Allele Avic\GFP^ECFP.3xP3

General Information
Symbol	Avic\GFP^ECFP.3xP3	Species	A. victoria
Name		FlyBase ID	FBal0122323
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Avic\GFP

Allele class
Mutagen	in vitro construct \| regulatory fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Horn and Wimmer, 2000)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: The "3xP3" promoter sequence (an artificial promoter containing 3 Mmus\Pax6 homodimer binding sites) drives expression of the "ECFP" form ("cyan GFP") of Avic\GFP. (Horn and Wimmer, 2000)
Assay mode	In transgenic Drosophila (allele of foreign species in genome of drosophilid) (Horn and Wimmer, 2000)
Carried in construct	PBac{3xP3-ECFPafm} (Horn and Wimmer, 2000) PBac{3xP3-ECFPaf} (Horn and Wimmer, 2000) Herm{3xP3-ECFPaf} (Horn and Wimmer, 2000) M{3xP3-ECFPafm} (Horn and Wimmer, 2000) M{3xP3-ECFPaf} (Horn and Wimmer, 2000) P{3xP3-ECFPaf} (Horn and Wimmer, 2000) Herm{3xP3-ECFP} (Horn and Wimmer, 2000) P{BLUEi} (Nagel et al., 2002) PBac{3xP3-ECFP,hsp70-Hermes} (Horn et al., 2003) Herm{3xP3-ECFP,αtub-piggyBacK10} (Hacker et al., 2003, Horn et al., 2003) PBac{3xP3-ECFP,UASp-lacZ-K10} (Horn et al., 2003) PBac{3xP3-ECFP,TRE-EYFP-SV40} (Horn et al., 2003) PBac{3xP3-ECFP,TRE-lacZ-SV40} (Horn et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Avic\GFP^ECFP.3xP3
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Hacker et al., 2003, Proc. Natl. Acad. Sci. USA 100(13): 7720--7725 piggyBac-based insertional mutagenesis in the presence of stably integrated P elements in Drosophila. [FBrf0159706] Horn et al., 2003, Genetics 163(2): 647--661 piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics. [FBrf0158985] Nagel et al., 2002, Dev. Genes Evol. 212(2): 93--98 Green fluorescent protein as a convenient and versatile marker for studies on functional genomics in Drosophila. [FBrf0146975] Horn and Wimmer, 2000, Dev. Genes Evol. 210(12): 630--637 A versatile vector set for animal transgenesis. [FBrf0134553]

Allele Avic\GFPECFP.3xP3

Allele Avic\GFP^ECFP.3xP3