FlyBase Allele Report: Dmel\WASp[3]

General Information

Symbol

Dmel\WASp³

Species

D. melanogaster

Name

FlyBase ID

FBal0121873

Feature type

allele

Associated gene

Dmel\WASp

Associated Insertion(s)

Carried in Construct

Also Known As

Wsp³

Key Links

Genomic Maps

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Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Johnson et al., 2008)

Mutagen

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

deletion

3R:28,826,022..28,826,032 [-]

Comment:

An 11bp deletion (agtcagtgcgc), which results in frameshift within residue Pro-374 and early translation termination after the addition of 114 novel amino acids. Note that FBrf0194614 reports that WASp³ is a deletion of 13bp extending from 1121-1134bp in WASp³.

(Schejter, 2006.7.3, Ben-Yaacov et al., 2001)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

WASp³ carries an intergenic deletion of 13 bp (1121-1134bp) that results in a frameshift, and subsequently the loss of the VVCA domain.

(Schafer et al., 2007)

An 8 nucleotide deletion (24652013 cggaaaca 24652006), replaced with 4 nucleotides (ggtg). This results in frameshift after residue Pro-309 of the protein and termination after 120 additional residues.

(Schejter, 2006.7.3)

Small intragenic deletion resulting in a predicted frameshift which causes the cytoskeleton-interacting carboxy-terminal domain to be lost.

(Ben-Yaacov et al., 2001)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

actin filament & eye photoreceptor cell | somatic clone

(Zelhof and Hardy, 2004)

adult thorax & microchaeta & external sensory organ precursor cell IIa (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

adult thorax & microchaeta & external sensory organ precursor cell IIb (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

denticle

(Chanut-Delalande et al., 2006)

dorsocentral bristle (with Df(3R)3450)

(Bogdan et al., 2005)

embryonic cardioblast | increased number

(Dondi et al., 2021)

embryonic myoblast

(Kim et al., 2007)

embryonic myoblast (with WASp^3D3-035)

(Schafer et al., 2007)

embryonic peripheral nervous system & external sensory organ | germ-line clone (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

embryonic peripheral nervous system & neuron | supernumerary | germ-line clone (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

external sensory organ | somatic clone

(Benhra et al., 2011)

external sensory organ precursor cell IIa | somatic clone

(Rajan et al., 2009)

external sensory organ precursor cell IIb | somatic clone

(Rajan et al., 2009)

eye disc | somatic clone

(Zelhof and Hardy, 2004)

filamentous actin | embryonic stage

(Dondi et al., 2021)

larval ventral nerve cord | rescuable maternal effect

(Zallen et al., 2002)

microchaeta (with Df(3R)3450)

(Bogdan et al., 2005)

microvillus & eye photoreceptor cell | somatic clone

(Zelhof and Hardy, 2004)

muscle attachment site

(Schafer et al., 2007)

neuron | embryonic stage (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

notopleural bristle (with Df(3R)3450)

(Bogdan et al., 2005)

nucleus | embryonic stage

(Dondi et al., 2021)

primordial germ cell | embryonic stage

(Kim et al., 2021)

primordial germ cell | embryonic stage (with WASp¹)

(Kim et al., 2021)

rhabdomere (with WASp¹)

(Zelhof and Hardy, 2004)

rhabdomere | somatic clone

(Zelhof and Hardy, 2004)

RP2 motor neuron | increased number (with Df(3R)3450)

(Ben-Yaacov et al., 2001)

scutellar bristle (with Df(3R)3450)

(Bogdan et al., 2005)

Detailed Description

Statement

Reference

Homozygous embryos have a wild-type muscle pattern.

(Kaipa et al., 2013)

WASp³ double mutant clones in the adult thorax exhibit external sensory cell loss and an excess of neurons.

(Benhra et al., 2011)

Only 14% of actin foci in embryos maternally and zygotically mutant for WASp³ invade into founder cells, compared to 35% in wild type. The depth of invasion is also reduced.

(Sens et al., 2010)

Within WASp³ pIIa-pIIb cells, an apical actin-rich structure (ARS) is formed as in wild type, but its apical area is markedly reduced and, in about 50% of cases, the stalk of the normal 'umbrella shape' is not formed properly.

