Allele Dmel\WASp^Scer\UAS.cBa

General Information
Symbol	Dmel\WASp^Scer\UAS.cBa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Ben-Yaacov	FlyBase ID	FBal0121872
Feature type	allele	Associated gene	Dmel\WASp

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Ben-Yaacov et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a full length WASp cDNA. (Ben-Yaacov et al., 2001)
Carried in construct	P{UAS-WASp.B} (Tal et al., 2002, Coyle et al., 2004, Ben-Yaacov et al., 2001, Rotkopf et al., 2011, Nahm et al., 2010, Schafer et al., 2007, Khuong et al., 2010, Duboff et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	adult brain, with Scer\GAL4^elav.PU (Duboff et al., 2012) mitochondrion, with Scer\GAL4^elav.PU (Duboff et al., 2012)
Detailed Description
	Statement Reference The mitochondria in the neurons of adult brains expressing WASp[Scer\UAS.cBa] under the control of Scer\GAL4[elav.PU] are frequently elongated compared to controls. (Duboff et al., 2012) As in wild type, flies expressing WASp[Scer\UAS.cBa] under the control of Scer\GAL4[arm.PS] in a WASp[1]/Df(3R)3450 mutant background display elongated cysts and a seminal vesicle that is full of individualised, mature sperm. (Rotkopf et al., 2011) Expression of WASp[Scer\UAS.cBa] under the control of Scer\GAL4[C57] has no effect on bouton number/muscle area or the number of satellite boutons at the third larval instar neuromuscular junction. (Nahm et al., 2010) Expression of WASp[Scer\UAS.cBa] in the mesoderm under the control of Scer\GAL4[twi.PG] does not affect muscle development. (Schafer et al., 2007)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Cip4^{Scer\UAS.N.T:Ivir\HA1}, Scer\GAL4^C57, WASp^Scer\UAS.cBa has neuroanatomy defective \| third instar larval stage phenotype (Nahm et al., 2010)
Phenotype Manifest In
Enhancer of
Statement Reference WASp^Scer\UAS.cBa, Scer\GAL4^elav.PU is an enhancer of adult brain phenotype of Hsap\MAPT^{R406W.Scer\UAS}, Scer\GAL4^elav.PU (Duboff et al., 2012) WASp^Scer\UAS.cBa, Scer\GAL4^elav.PU is an enhancer of mitochondrion phenotype of Hsap\MAPT^{R406W.Scer\UAS}, Scer\GAL4^elav.PU (Duboff et al., 2012)
Other
Statement Reference Cip4^{Scer\UAS.N.T:Ivir\HA1}, Scer\GAL4^C57, WASp^Scer\UAS.cBa has NMJ bouton \| third instar larval stage phenotype (Nahm et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of both Cip4[Scer\UAS.N.T:Ivir\HA1] and WASp[Scer\UAS.cBa] under the control of Scer\GAL4[C57] results in a reduction in bouton number/muscle area and in the number of satellite boutons at the third larval instar neuromuscular junction. (Nahm et al., 2010)
Xenogenetic Interactions
Statement Reference Expression of WASp[Scer\UAS.cBa] enhances the increase in mitochondria length seen when Hsap\MAPT[R406W.Scer\UAS] is expressed under the control of Scer\GAL4[elav.PU]. (Duboff et al., 2012)
Complementation & Rescue Data
Rescues	Scer\GAL4^arm.PS/WASp^Scer\UAS.cBa rescues Df(3R)3450/WASp¹ (Tal et al., 2002) Scer\GAL4^arm.PS/WASp^Scer\UAS.cBa rescues WASp^unspecified (Ben-Yaacov et al., 2001) Scer\GAL4^elav-C155/WASp^Scer\UAS.cBa rescues WASp^unspecified (Ben-Yaacov et al., 2001) Scer\GAL4^twi.PG/WASp^Scer\UAS.cBa rescues Df(3R)3450/WASp^3D3-035 (Schafer et al., 2007) WASp^Scer\UAS.cBa/Scer\GAL4^n-syb.PS rescues Df(3R)3450/WASp¹ (Khuong et al., 2010) WASp^Scer\UAS.cBa/Scer\GAL4^T80 rescues Df(3R)3450/WASp¹ (Tal et al., 2002) WASp^Scer\UAS.cBa/Scer\GAL4^T80 rescues WASp^unspecified (Ben-Yaacov et al., 2001)
Partially rescues	WASp^Scer\UAS.cBa/Scer\GAL4^elav.PLu partially rescues WASp¹ (Coyle et al., 2004)
Fails to rescue	Scer\GAL4^kirre-rP298/WASp^Scer\UAS.cBa fails to rescue Df(3R)3450/WASp^3D3-035 (Schafer et al., 2007)
Comments	Expression of WASp[Scer\UAS.cBa] under the control of Scer\GAL4[n-syb.PS] rescues the neuromuscular junction overgrowth seen in WASp[1]/Df(3R)3450 third instar larvae. (Khuong et al., 2010) Expression of WASp[Scer\UAS.cBa] in WASp[3D3-035]/Df(3R)3450 mutants under the control of Scer\GAL4[twi.PG] restores almost the complete muscle pattern. Expression of WASp[Scer\UAS.cBa] specifically in founder cells (under the control of Scer\GAL4[kirre-rP298]) in WASp[3D3-035]/Df(3R)3450 mutants does not restore a wild-type muscle pattern. Instead muscles look thinner compared to wild-type and some muscles are even missing. (Schafer et al., 2007) Expression of WASp^Scer\UAS.cBa under the control of Scer\GAL4^elav.PLu partially rescues the neuromuscular junction defects of WASp¹ mutants; normalised bouton number, NMJ length and branch number are significantly reduced towards wild-type levels. (Coyle et al., 2004)
Stocks ( 1 )
Bloomington	39724 y¹ w^*; P{UAS-WASp.B}25
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	WASp^Scer\UAS.cBa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Ben-Yaacov (Ben-Yaacov et al., 2001)
Secondary FlyBase IDs

