Allele Dmel\lea^robo2-1

General Information
Symbol	Dmel\lea^robo2-1	Species	D. melanogaster
Name		FlyBase ID	FBal0121562
Feature type	allele	Associated gene	Dmel\lea
Also Known As	robo2¹

Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Rajagopalan et al., 2000, Sano et al., 2005)
Mutagen	ethyl methanesulfonate (Rajagopalan et al., 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	lea^EP2582 (Rajagopalan et al., 2000)
Nature of the lesion	Statement Reference Amino acid replacement: R384@ (revision of data in FBrf0132258). Amino acid replacements are relative to the 1406 amino acid residue predicted protein. (Dickson, 2001.3.14) Amino acid replacement: R441@. See Dickson, 2001.3.14, personal communication for subsequent revision of this information. (Rajagopalan et al., 2000)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective (with lea^robo2-4) (Nicolas and Preat, 2005) neuroanatomy defective (with lea^robo2-5) (Nicolas and Preat, 2005)
Phenotype Manifest In
	adult mushroom body (with lea^robo2-4) (Nicolas and Preat, 2005) adult mushroom body (with lea^robo2-5) (Nicolas and Preat, 2005) antennal commissure \| somatic clone (Jhaveri et al., 2004) antennal lobe glomerulus \| ectopic \| somatic clone (Jhaveri et al., 2004) antennal lobe glomerulus DM2 \| somatic clone (Jhaveri et al., 2004) ellipsoid body (with lea^robo2-4) (Nicolas and Preat, 2005) ellipsoid body (with lea^robo2-5) (Nicolas and Preat, 2005) fan-shaped body (with lea^robo2-4) (Nicolas and Preat, 2005) fan-shaped body (with lea^robo2-5) (Nicolas and Preat, 2005) longitudinal connective (with lea^robo2-4) (Rajagopalan et al., 2000, Rajagopalan et al., 2000) mushroom body medial lobe (with lea^robo2-4) (Nicolas and Preat, 2005) mushroom body medial lobe (with lea^robo2-5) (Nicolas and Preat, 2005) mushroom body vertical lobe (with lea^robo2-4) (Nicolas and Preat, 2005) mushroom body vertical lobe (with lea^robo2-5) (Nicolas and Preat, 2005) olfactory neuron & antennal lobe \| somatic clone (Jhaveri et al., 2004) presumptive embryonic salivary gland (Kolesnikov and Beckendorf, 2005)
Detailed Description
	Statement Reference lea^robo2-1 embryos show weak salivary gland guidance defects. (Kolesnikov and Beckendorf, 2005) lea^robo2-1/lea^robo2-5 and lea^robo2-1/lea^robo2-4 have defects in the ellipsoid body and fan-shaped body. 16% of mushroom bodies lack dorsal projections in lea^robo2-1/lea^robo2-5 animals, and 32% have a partial median projection. In addition, the diameter of the peduncle is larger than in wild-type animals. 34% of mushroom bodies lack dorsal projections in lea^robo2-4/lea^robo2-1 animals, and 18% have a partial median projection. The median lobes are positioned more posteriorly than in wild type. In addition, the the diameter of peduncle is larger than in wild-type animals. (Nicolas and Preat, 2005) lea^robo2-1/lea^robo2-1 DM2 glomerulus innervating neurons, produced by mitotic recombination in a lea^robo2-1/+ background, fail to cross over to the contralateral lobe through the antennal commissure. Instead they terminate at the midline forming small "glomerular-like" structures. Targeting of these neurons to antennal glomerulus DM2 occurs normally, although in many cases the glomeruli appear less intensely innervated (13/16). (Jhaveri et al., 2004) The lateral Fas2-expressing fascicle often merges with the intermediate fascicle in lea^robo2-1/lea^robo2-4 mutant embryos. Midline crossing defects also occur. 7.9% of embryos show fusion between the medial and intermediate fascicles, 17.1% show fusion between the lateral and and intermediate fascicles and 13.4% show breaks in the lateral fascicle. (Rajagopalan et al., 2000) In lea^robo2-1/lea^robo2-4 mutants about a quarter of Fas2 positive axons in the ventral nerve cord are misrouted. These form bundles of varying thickness. (Rajagopalan et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference lea^robo2-1 has presumptive embryonic salivary gland phenotype, enhanceable by robo¹ (Kolesnikov and Beckendorf, 2005)
Enhancer of
Statement Reference lea^robo2-1 is an enhancer of presumptive embryonic salivary gland phenotype of robo¹ (Kolesnikov and Beckendorf, 2005)
Additional Comments
Genetic Interactions
Statement Reference In robo¹ lea^robo2-1 double mutants, the salivary gland guidance phenotype is enhanced. Glands are curved medially towards the midline, instead of lying parallel to the midline as in wild type. (Kolesnikov and Beckendorf, 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	lea^robo2-1 robo2¹ (Kolesnikov and Beckendorf, 2005, Jhaveri et al., 2004, Englund et al., 2002, Dickson, 2001.3.14, Rajagopalan et al., 2000, Rajagopalan et al., 2000, Nicolas and Preat, 2005, Sano et al., 2005)
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Kolesnikov and Beckendorf, 2005, Dev. Biol. 284(1): 102--111 NETRIN and SLIT guide salivary gland migration. [FBrf0187388] Nicolas and Preat, 2005, Dev. Genes Evol. 215(10): 530--536 Drosophila central brain formation requires Robo proteins. [FBrf0190257] Sano et al., 2005, J. Cell Biol. 171(4): 675--683 Control of lateral migration and germ cell elimination by the Drosophila melanogaster lipid phosphate phosphatases Wunen and Wunen 2. [FBrf0190943] Jhaveri et al., 2004, Development 131(9): 1903--1912 Positioning sensory terminals in the olfactory lobe of Drosophila by Robo signaling. [FBrf0174568] Englund et al., 2002, Development 129(21): 4941--4951 Attractive and repulsive functions of Slit are mediated by different receptors in the Drosophila trachea. [FBrf0151931] Rajagopalan et al., 2000, Neuron 28(3): 767--777 Crossing the midline: roles and regulation of Robo receptors. [FBrf0132432] Rajagopalan et al., 2000, Cell 103(7): 1033--1045 Selecting a longitudinal pathway: robo receptors specify the lateral position of axons in the Drosophila CNS. [FBrf0132258]
Personal communication to FlyBase	Dickson, 2001.3.14, Molecular details of robo2 alleles. Molecular details of robo2 alleles. [FBrf0135264]

Allele Dmel\learobo2-1

Allele Dmel\lea^robo2-1