W635term | robo2-PA; W635term | robo2-PB
G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.
Stage 16-17 Df(1)NetABΔ ; learobo2-4 double mutant embryos almost completely lack commissures; 70.7% of anterior commissures and 87.1% of posterior commissures are absent, while 27.6% of anterior commissures and 12.9% of posterior commissures are thin.
Expression of roboScer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4bs.Term partially rescues the failure of ganglionic branches to enter the central nervous system that is seen in learobo2-4/learobo2-8 embryos and the weak midline crossing phenotype of the ganglionic branches seen in these embryos is also rescued.
capt10 learobo2-4 double heterozygotes show little if any midline crossing defects in the embryonic central nervous system. The frequency of ectopic crossing of the midline by axons in the central nervous system seen in robounspecified learobo2-4 embryos is increased by Abl2/+ or Abl4/+.