Allele Dmel\Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0122050
Feature type	allele	Associated gene	Dmel\Nc

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Quinn et al., 2000) in vitro construct - regulatory fusion (Quinn et al., 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of an inactive form of Nc tagged with T:Avic\GFP^EGFP. (Quinn et al., 2000)
Carried in construct	P{UAS-Nc.EGFP.C318G} (Martin and Baehrecke, 2004, Quinn et al., 2000, Koto et al., 2009, Williams et al., 2006)
Tagged with	T:Avic\GFP^EGFP (Quinn et al., 2000, Martin and Baehrecke, 2004)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective, with Scer\GAL4⁴⁷⁷ (Williams et al., 2006)
Phenotype Manifest In
	dendrite, with Scer\GAL4⁴⁷⁷ (Williams et al., 2006) dorsal multidendritic neuron ddaC, with Scer\GAL4⁴⁷⁷ (Williams et al., 2006)
Detailed Description
	Statement Reference Overexpression of Nc[ΔN.C-A.Scer\UAS] in the ddaC neurons under the control of Scer\GAL4[477] results in a marked suppression of dendritic pruning. At 14 hours after pupal formation, the dendrites are detached from the soma but still largely cover the same 'footprint' as in third instar larvae. The morphology of detached branches varies: some show proximal swellings, whereas others are thinned and beaded along their entire length. (Williams et al., 2006) Expression of Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PF has little effect on the normal pattern of dying cells in the eye imaginal disc. No death during the pupal stage is seen in animals expressing Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PF and adult flies have normal eyes. (Quinn et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Statement Reference Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^sca-P309 is an enhancer of macrochaeta phenotype of Scer\GAL4^sca-P309, ik2^{dsRNA.Scer\UAS} (Koto et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Nc[C318G.Scer\UAS.T:Avic\GFP-EGFP] in Scer\GAL4[sca-P309]; ik2[dsRNA.Scer\UAS] flies significantly enhances the short-bristle phenotype. (Koto et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP} Nc^{C318G.Scer\UAS.T:Avic\GFP-F64L.S65T}
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Koto et al., 2009, J. Cell Biol. 187(2): 219--231 Temporal regulation of Drosophila IAP1 determines caspase functions in sensory organ development. [FBrf0209002] Williams et al., 2006, Nat. Neurosci. 9(10): 1234--1236 Local caspase activity directs engulfment of dendrites during pruning. [FBrf0192062] Martin and Baehrecke, 2004, Development 131(2): 275--284 Caspases function in autophagic programmed cell death in Drosophila. [FBrf0167528] Quinn et al., 2000, J. Biol. Chem. 275(51): 40416--40424 An essential role for the caspase dronc in developmentally programmed cell death in Drosophila. [FBrf0132365]

Allele Dmel\NcC318G.Scer\UAS.T:Avic\GFP-EGFP

Allele Dmel\Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP}