Allele Dmel\per^Scer\UAS.cYa

General Information
Symbol	Dmel\per^Scer\UAS.cYa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Yang	FlyBase ID	FBal0121609
Feature type	allele	Associated gene	Dmel\per

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Yang and Sehgal, 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of full length per cDNA is governed by Scer\UAS regulatory sequences. (Yang and Sehgal, 2001)
Carried in construct	P{UAS-per.Y} (Stoleru et al., 2007, Zhao et al., 2003, Stoleru et al., 2004, Yang and Sehgal, 2001, Collins et al., 2006, Zheng et al., 2009, Kadener et al., 2008, Kaneko et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	locomotor rhythm defective, with Scer\GAL4^Act5C.PI (Yang and Sehgal, 2001) locomotor rhythm defective, with Scer\GAL4^cry.PZ (Zhao et al., 2003) locomotor rhythm defective, with Scer\GAL4^elav-C155 (Yang and Sehgal, 2001) locomotor rhythm defective, with Scer\GAL4^elav-C155, Scer\GAL80^cry.PS, per⁰¹ (Stoleru et al., 2004) locomotor rhythm defective, with Scer\GAL4^P0.5.Pdf (Collins et al., 2006) locomotor rhythm defective, with Scer\GAL4^tim.PE (Yang and Sehgal, 2001) locomotor rhythm defective, with Scer\GAL4^tim.PE, Scer\GAL80^Pdf.PS (Stoleru et al., 2007)
Phenotype Manifest In

