Loss-of-function hth100-1 eye clones exhibit a dorsal-ventral constraint in their phenotypes, where clones in the ventral eye result in eye enlargement or induction of ectopic eyes, whereas in the dorsal eye these clones do not affect the eye fate.
hth100-1 embryos show head defects. In these embryos, A1 is weakly transformed toward A2, but the rest of the abdominal segments have a wild-type pattern of denticle belts. There are no segmental fusions and the thoracic segments are normal except for the rare appearance of a small number of ectopic denticles.
hth100-1 clones induced in the antennal imaginal disc result in the transformation of the antenna towards a leg. hth100-1 clones induced in the wing result in aberrant morphology of the wing hinge.
In approximately one-third of hth100-1 legs the tibia and femur are partially fused.
Mutant embryos show a relatively mild phenotype in the PNS, which consists mainly of dorsal localisation of LCh5 neurons in some of the abdominal segments.