FlyBase Allele Report: Dmel\EcR[A.UAS]

General Information

Symbol

Dmel\EcR^A.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0122214

Feature type

allele

Associated gene

Dmel\EcR

Associated Insertion(s)

Carried in Construct

P{UAS-EcR.A}

Also Known As

UAS-EcR-A, UAS-EcRA, UAS-EcR.A, EcR-A

Key Links

Transgenic product class

cDNA

(Lee et al., 2000)

wild_type

(Lee et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Lee et al., 2000)

wild_type

(Lee et al., 2000)

Mutagen

in vitro construct

(Lee et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-EcR.A}

Cytology

Description

UASt sequences drive expression of a 3.26 kb EcoRI cDNA fragment containing the EcR-A isoform.

(Lee et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

EcR

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal sleep, with Scer\GAL4^{Mef2.247.Switch}

(Ishimoto and Kitamoto, 2010)

lethal, with Scer\GAL4^Act5C.PI

(Cherbas et al., 2003)

partially lethal, with Scer\GAL4^en-e16E

(Schubiger et al., 2003)

partially lethal - majority die, with Scer\GAL4^phtm.PU

(Gradilla et al., 2011)

Phenotype Manifest In

female germline cell | increased number, with Scer\GAL4^bab1-Pgal4-2

(König et al., 2011)

germarium cap cell | increased number, with Scer\GAL4^bab1-Pgal4-2

(König et al., 2011)

prepupal cuticle, with Scer\GAL4^en-e16E

(Schubiger et al., 2003)

wing vein, with Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

Detailed Description

Statement

Reference

Expression of EcR^A.Scer\UAS in the developing wing under the control of Scer\GAL4^Bx-MS1096 results in mild ectopic wing vein formation.

(Kleefstra et al., 2012)

The number of niche cap cells and the number of germline cells containing a single spectrosome (SSCs) per germarium is increased in females expressing EcR^A.Scer\UAS under the control of Scer\GAL4^bab1-Pgal4-2.

(König et al., 2011)

Expression of EcR^A.Scer\UAS under the control of Scer\GAL4^{Mef2.247.Switch} results in an increase in the total amount of sleep during the day and at night compared to controls. Average sleep bout duration and daytime waking activity are also increased.

(Ishimoto and Kitamoto, 2010)

Expression of EcR^A.Scer\UAS under the control of Scer\GAL4^en-e16E results in some decrease in the hatching rate, but no lethality during metamorphosis; 78% of eggs laid eclose as adults. Prepupae expressing EcR^A.Scer\UAS under the control of Scer\GAL4^en-e16E have reduced tanning in the cuticle overlying posterior cells, resulting in puparia with a striped appearance.

(Schubiger et al., 2003)

Expression of EcR^A.Scer\UAS when driven by Scer\GAL4^Tab2-201Y does not result in any detectable abnormalities.

(Lee et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^fru.P1.D/EcR^A.UAS is a suppressor | partially of abnormal courtship behavior phenotype of Ecol\lexA^VP16.Ilp2.PL, wake^RNAi.lexAop

(Chen et al., 2022)

Scer\GAL4^Or67d.PU/EcR^A.UAS is a suppressor | partially of abnormal courtship behavior phenotype of Ecol\lexA^VP16.Ilp2.PL, wake^RNAi.lexAop

(Chen et al., 2022)

EcR^A.UAS, Scer\GAL4^en-e16E is a suppressor of visible phenotype of Scer\GAL4^en-e16E, pzg^RNAi.UAS

(Kugler et al., 2011)

Other

Statement

Reference

EcR^A.UAS, Scer\GAL4^phtm.PU, ari-1^UAS.cFa has viable phenotype

(Gradilla et al., 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

EcR^A.UAS, Scer\GAL4^Bx-MS1096 is an enhancer of wing vein phenotype of G9a^UAS.cKa, Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

Suppressor of

Statement

Reference

EcR^A.UAS, Scer\GAL4^en-e16E is a suppressor of wing phenotype of Scer\GAL4^en-e16E, pzg^RNAi.UAS

(Kugler et al., 2011)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Tab2-201Y/EcR^A.UAS is a non-suppressor of adult mushroom body gamma-lobe | somatic clone phenotype of babo⁹

(Zheng et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of EcR^A.Scer\UAS enhances the G9a^Scer\UAS.cKa wing vein phenotype.