Detailed Description
	Statement Reference Flies expressing per^Scer\UAS.cYa under the control of Scer\GAL4^tim.PE in a Scer\GAL80^Pdf.PS background show rhythmic locomotor activity under constant light conditions, in contrast to wild-type flies, which are uniformly arrhythmic under these conditions. (Stoleru et al., 2007) Flies expressing per^Scer\UAS.cYa, under the control of Scer\GAL4^P0.5.Pdf, exhibit low levels of arrhythmicity (approximately 22.2% versus 13%) although the period length is increased by approximately 1 hour. (Collins et al., 2006) Flies mutant for per⁰¹ expressing per^Scer\UAS.cYa under the control of Scer\GAL4^elav-C155 and repressed by Scer\GAL80^Pdf.PS in Pdf expressing cells, become gradually arrhythmic under DD conditions. These flies however, manifest a wild-type-like anticipation of lights-on as well as lights off peak in LD. (Stoleru et al., 2004) When per^Scer\UAS.cYa is driven by Scer\GAL4^cry.PP no induction of ectopic clocks is seen. A reduction is seen behavioural rhythmicity in DD conditions. (Zhao et al., 2003) When expression is driven in a per⁺ background by Scer\GAL4^elav-C155 or Scer\GAL4^Act5C.PI, arythmicity occurs. Two copies of P{UAS-per.Y} give a stronger effect than one. The effect is weaker with Scer\GAL4^tim.PE and not evident with Scer\GAL4^P2.4.Pdf. (Yang and Sehgal, 2001)
External Data
Linkouts
Interactions
Phenotypic Class
NOT Enhanced by
Statement Reference Scer\GAL4^P0.5.Pdf, per^Scer\UAS.cYa has locomotor rhythm defective phenotype, non-enhanceable by Scer\GAL4^P0.5.Pdf/cry^Scer\UAS.cEa (Collins et al., 2006)
NOT Enhancer of
Statement Reference Scer\GAL4^P0.5.Pdf/per^Scer\UAS.cYa is a non-enhancer of locomotor rhythm defective phenotype of Scer\GAL4^P0.5.Pdf, cry^Scer\UAS.cEa (Collins et al., 2006)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Expression of cyc[Scer\UAS.T:Hsim\VP16] under the control of Scer\GAL4[elav-C155] in a per[01], per[Scer\UAS.cYa] background only shortens the circadian locomotor activity period by 20 minutes, in contrast to the shortening of 2 hours seen when cyc[Scer\UAS.T:Hsim\VP16] is expressed under the control of Scer\GAL4[elav-C155] in a wild-type background. (Kadener et al., 2008) Flies expressing per^Scer\UAS.cYa and cry^Scer\UAS.cEa, under the control of Scer\GAL4^P0.5.Pdf, exhibit low levels of arrhythmicity (approximately 15.7% versus 13%). (Collins et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^elav-C155/per^Scer\UAS.cYa rescues per⁰¹ (Stoleru et al., 2004, Kaneko et al., 2012) Scer\GAL4^tim.PE/per^Scer\UAS.cYa rescues per⁰¹ (Kaneko et al., 2012)
Partially rescues	per^Scer\UAS.cYa, Scer\GAL4^Clk.int1-3, Scer\GAL80^Pdf.PS partially rescues per⁰¹ (Kaneko et al., 2012) per^Scer\UAS.cYa/Scer\GAL4^Clk.int1-3 partially rescues per⁰¹ (Kaneko et al., 2012) Scer\GAL4^elav-C155/per^Scer\UAS.cYa partially rescues per⁰¹ (Yang and Sehgal, 2001, Kadener et al., 2008)
Fails to rescue	Scer\GAL4^elav-C155, per^Scer\UAS.cYa, Scer\GAL80^cry.PS fails to rescue per⁰¹ (Stoleru et al., 2004) Scer\GAL4^elav-C155, per^Scer\UAS.cYa, Scer\GAL80^Pdf.PS fails to rescue per⁰¹ (Stoleru et al., 2004)
Comments	Expression of per[Scer\UAS.cYa] in neuronal cells (under the control of Scer\GAL4[elav-C155]) or in circadian cells (under the control of Scer\GAL4[tim.PE]) rescues daytime temperature preference rhythms in per[01] mutant flies. Expression of per[Scer\UAS.cYa] in M cells, under the control of Scer\GAL4[P2.4.Pdf], fails to rescue daytime temperature preference rhythm under light-dark conditions, although a weaker-amplitude rhythm with an abnormal phase is present in these flies. Expression of per[Scer\UAS.cYa] in both M and E cells with Scer\GAL4[cry.PE] is unable to rescue daytime temperature preference rhythms under light-dark conditions. Expression of per[Scer\UAS.cYa] in the DN2 neurons (under the control of Scer\GAL4[Clk.int1-3]) rescues approximately 46% of the per[01] mutant phenotype for daytime temperature preference rhythm. Expression of per[Scer\UAS.cYa] in the DN2 neurons (under the control of Scer\GAL4[Clk.int1-3] and Scer\GAL80[Pdf.PS]) rescues approximately 66% of the per[01] mutant phenotype for daytime temperature preference rhythm. Expression of per[Scer\UAS.cYa] in DN2 neurons under the control of Scer\GAL4[Clk.int1-3] and Scer\GAL80[Pdf.PS] does not rescue the per[01] locomotor activity phenotypes under either light-dark or temperature cycles. These flies are also arrhythmic in dark-dark conditions. (Kaneko et al., 2012) Expression of per[Scer\UAS.cYa] under the control of Scer\GAL4[elav-C155] can rescue the arrhythmic locomotor activity phenotype of per[01] flies, with 65.2% of the rescued flies being rhythmic for locomotor activity. (Kadener et al., 2008) The degree of rescue of the per⁰¹ locomotor rhythm defects depends to some extent on the site of insertion of the rescuing P{UAS-per.Y} transgene and is maximally >90% and minimally >50%. (Yang and Sehgal, 2001)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	per^Scer\UAS.cYa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Yang (Yang and Sehgal, 2001)
Secondary FlyBase IDs

References ( 8 )
Research paper	Kaneko et al., 2012, Curr. Biol. 22(19): 1851--1857 Circadian rhythm of temperature preference and its neural control in Drosophila. [FBrf0219631] Zheng et al., 2009, J. Neurosci. 29(35): 10920--10927 An isoform-specific mutant reveals a role of PDP1 epsilon in the circadian oscillator. [FBrf0208808] Kadener et al., 2008, PLoS Biol. 6(5): e119 Circadian transcription contributes to core period determination in Drosophila. [FBrf0204901] Stoleru et al., 2007, Cell 129(1): 207--219 The Drosophila circadian network is a seasonal timer. [FBrf0202062] Collins et al., 2006, Curr. Biol. 16(5): 441--449 Drosophila CRYPTOCHROME is a circadian transcriptional repressor. [FBrf0190005] Stoleru et al., 2004, Nature 431(7010): 862--868 Coupled oscillators control morning and evening locomotor behaviour of Drosophila. [FBrf0179740] Zhao et al., 2003, Cell 113(6): 755--766 Drosophila clock can generate ectopic circadian clocks. [FBrf0159721] Yang and Sehgal, 2001, Neuron 29(2): 453--467 Role of molecular oscillations in generating behavioral rhythms in Drosophila. [FBrf0134807]

Allele Dmel\perScer\UAS.cYa

Allele Dmel\per^Scer\UAS.cYa