(Rajan et al., 2009)

Homozygous embryos derived from females carrying homozygous germline clones show severe myoblast fusion defects.

(Kim et al., 2007)

WASp³ mutants exhibit muscle attachment defects but do not display unfused myoblasts.

WASp^3D3-035/WASp³ transheterozygotes exhibit a myoblast fusion phenotype.

(Schafer et al., 2007)

The cuticles of WASp³ mutants have abnormally thin denticles, which often display bent extremities.

(Chanut-Delalande et al., 2006)

WASp³/Df(3R)3450 mutants show a frequent loss of dorsocentral and scutellar bristles. The notopleural bristles are missing. They also show loss of microchaetae.

(Bogdan et al., 2005)

WASp¹/WASp³ transheterozygotes survive to pharate adults. In these flies, many rhabdomeres do not obtain the normal characteristic oval shape, but rather are pointed and squared, with 2-3% of the mutant rhabdomeres split. The same phenotypes are seen in WASp³ homozygous somatic clones. In these clones F-actin accumulation along the photoreceptor apical surface is delayed and disorganised. At 48 hours there are regions of the photoreceptor cell in which the apical surface has not expanded and/or primordial microvillar projections have not formed. These regions are characterised by little or no F-actin enrichment compared with neighbouring wild-type cells. Ultrastructural analysis at this stage reveals loss of adherens junctions between mutant photoreceptors, no separation or elimination of cell-cell contacts between photoreceptor cells and a lack of primordial microvillar projections. While the initiation of rhabdomere microvilli formation is delayed, WASp³ mutant photoreceptor cells do form microvilli with an actin cytoskeleton core. At 72 hours, the developing mutant rhabdomeres are smaller in size or malformed compared with wild-type.

(Zelhof and Hardy, 2004)

Syncytial blastoderm stage WASp³ germ-line clone embryos have wild-type nuclear morphology and distribution during nuclear division cycles 12-14. There are also no obvious cytoskeletal defects in mutants at these stages. In WASp³/Df(3R)3450 embryos from WASp³ germ-line clone carrying mothers, axon bundles in the ventral nerve cord appear thicker and defasciculated. Most of these embryos contain one to two segments with axon bundles collapsed at the midline (73% of embryos, n = 41). WASp³ mutant germ-lines undergo normal oogenesis and nurse cell development.

(Zallen et al., 2002)

Hemizygotes complete nearly all stages of imaginal development and die as young adults, most commonly failing to eclose from the pupal case. Those that eclose can survive for a few days, but are lethargic and passive in their behaviour. WASp³/Df(3R)3450 embryos derived from WASp³ homozygous female germline clones do not survive. Embryos derived from WASp³ homozygous female germline clones with a wild-type paternal contribution of WASp develop normally, giving rise to viable and fertile adults. WASp³/Df(3R)3450 embryos derived from WASp³ homozygous female germline clones have normal cuticles. The initial selection of sensory organ determination appears to occur normally in these embryos, but they have an excess of neurons (a near doubling of normal numbers) in the mature peripheral nervous system (PNS). A corresponding reduction in the number of PNS nonneuronal support cells is seen; the number of cells expressing shaft and socket cell markers is reduced, although only mild effects on sheath cell numbers are seen. RP2 neuroblasts are duplicated. The mitotic spindles in the notum of WASp³/Df(3R)3450 pupae are positioned within the plane of the epithelium and along the anterior-posterior axis of the fly, as in wild type. The pI division in the lineage of the thoracic microchaetae is normal in WASp³/Df(3R)3450 pupae. However, subsequent events in the differentiation of this sensory organ lineage differ substantially from wild type. Either of the two pI daughter cells may divide after pI (in wild type the anterior pIIb cell always divides before the posterior pIIa cell) and the divisions of both of the pI daughter cells are morphologically identical and resemble the pattern seen in wild-type pIIb cells (both cell divisions are nonplanar and generate two daughter cells of different sizes).

