2-1) (Nicolas and Preat, 2005) mushroom body medial lobe (with lea^robo2-4) (Nicolas and Preat, 2005) mushroom body vertical lobe (with lea^robo2-1) (Nicolas and Preat, 2005) mushroom body vertical lobe (with lea^robo2-4) (Nicolas and Preat, 2005)
Detailed Description
	Statement Reference lea^robo2-4/lea^robo2-5 and lea^robo2-1/lea^robo2-5 have defects in the ellipsoid body and fan-shaped body. 16% of mushroom bodies lack dorsal projections in lea^robo2-1/lea^robo2-5 animals, and 32% have a partial median projection. In addition, the diameter of the peduncle is larger than in wild-type animals. 12% of mushroom bodies lack dorsal projections in lea^robo2-4/lea^robo2-5 animals, and 8% have a partial median projection. In addition, the the diameter of peduncle is larger than in wild-type animals. (Nicolas and Preat, 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference lea^robo2-5, robo^unspecified has neuroanatomy defective phenotype, enhanceable by Abl²/Abl[+] (Wills et al., 2002)
Other
Statement Reference lea^robo2-5, robo^unspecified has neuroanatomy defective phenotype (Wills et al., 2002)
Phenotype Manifest In
Enhanced by
Statement Reference lea^robo2-5, robo^unspecified has presumptive embryonic/larval central nervous system phenotype, enhanceable by Abl²/Abl[+] (Wills et al., 2002) lea^robo2-5 has aCC neuron phenotype, enhanceable by robo¹ (Rajagopalan et al., 2000) lea^robo2-5 has embryonic/larval muscle system phenotype, enhanceable by robo¹ (Rajagopalan et al., 2000) lea^robo2-5 has pCC neuron phenotype, enhanceable by robo¹ (Rajagopalan et al., 2000)
NOT suppressed by
Statement Reference lea^robo2-5, robo¹ has ventral nerve cord phenotype, non-suppressible by comm¹ (Rajagopalan et al., 2000)
Enhancer of
Statement Reference lea^robo2-5 is an enhancer of aCC neuron phenotype of robo¹ (Rajagopalan et al., 2000) lea^robo2-5 is an enhancer of embryonic/larval muscle system phenotype of robo¹ (Rajagopalan et al., 2000) lea^robo2-5 is an enhancer of pCC neuron phenotype of robo¹ (Rajagopalan et al., 2000)
NOT Enhancer of
Statement Reference lea^robo2-5 is a non-enhancer of ventral nerve cord commissure phenotype of comm¹ (Rajagopalan et al., 2000)
NOT Suppressor of
Statement Reference lea^robo2-5 is a non-suppressor of ventral nerve cord commissure phenotype of comm¹ (Rajagopalan et al., 2000)
Other
Statement Reference lea^robo2-5, robo^unspecified has presumptive embryonic/larval central nervous system phenotype (Wills et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference The frequency of ectopic crossing of the midline by axons in the central nervous system seen in robo^unspecified lea^robo2-5 embryos is increased by Abl²/+. (Wills et al., 2002) robo¹/lea^robo2-5 embryos show an enhancement of the embryonic nervous system phenotype seen in these embryos. The pCC axon fasciculates with its contralateral homologue to extend anteriorly right along the midline. The aCC axons also frequently cross the midline in these embryos. By stage 16, all axons have joined the single midline bundle. Double mutants also exhibit a embryonic muscle phenotype. Ventral muscles normally attach to the epidermis beneath the lateral central nervous system (CNS). In robo¹/lea^robo2-5 mutants, these muscles frequently extend across the dorsal surface of the CNS. Some muscles still attach to beneath the CNS though often somewhat close to the midline than usual. The addition of lea^robo2-5 to comm¹ embryos has no effect on the number of commissures crossing the midline in the ventral nerve cords in these mutants. The addition of comm¹ to robo¹, lea^robo2-5 embryos has no effect on the ventral nerve cord phenotype. (Rajagopalan et al., 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	lea^robo2-5 robo2⁵ (Wills et al., 2002, Englund et al., 2002, Dickson, 2001.3.14, Rajagopalan et al., 2000, Rajagopalan et al., 2000, Nicolas and Preat, 2005)
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Nicolas and Preat, 2005, Dev. Genes Evol. 215(10): 530--536 Drosophila central brain formation requires Robo proteins. [FBrf0190257] Englund et al., 2002, Development 129(21): 4941--4951 Attractive and repulsive functions of Slit are mediated by different receptors in the Drosophila trachea. [FBrf0151931] Wills et al., 2002, Neuron 36(4): 611--622 A Drosophila homolog of cyclase-associated proteins collaborates with the abl tyrosine kinase to control midline axon pathfinding. [FBrf0152273] Rajagopalan et al., 2000, Neuron 28(3): 767--777 Crossing the midline: roles and regulation of Robo receptors. [FBrf0132432] Rajagopalan et al., 2000, Cell 103(7): 1033--1045 Selecting a longitudinal pathway: robo receptors specify the lateral position of axons in the Drosophila CNS. [FBrf0132258]
Personal communication to FlyBase	Dickson, 2001.3.14, Molecular details of robo2 alleles. Molecular details of robo2 alleles. [FBrf0135264]

Allele Dmel\learobo2-5

Allele Dmel\lea^robo2-5