Allele Dmel\Uba2^{C175S.Scer\UAS}

General Information
Symbol	Dmel\Uba2^{C175S.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0121879
Feature type	allele	Associated gene	Dmel\Uba2

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Long and Griffith, 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a mutated form of Uba2. (Long and Griffith, 2000)
Carried in construct	P{UAS-Uba2.C175S} (Chan et al., 2002, Long and Griffith, 2000, Arya et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell death \| temperature conditional, with Scer\GAL4^GMR.PF (Chan et al., 2002) visible, with Scer\GAL4^GMR.PF (Long and Griffith, 2000)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PF (Long and Griffith, 2000) eye \| temperature conditional, with Scer\GAL4^GMR.PF (Chan et al., 2002) ommatidium, with Scer\GAL4^GMR.PF (Long and Griffith, 2000) ommatidium \| temperature conditional, with Scer\GAL4^GMR.PF (Chan et al., 2002) pigment cell, with Scer\GAL4^GMR.PF (Long and Griffith, 2000)
Detailed Description
	Statement Reference Overexpression of Uba2[C175S.Scer\UAS] driven by Scer\GAL4[GMR.PF] results in a variable slightly glossy, rough eye phenotype, sometimes with loss of pigmentation. This is temperature-dependent. (Chan et al., 2002) Expression of one copy of Uba2^{C175S.Scer\UAS} under the control of Scer\GAL4^elav-C155 generally results in the production of apparently normal adult progeny with no or very mild lethality at 29^oC. P{UAS-Uba2.C175S} lines expressing higher levels of Uba2^{C175S.Scer\UAS} result in higher lethality (approximately 50% for P{UAS-Uba2.C175S}117F and 100% for P{UAS-Uba2.C175S}92E). Animals expressing high levels of Uba2^{C175S.Scer\UAS} under the control of Scer\GAL4^GMR.PF have a rough, depigmented eye. (Long and Griffith, 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Uba2^{C175S.Scer\UAS} has increased cell death \| temperature conditional phenotype, enhanceable by Hsap\AR^{Q112.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Chan et al., 2002)
NOT suppressed by
Statement Reference Hsap\AR^{Q112.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Uba2^{C175S.Scer\UAS} has increased cell death \| temperature conditional phenotype, non-suppressible by Hsap\HSPA1L^Scer\UAS.cWa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Chan et al., 2002)
Enhancer of
Statement Reference Uba2^{C175S.Scer\UAS}, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PU (Arya et al., 2010) Uba2^{C175S.Scer\UAS}, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Hsp60D^{dsRNA.Sym.Scer\UAS}/Hsp60D^{a.Sym.Scer\UAS}, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} (Arya et al., 2010) Uba2^{C175S.Scer\UAS}/Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} (Arya et al., 2010)
NOT Enhancer of
Statement Reference Uba2^{C175S.Scer\UAS}, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Hsp60D^{dsRNA.Sym.Scer\UAS}/Hsp60D^{a.Sym.Scer\UAS}, Scer\GAL4^GMR.PU (Arya et al., 2010)
NOT Suppressor of
Statement Reference Uba2^{C175S.Scer\UAS}, Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Hsp60D^{dsRNA.Sym.Scer\UAS}/Hsp60D^{a.Sym.Scer\UAS}, Scer\GAL4^GMR.PU (Arya et al., 2010)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Uba2^{C175S.Scer\UAS} has eye \| temperature conditional phenotype, enhanceable by Hsap\AR^{Q112.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Chan et al., 2002) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Uba2^{C175S.Scer\UAS} has ommatidium \| temperature conditional phenotype, enhanceable by Hsap\AR^{Q112.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Chan et al., 2002)
NOT suppressed by