(Ben-Yaacov et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

WASp[+]/WASp³ is an enhancer of abnormal planar polarity phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, cindr^{dsRNA.PC.PD.UAS}

(Johnson et al., 2008)

NOT Enhancer of

Statement

Reference

WASp[+]/WASp³ is a non-enhancer of visible phenotype of Df(3L)Exel8098/Cip4^Δ32

(Fricke et al., 2009)

Suppressor of

Statement

Reference

WASp[+]/WASp³ is a suppressor of abnormal cell shape | extended germ band stage phenotype of upd1^YM55

(Bertet et al., 2009)

WASp³ is a suppressor | partially of visible phenotype of Abi^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^sca-537.4

(Bogdan et al., 2005)

NOT Suppressor of

Statement

Reference

WASp[+]/WASp³ is a non-suppressor of visible phenotype of Df(3L)Exel8098/Cip4^Δ32

(Fricke et al., 2009)

Other

Statement

Reference

Msp300^sz75, WASp³ has increased cell number | embryonic stage phenotype

(Dondi et al., 2021)

Phenotype Manifest In

Enhanced by

Statement

Reference

WASp³/WASp¹ has primordial germ cell | embryonic stage phenotype, enhanceable by PIP5K59B¹⁸

(Kim et al., 2021)

Suppressed by

Statement

Reference

WASp³ has external sensory organ | somatic clone phenotype, suppressible by AP-1μ^SHE-11

(Benhra et al., 2011)

Enhancer of

Statement

Reference

WASp³ is an enhancer of embryonic myoblast phenotype of Vrp1^f06715

(Kaipa et al., 2013)

WASp³ is an enhancer of embryonic myoblast phenotype of Pak3^del

(Duan et al., 2012)

WASp³ is an enhancer of myoblast | embryonic stage phenotype of Arp3^schwachling

(Berger et al., 2008)

WASp[+]/WASp³ is an enhancer of myoblast | embryonic stage phenotype of SCAR^k13811/SCAR^k13811

(Berger et al., 2008)

WASp³ is an enhancer of dorsal acute muscle cell | embryonic stage phenotype of Arp3^schwachling

(Berger et al., 2008)

WASp[+]/WASp³ is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, cindr^{dsRNA.PC.PD.UAS}

(Johnson et al., 2008)

WASp[+]/WASp³ is an enhancer of pigment cell phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, cindr^{dsRNA.PC.PD.UAS}

(Johnson et al., 2008)

WASp[+]/WASp³ is an enhancer of cone cell phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, cindr^{dsRNA.PC.PD.UAS}

(Johnson et al., 2008)

WASp³ is an enhancer of denticle phenotype of f^36a, m¹, sn³

(Chanut-Delalande et al., 2006)

WASp³ is an enhancer of abdominal dorsal trichome phenotype of f^36a, m¹, sn³

(Chanut-Delalande et al., 2006)

NOT Enhancer of

Statement

Reference

WASp[+]/WASp³ is a non-enhancer of wing hair | increased number phenotype of Df(3L)Exel8098/Cip4^Δ32

(Fricke et al., 2009)

Suppressor of

Statement

Reference

WASp[+]/WASp³ is a suppressor of ectoderm | extended germ band stage phenotype of upd1^YM55

(Bertet et al., 2009)

WASp³ is a suppressor | partially of notopleural bristle | increased number phenotype of Abi^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^sca-537.4

(Bogdan et al., 2005)

WASp[+]/WASp³ is a suppressor of microchaeta phenotype of Scer\GAL4^sca.PC, Cyfip^{UAS.Tag:Myr(Src64B)}

(Bogdan et al., 2004)

WASp[+]/WASp³ is a suppressor of macrochaeta phenotype of Hem^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^sca-537.4

(Bogdan and Klambt, 2003)

NOT Suppressor of

Statement

Reference

WASp[+]/WASp³ is a non-suppressor of microchaeta phenotype of Abp1^{UAS.Tag:Myr(Src64B),GFP}, Scer\GAL4^sca.PU

(Koch et al., 2012)

WASp³ is a non-suppressor of tormogen cell | increased number | somatic clone phenotype of AP-1μ^SHE-11

(Benhra et al., 2011)

WASp[+]/WASp³ is a non-suppressor of wing hair | increased number phenotype of Df(3L)Exel8098/Cip4^Δ32

(Fricke et al., 2009)

WASp³/WASp³ is a non-suppressor of larval ventral nerve cord commissure phenotype of Hem^C3-20

(Bogdan and Klambt, 2003)

Other

Statement

Reference

Msp300^sz75, WASp³ has embryonic cardioblast | increased number phenotype

(Dondi et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

WASp³ enhances the myoblast fusion defects seen in Vrp1^f06715 embryos.