References ( 8 )
Research paper	Duboff et al., 2012, Neuron 75(4): 618--632 Tau Promotes Neurodegeneration via DRP1 Mislocalization In Vivo. [FBrf0219271] Rotkopf et al., 2011, Development 138(13): 2729--2739 The WASp-based actin polymerization machinery is required in somatic support cells for spermatid maturation and release. [FBrf0213892] Khuong et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(40): 17379--17384 WASP is activated by phosphatidylinositol-4,5-bisphosphate to restrict synapse growth in a pathway parallel to bone morphogenetic protein signaling. [FBrf0214154] Nahm et al., 2010, J. Cell Biol. 191(3): 661--675 The Cdc42-selective GAP Rich regulates postsynaptic development and retrograde BMP transsynaptic signaling. [FBrf0212218] Schafer et al., 2007, Dev. Biol. 304(2): 664--674 The Wiskott-Aldrich syndrome protein (WASP) is essential for myoblast fusion in Drosophila. [FBrf0194614] Coyle et al., 2004, Neuron 41(4): 521--534 Nervous wreck, an SH3 adaptor protein that interacts with Wsp, regulates synaptic growth in Drosophila. [FBrf0175021] Tal et al., 2002, Dev. Biol. 243(2): 260--271 Conserved interactions with cytoskeletal but not signaling elements are an essential aspect of Drosophila WASp function. [FBrf0144805] Ben-Yaacov et al., 2001, J. Cell Biol. 152(1): 1--14 Wasp, the Drosophila Wiskott-Aldrich Syndrome gene homologue, is required for cell fate decisions mediated by Notch signaling. [FBrf0132374]

Allele Dmel\WASpScer\UAS.cBa

Allele Dmel\WASp^Scer\UAS.cBa