Allele Dmel\tum^Scer\UAS.cSa

General Information
Symbol	Dmel\tum^Scer\UAS.cSa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Sotillos	FlyBase ID	FBal0122004
Feature type	allele	Created / Updated	2006-08-20/2007-07-09
Associated gene	Dmel\tum

Allele class
Mutagen	in vitro construct \| regulatory fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Sotillos and Campuzano, 2000)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS governs the expression of RacGAP50C cDNA. (Sotillos and Campuzano, 2000)
Assay mode	In transgenic Drosophila (intraspecific) (Sotillos and Campuzano, 2000)
Carried in construct	P{UAS-RacGAP.S} (Jones, 2005, Sotillos and Campuzano, 2000)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	wing vein, with Scer\GAL4^Bx-MS1096 (Sotillos and Campuzano, 2000) wing, with Scer\GAL4^Bx-MS1096 (Sotillos and Campuzano, 2000) wing vein, with Scer\GAL4^C-765 (Sotillos and Campuzano, 2000) wing, with Scer\GAL4^C-765 (Sotillos and Campuzano, 2000) wing vein, with Scer\GAL4^en-e16E (Sotillos and Campuzano, 2000) wing, with Scer\GAL4^en-e16E (Sotillos and Campuzano, 2000) sensillum campaniformium, with Scer\GAL4^en-e16E (Sotillos and Campuzano, 2000)
Detailed Description
	Statement Reference When expression is driven by Scer\GAL4^Bx-MS1096 or Scer\GAL4^C-765 the corrugation of the adult vein is eliminated, and the intervein distance decreased. When expression is driven by Scer\GAL4^en-e16E the adult veins are thinned, the wings are reduced in size and the sensory organs reduced in number. (Sotillos and Campuzano, 2000)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference tum^Scer\UAS.cSa, Scer\GAL4^sd-SG29.1 is an enhancer of visible phenotype of Rac1^N17.Scer\UAS, Scer\GAL4^sd-SG29.1 (Sotillos and Campuzano, 2000)
Suppressor of
Statement Reference tum^Scer\UAS.cSa, Scer\GAL4^en-e16E is a suppressor \| partially of cell death increase phenotype of Hsap\CDKN1A^Scer\UAS.cHa, Scer\GAL4^en-e16E (Sotillos and Campuzano, 2000) tum^Scer\UAS.cSa, Scer\GAL4^en-e16E is a suppressor \| partially of tissue polarity defective phenotype of Hsap\CDKN1A^Scer\UAS.cHa, Scer\GAL4^en-e16E (Sotillos and Campuzano, 2000) tum^Scer\UAS.cSa, Scer\GAL4^en-e16E is a suppressor \| partially of visible phenotype of Hsap\CDKN1A^Scer\UAS.cHa, Scer\GAL4^en-e16E (Sotillos and Campuzano, 2000) tum^Scer\UAS.cSa, Scer\GAL4^sd-SG29.1 is a suppressor of lethal phenotype of Rac1^Scer\UAS.cLa, Scer\GAL4^sd-SG29.1 (Sotillos and Campuzano, 2000)
Phenotype Manifest In
Enhancer of
Statement Reference tum^Scer\UAS.cSa, Scer\GAL4^sd-SG29.1 is an enhancer of wing phenotype of Rac1^N17.Scer\UAS, Scer\GAL4^sd-SG29.1 (Sotillos and Campuzano, 2000)
Suppressor of
Statement Reference tum^Scer\UAS.cSa, Scer\GAL4^en-e16E is a suppressor \| partially of wing & cell phenotype of Hsap\CDKN1A^Scer\UAS.cHa, Scer\GAL4^en-e16E (Sotillos and Campuzano, 2000) tum^Scer\UAS.cSa, Scer\GAL4^en-e16E is a suppressor \| partially of wing & trichome phenotype of Hsap\CDKN1A^Scer\UAS.cHa, Scer\GAL4^en-e16E (Sotillos and Campuzano, 2000) tum^Scer\UAS.cSa, Scer\GAL4^en-e16E is a suppressor \| partially of wing phenotype of Hsap\CDKN1A^Scer\UAS.cHa, Scer\GAL4^en-e16E (Sotillos and Campuzano, 2000)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^en-e16E/tum^Scer\UAS.cSa rescues tum^{ΔE1E.Scer\UAS} (Sotillos and Campuzano, 2000)
Partially rescues	Scer\GAL4^e22c/tum^Scer\UAS.cSa partially rescues tum^AR2 (Jones, 2005) Scer\GAL4^en-e16E/tum^Scer\UAS.cSa partially rescues tum^{ΔE1E.Scer\UAS}/Df(2R)CX1 (Sotillos and Campuzano, 2000)
Comments	Expression of RacGAP50C^Scer\UAS.cSa under the control of Scer\GAL4^e22c partially rescues the lethality and completely rescues the cuticle patterning defects of RacGAP50C^AR2 embryos. (Jones, 2005)
Stocks ( 0 )

Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	RacGAP50C^Scer\UAS.cSa RacGAP^Scer\UAS.cSa (Sotillos and Campuzano, 2000, ) tum^Scer\UAS.cSa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Sotillos (Sotillos and Campuzano, 2000)
Secondary FlyBase IDs

References ( 2 )
Research paper	Jones, 2005, Genetics 169(4): 2075--2086 RacGap50C negatively regulates wingless pathway activity during Drosophila embryonic development. [FBrf0188508] Sotillos and Campuzano, 2000, Development 127(24): 5427--5438 DRacGAP, a novel Drosophila gene, inhibits EGFR/Ras signalling in the developing imaginal wing disc. [FBrf0131402]

Allele Dmel\tumScer\UAS.cSa

Allele Dmel\tum^Scer\UAS.cSa