(Kaipa et al., 2013)

Pak3^del WASp³ double mutant embryos have a more severe myoblast fusion defect than either single mutant.

(Duan et al., 2012)

Heterozygosity for WASp³ does not suppress the split microchaete phenotype caused by expression of Abp1^{Scer\UAS.T:Myr-Src64B,T:Avic\GFP} under the control of Scer\GAL4^sca.PU.

(Koch et al., 2012)

AP-47^SHE-11 suppresses the external sensory cell loss and excess neurons seen in WASp³ mutant thorax clones, instead producing the extra socket cell phenotype seen in AP-47^SHE-11 mutants alone.

(Benhra et al., 2011)

Expression of psidin^Scer\UAS.cKa under the control of Scer\GAL4^slbo.2.6 in a WASp³ heterozygous background has little or no effect on border cell migration.

(Kim et al., 2011)

The reduced ectodermal apical cell surface size observed in os^upd-4 mutant embryos at mid germ-band extension is rescued in os^upd-4; WASp³/+ embryos.

(Bertet et al., 2009)

WASp³/+ has no effect on the fraction of wing cells that have multiple wing hairs in Cip4^Δ32/Df(3L)Exel8098 adults.

(Fricke et al., 2009)

Myoblast fusion does not occur in DA1 muscles in Arp66B^schwachling, WASp³ double mutants.

A WASp³/+ background intensifies the appearance of unfused myoblasts in SCAR^k13811 mutants.

(Berger et al., 2008)

A WASp³ heterozygous background enhances the patterning defects found in Scer\GAL4^GMR.PF>cindr^{dsRNA.PC.PD.Scer\UAS} mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

(Johnson et al., 2008)

sn³, m¹, f^36a; sha^V15 and sn³, m¹, f^36a; WASp³ quadruple mutants show further impairment to denticle formation compared to sn³, m¹, f^36a triple mutants, with mutants exhibiting regions of naked cuticle where denticles lie in wild-type animals.

The dorsal hairs on the abdominal segments of sn³, m¹, f^36a triple mutants are severly reduced in size, and in some cases, hairs are abrogated leaving abnormal naked regions. This phenotype is more severe in sn³, m¹, f^36a; WASp³ quadruple mutants.

(Chanut-Delalande et al., 2006)

The extra notopleural bristles induced by expression of Abi^{Scer\UAS.T:Myr1} under the control of Scer\GAL4^sca-537.4 can be partially suppressed by WASp³.

(Bogdan et al., 2005)

The short and split microchaetae phenotype of Sra-1^{Scer\UAS.T:Myr1}; Scer\GAL4^sca.PC flies is suppressed by WASp³/+.

(Bogdan et al., 2004)

The bristle phenotype due to Hem^{Scer\UAS.T:Myr1}; Scer\GAL4^sca-537.4 is dominantly suppressed by WASp³.

(Bogdan and Klambt, 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

WASp¹

(Zelhof and Hardy, 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

39725

e¹ WASp³/TM6B, Tb¹

Availability

Stated to be lost.

(Schejter, 2006.7.3)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

WASp³

(Dondi et al., 2021, Trylinski and Schweisguth, 2019, Benhra et al., 2011, Berger et al., 2008, Johnson et al., 2008, Schejter, 2006.7.3)

Wasp³

(Sens et al., 2010, Kim et al., 2007)

Wsp³

(Ng and Luo, 2004, Zallen et al., 2002, Ben-Yaacov et al., 2001)

wasp³

(Kim et al., 2021, Zhang et al., 2020, Kaipa et al., 2013, Duan et al., 2012, Koch et al., 2012, Stephan et al., 2011, Bertet et al., 2009, Fricke et al., 2009, Schafer et al., 2007, Bogdan et al., 2005)

wasp^Δ3

(Babatz et al., 2018)

wsp³

(Gault et al., 2012, Kim et al., 2011, Warner and Longmore, 2009, Corrigall et al., 2007, Chanut-Delalande et al., 2006, Bogdan et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (31)

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