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General Information
Symbol
Dmel\WASpUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Ben-Yaacov
FlyBase ID
FBal0121872
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-WASp, UAS-Wsp
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a full length WASp cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The mitochondria in the neurons of adult brains expressing WASpScer\UAS.cBa under the control of Scer\GAL4elav.PU are frequently elongated compared to controls.

As in wild type, flies expressing WASpScer\UAS.cBa under the control of Scer\GAL4arm.PS in a WASp1/Df(3R)3450 mutant background display elongated cysts and a seminal vesicle that is full of individualised, mature sperm.

Expression of WASpScer\UAS.cBa under the control of Scer\GAL4C57 has no effect on bouton number/muscle area or the number of satellite boutons at the third larval instar neuromuscular junction.

Expression of WASpScer\UAS.cBa in the mesoderm under the control of Scer\GAL4twi.PG does not affect muscle development.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of both Cip4Scer\UAS.N.T:Ivir\HA1 and WASpScer\UAS.cBa under the control of Scer\GAL4C57 results in a reduction in bouton number/muscle area and in the number of satellite boutons at the third larval instar neuromuscular junction.

Xenogenetic Interactions
Statement
Reference

Expression of WASpScer\UAS.cBa enhances the increase in mitochondria length seen when Hsap\MAPTR406W.Scer\UAS is expressed under the control of Scer\GAL4elav.PU.

Complementation and Rescue Data
Partially rescues
Comments

Expression of WASpScer\UAS.cBa under the control of Scer\GAL4n-syb.PS rescues the neuromuscular junction overgrowth seen in WASp1/Df(3R)3450 third instar larvae.

Expression of WASpScer\UAS.cBa in WASp3D3-035/Df(3R)3450 mutants under the control of Scer\GAL4twi.PG restores almost the complete muscle pattern.

Expression of WASpScer\UAS.cBa specifically in founder cells (under the control of Scer\GAL4kirre-rP298) in WASp3D3-035/Df(3R)3450 mutants does not restore a wild-type muscle pattern. Instead muscles look thinner compared to wild-type and some muscles are even missing.

Expression of WASpScer\UAS.cBa under the control of Scer\GAL4elav.PLu partially rescues the neuromuscular junction defects of WASp1 mutants; normalised bouton number, NMJ length and branch number are significantly reduced towards wild-type levels.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
WASpScer\UAS.cBa
WASpUAS.cBa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Ben-Yaacov
Secondary FlyBase IDs
    References (12)