Statement Reference Hsap\AR^{Q112.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Uba2^{C175S.Scer\UAS} has eye \| temperature conditional phenotype, non-suppressible by Hsap\HSPA1L^Scer\UAS.cWa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Chan et al., 2002) Hsap\AR^{Q112.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Uba2^{C175S.Scer\UAS} has eye phenotype, non-suppressible by Hsap\HSPA1L^Scer\UAS.cWa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Chan et al., 2002) Hsap\AR^{Q112.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Uba2^{C175S.Scer\UAS} has ommatidium \| temperature conditional phenotype, non-suppressible by Hsap\HSPA1L^Scer\UAS.cWa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Chan et al., 2002)
Enhancer of
Statement Reference Uba2^{C175S.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\AR^{Q112.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Chan et al., 2002) Uba2^{C175S.Scer\UAS}, Scer\GAL4^GMR.PU is an enhancer of ommatidium phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PU (Arya et al., 2010) Uba2^{C175S.Scer\UAS}, Scer\GAL4^GMR.PU is an enhancer of ommatidium phenotype of Hsp60D^{dsRNA.Sym.Scer\UAS}/Hsp60D^{a.Sym.Scer\UAS}, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} (Arya et al., 2010) Uba2^{C175S.Scer\UAS}/Scer\GAL4^GMR.PU is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} (Arya et al., 2010)
NOT Enhancer of
Statement Reference Uba2^{C175S.Scer\UAS}, Scer\GAL4^GMR.PU is a non-enhancer of ommatidium phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Hsp60D^{dsRNA.Sym.Scer\UAS}/Hsp60D^{a.Sym.Scer\UAS}, Scer\GAL4^GMR.PU (Arya et al., 2010)
NOT Suppressor of
Statement Reference Uba2^{C175S.Scer\UAS}, Scer\GAL4^GMR.PU is a non-suppressor of ommatidium phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Hsp60D^{dsRNA.Sym.Scer\UAS}/Hsp60D^{a.Sym.Scer\UAS}, Scer\GAL4^GMR.PU (Arya et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference The eye phenotype resulting from simultaneous expression of Hsap\MJD[tr.Q78.Scer\UAS.T:Ivir\HA1] and Hsp60D[dsRNA.Sym.Scer\UAS] via Scer\GAL4[GMR.PU] does not appear to be affected by co-expression of Uba2[C175S.Scer\UAS]. The Hsp60D[dsRNA.Sym.Scer\UAS]-mediated suppression of the eye phenotype resulting from the expression of Zzzz\CAG[127Q.Scer\UAS.T:Ivir\HA1] driven by Scer\GAL4[GMR.PU] is less pronounced in the presence of Uba2[C175S.Scer\UAS]. Simultaneous expression of Uba2[C175S.Scer\UAS] and Hsap\MJD[tr.Q78.Scer\UAS.T:Ivir\HA1] via Scer\GAL4[GMR.PU] enhances eye degradation, resulting in more glazed eyes with little pigmentation. Simultaneous expression of Uba2[C175S.Scer\UAS] and Zzzz\CAG[127Q.Scer\UAS.T:Ivir\HA1] via Scer\GAL4[GMR.PU] enhances the eye degeneration phenotype. (Arya et al., 2010) Co-expression of Uba2[C175S.Scer\UAS] with Hsap\AR[Q112.Scer\UAS.T:Ivir\HA1] (both under the control of Scer\GAL4[GMR.PF]) results in a constant enhancement of the eye degeneration, as illustrated by severe loss of pigmentation. Co-expression of Uba2[C175S.Scer\UAS] and Hsap\HSPA1L[Scer\UAS.cWa] with Hsap\AR[Q112.Scer\UAS.T:Ivir\HA1] (all under the control of Scer\GAL4[GMR.PF]) fails to suppress the enhancement of the eye degeneration phenotype found in Uba2[C175S.Scer\UAS] ; Hsap\AR[Q112.Scer\UAS.T:Ivir\HA1] flies. (Chan et al., 2002)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Uba2^{C175S.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Arya et al., 2010, Ann. Neurosci. 17(1): 8--17 Hsp60D - A novel modifier of polyglutamine-mediated neuro degeneration in Drosophila. [FBrf0211649] Chan et al., 2002, Hum. Mol. Genet. 11(23): 2895--2904 Genetic modulation of polyglutamine toxicity by protein conjugation pathways in Drosophila. [FBrf0152073] Long and Griffith, 2000, J. Biol. Chem. 275(52): 40765--40776 Identification and characterization of a SUMO-1 conjugation system that modifies neuronal calcium/calmodulin-dependent protein kinase II in Drosophila melanogaster. [FBrf0132366]

Allele Dmel\Uba2C175S.Scer\UAS

Allele Dmel\Uba2^{C175S.Scer\UAS}