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General Information
Symbol
Dmel\sqhE20.E21
Species
D. melanogaster
Name
FlyBase ID
FBal0122886
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
sqhEE, sqhE20E21, sqhE20E21, sqh-E20E21
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A mutant form of sqh is expressed under the control of its own promoter; carries the amino acid replacements T20E and S21E.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

sqhE20.E21/+ significantly reduces egg length, shows increased nurse cell-to-oocyte membrane collapse and high abnormal accumulation of F-actin in nurse cells of stage 9/10 egg chambers compared to controls.

Expression of sqhE20.E21 rescues the para-segmental boundary defects found in Y-27632-treated embryos.

Expression of one copy of the sqhE20.E21 transgene has no effect on axial expansion in wild-type flies.

The addition of sqhE20.E21 rescues the germ band extension defects seen in embryos injected with Y-27632 (a specific inhibitor of rok).

One copy of sqhE20.E21 in a wild-type background has no effect on eye development.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Enhancer of
Suppressor of
Statement
Reference

sqhE20.E21 is a suppressor of lethal phenotype of Drakdel/DrakKO, rok[+]/Rok2

sqhE20.E21 is a suppressor of visible phenotype of DrakKO

sqhE20.E21 is a suppressor of visible phenotype of flw1, pucA251.1F3/puc[+]

sqhE20.E21 is a suppressor | partially of visible | recessive phenotype of dsh1

NOT Suppressor of
Statement
Reference

sqhE20.E21 is a non-suppressor of lethal | male phenotype of flwG0172/flwG0172

sqhE20.E21 is a non-suppressor of lethal | male phenotype of flw6

sqhE20.E21 is a non-suppressor of planar polarity defective phenotype of dsh1

Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
Enhancer of
Suppressor of
Statement
Reference

sqhE20.E21 is a suppressor of wing phenotype of DrakKO

sqhE20.E21 is a suppressor of wing phenotype of flw1, pucA251.1F3/puc[+]

sqhE20.E21/sqh[+] is a suppressor of eye phenotype of Scer\GAL4ey.PB, stidsRNA.Sym.UAS

sqhE20.E21 is a suppressor of wing hair | supernumerary phenotype of Rok2

NOT Suppressor of
Statement
Reference

sqhE20.E21 is a non-suppressor of mesoderm phenotype of stumps09904b

sqhE20.E21 is a non-suppressor of wing hair phenotype of dsh1

Additional Comments
Genetic Interactions
Statement
Reference

Shorter egg length in sqhE20.E21/+ is enhanced in Pkn1T/Pkn1T or Pkn2T/Pkn2T germline clones.

Introducing one copy of sqhE20.E21 into aurST, aurIM, or aurWK mutants significantly enhances aur mutant lethality.

A rok2/+ heterozygous background suppresses the enhanced lethality of aurIM females bearing one copy of sqhE20.E21, and ameliorates the enhanced rough eye phenotype of these flies.

A rok2/+ heterozygous background suppresses the enhanced lethality of aurWK females bearing one copy of sqhE20.E21, and ameliorates the enhanced rough eye phenotype of these flies.

sqhE20.E21 enhances the neuroepithelial defects of aspE3 larval brains.

Expression of sqhE20.E21 suppresses the short wing and deformed T3 leg phenotypes of DrakKO mutants.

The wing defects seen in flw1/Y ; pucA251.1F3/+ flies are suppressed to wild type by sqhE20.E21.

The epithelial polarity defects and genomic polyploidy defects seen in lkb1X5 larvae are suppressed by sqhE20.E21.

The cuticle defects seen in mutant embryos derived from SNF1AD1 homozygous female germline clones are partially suppressed by sqhE20.E21; the fraction of embryos that form cuticle is increased from approximately 10% to approximately 30%.

The fraction of SNF1AD1 mitotic larval brain neuroblasts that show extreme polyploidy is decreased if the larvae are also mutant for sqhE20.E21.

Expression of one copy of the sqhE20.E21 transgene partially restores axial expansion in Cp1902 germ-line clone embryos.

A single copy of the sqhE20.E21 transgene is able to considerably suppress the stidsRNA.Sym.Scer\UAS (under the regulation of Scer\GAL4ey.PB) rough eye phenotype.

The amount of MbsT541 photoreceptor clones that are mislocalized to the basal region of the eye disc is increased when these discs carry one copy of sqhE20.E21 (a transgene that contains two phosphomimetic mutations).

The flw1 mutation is lethal in combination with the phosphomimetic sqhE20.E21 mutation. The amount of blistering and crumpling of wings in flies expressing MYPT-75DF117A.Scer\UAS under the control of Scer\GAL4Bx-MS1096 is enhanced by sqhE20.E21.

The small, rough eye phenotype of Rac1GMR.PN flies is enhanced by heterozygosity for sqhE20.E21.

4% of rok2/Y ; sqhE20.E21/+ flies survive to adulthood. The multiple wing hair phenotype caused by rok2 is almost completely suppressed by sqhE20.E21. The dsh1 multiple hair phenotype but not the hair misorientation phenotype is suppressed by sqhE20.E21.

Xenogenetic Interactions
Statement
Reference

The midline crossover phenotype caused expression of Ggal\MLCKct.Scer\UAS under the control of Scer\GAL4ftz.ng is enhanced by sqhE20.E21.

Complementation and Rescue Data
Partially rescues
Comments

Expressing one copy of the sqhE20.E21 transgene restores axial expansion in sqh1 germline clones and substantially restores both oogenesis and subsequent axial expansion in sqhAX3 germline clones.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (26)