Open Close
General Information
Symbol
Dmel\robo21
Species
D. melanogaster
Name
FlyBase ID
FBal0121562
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C1392318T

Amino acid change:

R441term | robo2-PA; R441term | robo2-PB

Reported amino acid change:

R441term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacements are a revision of the data reported in FBrf0132258. They are numbered relative to the 1406 amino acid residue predicted protein.

Amino acid replacement: R384term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

learobo2-1 embryos show weak salivary gland guidance defects.

learobo2-1/learobo2-5 and learobo2-1/learobo2-4 have defects in the ellipsoid body and fan-shaped body.

16% of mushroom bodies lack dorsal projections in learobo2-1/learobo2-5 animals, and 32% have a partial median projection. In addition, the diameter of the peduncle is larger than in wild-type animals.

34% of mushroom bodies lack dorsal projections in learobo2-4/learobo2-1 animals, and 18% have a partial median projection. The median lobes are positioned more posteriorly than in wild type. In addition, the the diameter of peduncle is larger than in wild-type animals.

learobo2-1/learobo2-1 DM2 glomerulus innervating neurons, produced by mitotic recombination in a learobo2-1/+ background, fail to cross over to the contralateral lobe through the antennal commissure. Instead they terminate at the midline forming small "glomerular-like" structures. Targeting of these neurons to antennal glomerulus DM2 occurs normally, although in many cases the glomeruli appear less intensely innervated (13/16).

The lateral Fas2-expressing fascicle often merges with the intermediate fascicle in learobo2-1/learobo2-4 mutant embryos. Midline crossing defects also occur. 7.9% of embryos show fusion between the medial and intermediate fascicles, 17.1% show fusion between the lateral and and intermediate fascicles and 13.4% show breaks in the lateral fascicle.

In learobo2-1/learobo2-4 mutants about a quarter of Fas2 positive axons in the ventral nerve cord are misrouted. These form bundles of varying thickness.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference
Enhancer of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

In robo1 learobo2-1 double mutants, the salivary gland guidance phenotype is enhanced. Glands are curved medially towards the midline, instead of lying parallel to the midline as in wild type.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (9)