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General Information
Symbol
Dmel\perUAS.cYa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Yang
FlyBase ID
FBal0121609
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-per
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of full length per cDNA is governed by UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing perScer\UAS.cYa under the control of Scer\GAL4P2.4.Pdf show similar locomotor activity period under free-running conditions (total darkness) as controls but the percentage as well as the rhythmic power of the behavioral oscillations is somewhat decreased.

Overexpression of perScer\UAS.cYa under the control of Scer\GAL4P2.4.Pdf leads to a long period phenotype.

Flies expressing perScer\UAS.cYa under the control of Scer\GAL4tim.PE in a Scer\GAL80Pdf.PS background show rhythmic locomotor activity under constant light conditions, in contrast to wild-type flies, which are uniformly arrhythmic under these conditions.

Flies expressing perScer\UAS.cYa, under the control of Scer\GAL4P0.5.Pdf, exhibit low levels of arrhythmicity (approximately 22.2% versus 13%) although the period length is increased by approximately 1 hour.

Flies mutant for per01 expressing perScer\UAS.cYa under the control of Scer\GAL4elav-C155 and repressed by Scer\GAL80Pdf.PS in Pdf expressing cells, become gradually arrhythmic under DD conditions. These flies however, manifest a wild-type-like anticipation of lights-on as well as lights off peak in LD.

When perScer\UAS.cYa is driven by Scer\GAL4cry.PP no induction of ectopic clocks is seen. A reduction is seen behavioural rhythmicity in DD conditions.

When expression is driven in a per+ background by Scer\GAL4elav-C155 or Scer\GAL4Act5C.PI, arythmicity occurs. Two copies of P{UAS-per.Y} give a stronger effect than one. The effect is weaker with Scer\GAL4tim.PE and not evident with Scer\GAL4P2.4.Pdf.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The loss of rhythmicity in locomotor activity under free-running (constant darkness) conditions characteristic for flies expressing SRm160KK108396 (and UAS-dicer2 to enhance the RNAi efficiency) under the control of Scer\GAL4P2.4.Pdf is significantly improved by co-expression of perScer\UAS.cYa.

Co-expression of perScer\UAS.cYa and shnScer\UAS.cMa under the control of Scer\GAL4P2.4.Pdf results in highly rhythmic flies, with periods close to 24 hours.

Co-expression of perScer\UAS.cYa and timScer\UAS.cYa under the control of Scer\GAL4P2.4.Pdf results in highly rhythmic flies, with periods close to 24 hours.

Expression of perScer\UAS.cYa suppresses the lengthening of the circadian locomotor darkness period seen when Atx2KK100423 is expressed under the control of Scer\GAL4tim.PE.

Expression of cycScer\UAS.T:Hsim\VP16 under the control of Scer\GAL4elav-C155 in a per01, perScer\UAS.cYa background only shortens the circadian locomotor activity period by 20 minutes, in contrast to the shortening of 2 hours seen when cycScer\UAS.T:Hsim\VP16 is expressed under the control of Scer\GAL4elav-C155 in a wild-type background.

Flies expressing perScer\UAS.cYa and cryScer\UAS.cEa, under the control of Scer\GAL4P0.5.Pdf, exhibit low levels of arrhythmicity (approximately 15.7% versus 13%).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of perScer\UAS.cYa in neuronal cells (under the control of Scer\GAL4elav-C155) or in circadian cells (under the control of Scer\GAL4tim.PE) rescues daytime temperature preference rhythms in per01 mutant flies.

Expression of perScer\UAS.cYa in M cells, under the control of Scer\GAL4P2.4.Pdf, fails to rescue daytime temperature preference rhythm under light-dark conditions, although a weaker-amplitude rhythm with an abnormal phase is present in these flies.

Expression of perScer\UAS.cYa in both M and E cells with Scer\GAL4cry.PE is unable to rescue daytime temperature preference rhythms under light-dark conditions.

Expression of perScer\UAS.cYa in the DN2 neurons (under the control of Scer\GAL4Clk.int1-3) rescues approximately 46% of the per01 mutant phenotype for daytime temperature preference rhythm.

Expression of perScer\UAS.cYa in the DN2 neurons (under the control of Scer\GAL4Clk.int1-3 and Scer\GAL80Pdf.PS) rescues approximately 66% of the per01 mutant phenotype for daytime temperature preference rhythm.

Expression of perScer\UAS.cYa in DN2 neurons under the control of Scer\GAL4Clk.int1-3 and Scer\GAL80Pdf.PS does not rescue the per01 locomotor activity phenotypes under either light-dark or temperature cycles. These flies are also arrhythmic in dark-dark conditions.

Expression of perScer\UAS.cYa under the control of Scer\GAL4elav-C155 can rescue the arrhythmic locomotor activity phenotype of per01 flies, with 65.2% of the rescued flies being rhythmic for locomotor activity.

The degree of rescue of the per01 locomotor rhythm defects depends to some extent on the site of insertion of the rescuing P{UAS-per.Y} transgene and is maximally >90% and minimally >50%.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
perScer\UAS.cYa
perUAS.cYa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Yang
Secondary FlyBase IDs
    References (12)