(Kleefstra et al., 2012)

Co-expression of EcR^A.Scer\UAS significantly suppresses the small wing phenotype caused by expression of pzg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en-e16E.

(Kugler et al., 2011)

The addition of EcR^A.Scer\UAS driven by Scer\GAL4^Tab2-201Y partially rescues the gamma-lobe phenotype.

(Zheng et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Partially rescues

Scer\GAL4^CCAP.PP/EcR^A.UAS partially rescues EcR^{B1-ΔC655.W650A.UAS}

(Lee et al., 2019)

Scer\GAL4^c42/EcR^A.UAS partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

Scer\GAL4^ETH.Switch/EcR^A.UAS partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Gauthier et al., 2012)

Scer\GAL4^Sgs3.PD/EcR^A.UAS partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Cherbas et al., 2003)

Scer\GAL4^dpp.blk1/EcR^A.UAS partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Cherbas et al., 2003)

Scer\GAL4^FMRFa.PS/EcR^A.UAS partially rescues EcR⁹⁹/EcR³¹

(Schubiger et al., 2003)

Fails to rescue

Scer\GAL4^MsrA.602/EcR^A.UAS fails to rescue EcR^{B1-ΔC655.F645A.UAS}

(Cherbas et al., 2003)

Scer\GAL4^slbo.2.6/EcR^A.UAS fails to rescue EcR^{B1-ΔC655.F645A.UAS}

(Cherbas et al., 2003)

Scer\GAL4^Tab2-201Y/EcR^A.UAS fails to rescue EcR³¹/EcR^W53st

(Lee et al., 2000)

Comments

Expressing EcR^A.UAS under the control of Scer\GAL4^CCAP.PP partially rescues both larval CCAP neuronal death and the reduced size of pupal legs and wings, while fully rescuing the head eversion phenotype induced by expression of EcR^{B1-ΔC655.W650A.UAS} alone.

(Lee et al., 2019)

Co-expression of EcR^A.Scer\UAS postpones larval lethality from the 1st instar to the 2nd/3rd instar transition, but results in little-to-no rescue of the Malpighian tubule phenotypes of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^c42. Cuticle preparations of these larvae show molting defects.

(Gautam et al., 2015)

Expression of EcR^A.Scer\UAS under the control of Scer\GAL4^ETH.Switch in the presence of RU486 partially rescues the lethality and failure of ecdysis phenotypes seen in larvae expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^ETH.Switch in the presence of RU486.

(Gauthier et al., 2012)

The defects in midgut morphogenesis which are seen in embryos expressing EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^twi.PG are rescued by co-expression of EcR^A.Scer\UAS.

The defects in tracheal morphogenesis which are seen in embryos expressing EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^btl.PS are rescued by co-expression of EcR^A.Scer\UAS.

The embryonic defects caused by expression or EcR^{B1-ΔC655.W650A.Scer\UAS} under the control of Scer\GAL4^69B are rescued by co-expression of EcR^A.Scer\UAS.

(Chavoshi et al., 2010)

Co-expression of EcR^A.Scer\UAS rescues the lethality and eye defects caused by expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^GMR.PF. Co-expression of EcR^A.Scer\UAS rescues to normal viability and normal morphology animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Lsp2.PH or Scer\GAL4^Eh.0.8. Co-expression of EcR^A.Scer\UAS rescues to nearly complete viability animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^dpp.blk1. Co-expression of EcR^A.Scer\UAS rescues to normal viability and normal morphology animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Ser.PGF. Co-expression of EcR^A.Scer\UAS partially rescues the glue secretion and puffing defects of animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Sgs3.PD. Co-expression of EcR^A.Scer\UAS does not rescue the border cell migration defects, chorion fragility or fertility of females expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^slbo.2.6, and slightly enhances the phenotype, decreasing egg yield and increasing egg fragility.

(Cherbas et al., 2003)

The failure of pruning of the larval Tv-neurons seen in EcR³¹/EcR⁹⁹ animals is slightly rescued by expression of EcR^A.Scer\UAS under the control of Scer\GAL4^Fmrf.PS.

(Schubiger et al., 2003)

EcR^B2.Scer\UAS when driven by Scer\GAL4^Tab2-201Y fails to rescue the Mushroom body γ neuron phenotypes.

(Lee et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6470

w^*; P{UAS-EcR.A}3a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

EcR^A.Scer\UAS

EcR^A.UAS

Name Synonyms

Secondary FlyBase IDs

References (29)

Report Sections

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