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General Information
Symbol
Dmel\robo24
Species
D. melanogaster
Name
FlyBase ID
FBal0121559
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G1391735A

Amino acid change:

W635term | robo2-PA; W635term | robo2-PB

Reported amino acid change:

W635term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacements are a revision of the data reported in FBrf0132258. They are numbered relative to the 1406 amino acid residue predicted protein.

Amino acid replacement: W578term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The adult sLNv neurons of robo24/robo29 transheterozygotes show several morphological defects of variable penetrance, including axon projection defects and mis-placed cell body, as compared to controls; however, the few mutant axons that do reach the calyx display normal length of the terminal branches.

learobo2-8/learobo2-4 stage 13 embryos have gonads with unfused somatic gonadal precursor (SGP) clusters. By stage 15, SGP clusters fuse but gonads then fail to compact properly. Mutants also show germ cell ensheathment defects.

Fas2-positive longitudinal axons show a number of defects in learobo2-4/learobo2-8 stage 16 embryos; Fas2-positive axons extend across or along the midline in 23.7% of segments, intermediate Fas2-positive fascicles are fused or broken in 10.2% of hemisegments and lateral Fas2-positive fascicles are fused or broken in 29.9% of hemisegments.

learobo2-4/learobo2-5 and learobo2-1/learobo2-4 have defects in the ellipsoid body and fan-shaped body.

12% of mushroom bodies lack dorsal projections in learobo2-4/learobo2-5 animals, and 8% have a partial median projection. In addition, the the diameter of peduncle is larger than in wild-type animals.

34% of mushroom bodies lack dorsal projections in learobo2-4/learobo2-1 animals, and 18% have a partial median projection. The median lobes are positioned more posteriorly than in wild type. In addition, the the diameter of peduncle is larger than in wild-type animals.

6% of ganglionic branches cross the midline in mutant embryos. These ganglionic branches do not extend to the contralateral longitudinal connectives but linger around the midline instead. Ganglionic branches often stall outside the central nervous system in these embryos. 6% of ganglionic branches cross the midline in learobo2-4/learobo2-8 embryos and 15% of ganglionic branches fail to enter the central nervous system.

The lateral Fas2-expressing fascicle often merges with the intermediate fascicle in learobo2-1/learobo2-4 mutant embryos. Midline crossing defects also occur. 7.9% of embryos show fusion between the medial and intermediate fascicles, 17.1% show fusion between the lateral and and intermediate fascicles and 13.4% show breaks in the lateral fascicle.

In learobo2-1/learobo2-4 mutants about a quarter of Fas2 positive axons in the ventral nerve cord are misrouted. These form bundles of varying thickness.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
NOT Enhancer of
Statement
Reference
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Stage 16-17 Df(1)NetABΔ ; learobo2-4 double mutant embryos almost completely lack commissures; 70.7% of anterior commissures and 87.1% of posterior commissures are absent, while 27.6% of anterior commissures and 12.9% of posterior commissures are thin.

Expression of roboScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4bs.Term partially rescues the failure of ganglionic branches to enter the central nervous system that is seen in learobo2-4/learobo2-8 embryos and the weak midline crossing phenotype of the ganglionic branches seen in these embryos is also rescued.

capt10 learobo2-4 double heterozygotes show little if any midline crossing defects in the embryonic central nervous system. The frequency of ectopic crossing of the midline by axons in the central nervous system seen in robounspecified learobo2-4 embryos is increased by Abl2/+ or Abl4/+.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (14)