Allele Dmel\shg^{Ubi-p63E.T:Avic\GFP-rs}

General Information
Symbol	Dmel\shg^{Ubi-p63E.T:Avic\GFP-rs}	Species	D. melanogaster
Name		FlyBase ID	FBal0122908
Feature type	allele	Associated gene	Dmel\shg

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Oda and Tsukita, 2001) in vitro construct - regulatory fusion (Oda and Tsukita, 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Ubi-p63E promoter sequences drive expression of shg tagged at the C-terminal end with T:Avic\GFP^rs. (Oda and Tsukita, 2001)
Carried in construct	P{Ubi-p63E-shg.GFP} (Wood et al., 2006, Schock and Perrimon, 2003, Simoes et al., 2006, Schock and Perrimon, 2002, Ma et al., 2003, Bai and Montell, 2002, Reed et al., 2004, Oda and Tsukita, 2001, McGill et al., 2009, Pastor-Pareja et al., 2004, Stramer et al., 2005, Mirkovic and Mlodzik, 2006, Ninov et al., 2007, Becam et al., 2005, Rodriguez-Diaz et al., 2008, Bischoff and Cseresnyés, 2009, Dyer et al., 2007, Mavrakis et al., 2009, Siekhaus et al., 2010, Sekyrova et al., 2010, Goldbach et al., 2010, Ninov et al., 2010, Martin et al., 2009, He et al., 2010, Abreu-Blanco et al., 2011, Mateus et al., 2011, Olguín et al., 2011, Simões et al., 2010, Olguín et al., 2011, Bosveld et al., 2012, Bosveld et al., 2012, Fichelson et al., 2012, Pereira et al., 2006, Ishihara and Sugimura, 2012, Huang et al., 2011, Mohseni et al., 2009, Haruta et al., 2010)
Tagged with	T:Avic\GFP^rs (Oda and Tsukita, 2001, Schock and Perrimon, 2002, Bai and Montell, 2002, Ma et al., 2003, Schock and Perrimon, 2003, Reed et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	wild-type (Oda and Tsukita, 2001)
Phenotype Manifest In
	egg (Oda and Tsukita, 2001) histoblast \| somatic clone (Ninov et al., 2010) histoblast nest \| somatic clone (Ninov et al., 2010)
Detailed Description
	Statement Reference shg[Ubi-p63E.T:Avic\GFP-rs] clones generated in the histoblasts are largely excluded from the expanding front of the histoblast nest, and are observed remaining in the center of the nest. (Ninov et al., 2010) Expression of shg^{Ubi-p63E.T:Avic\GFP-rs} results in flies laying about 50% unfertilised eggs in some transgenic lines. (Oda and Tsukita, 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference shg^{Ubi-p63E.T:Avic\GFP-rs} is a suppressor \| partially of cytokinesis defective \| male phenotype of Scer\GAL4^{bam.T:Hsim\VP16}, scra^GD9720 (Goldbach et al., 2010)
Phenotype Manifest In
Suppressor of
Statement Reference shg^{Ubi-p63E.T:Avic\GFP-rs} is a suppressor \| partially of spermatid phenotype of Scer\GAL4^{bam.T:Hsim\VP16}, scra^GD9720 (Goldbach et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference shg[Ubi-p63E.T:Avic\GFP-rs] does not significantly change the impaired epidermal cell elongation phenotype seen during dorsal closure stages in embryos expressing Rab11[S25N.Scer\UAS] under the control of Scer\GAL4[en-e16E]. (Mateus et al., 2011) The cytokinesis defects that are seen in the spermatids of males expressing scra[GD9720] under the control of Scer\GAL4[bam.T:Hsim\VP16] are significantly suppressed by co-expression of shg[Ubi-p63E.T:Avic\GFP-rs] such that 39.1% of spermatids are mononucleate, 19.3% are binucleate and 41.6% have four nuclei. (Goldbach et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	shg^{Ubi-p63E.T:Avic\GFP-rs} rescues Df(2R)E2/shg² (Oda and Tsukita, 2001) shg^{Ubi-p63E.T:Avic\GFP-rs} rescues Df(2R)E2/shg^E17B (Oda and Tsukita, 2001) shg^{Ubi-p63E.T:Avic\GFP-rs} rescues Df(2R)E2/shg^g317 (Oda and Tsukita, 2001) shg^{Ubi-p63E.T:Avic\GFP-rs} rescues Df(2R)E2/shg^R69 (Oda and Tsukita, 2001) shg^{Ubi-p63E.T:Avic\GFP-rs} rescues shg^R69 (Haruta et al., 2010)
Comments	Expression of shg[Ubi-p63E.T:Avic\GFP-rs] rescues the lethality of shg[R69]. The head, ventral epidermis and tracheal defects of shg[R69] are almost completely rescued by shg[Ubi-p63E.T:Avic\GFP-rs]. When shg[Ubi-p63E.T:Avic\GFP-rs] is present, shg[R69] mutant cell clones are able to grow in size in the epithelia. These epithelia developed normally into adult tissues. When shg[Ubi-p63E.T:Avic\GFP-rs] is present in the background, egg chambers containing shg[R69] mutant germ-line clones show normal development. Cellularization occurs normally in embryos mutant for both maternal and zygotic shg[R69] that carry shg[Ubi-p63E.T:Avic\GFP-rs]. Similarly, in the presence of shg[Ubi-p63E.T:Avic\GFP-rs], posterior invagination occurs normally, and the ectoderm initiates extension and maintains epithelial integrity in embryos mutant for both maternal and zygotic shg[R69]. (Haruta et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	shg^{Ubi-p63E.T:Avic\GFP-rs} (Bischoff and Cseresnyés, 2009)
Name Synonym
Secondary FlyBase IDs

References ( 37 )

Generate a list of	All references relating to this allele ( 37 )

List References by type	Research paper ( 35 ) Supplementary material ( 2 )
Recent research papers ( 7 )
	Bosveld et al., 2012, Science 336(6082): 724--727 Mechanical control of morphogenesis by Fat/Dachsous/Four-jointed planar cell polarity pathway. [FBrf0218281] Fichelson et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(20): 7893--7898 Orthodenticle and Kruppel homolog 1 regulate Drosophila photoreceptor maturation. [FBrf0218313] Ishihara and Sugimura, 2012, J. Theor. Biol. 313: 201--211 Bayesian inference of force dynamics during morphogenesis. [FBrf0219571] Abreu-Blanco et al., 2011, J. Cell Biol. 193(3): 455--464 Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling. [FBrf0213583] Huang et al., 2011, J. Cell Sci. 124(23): 4001--4013 Differential regulation of adherens junction dynamics during apical-basal polarization. [FBrf0217033] Mateus et al., 2011, PLoS ONE 6(4): e18729 Endocytic and Recycling Endosomes Modulate Cell Shape Changes and Tissue Behaviour during Morphogenesis in Drosophila. [FBrf0213562] Olguín et al., 2011, Curr. Biol. 21(3): 236--242 Intertissue mechanical stress affects frizzled-mediated planar cell polarity in the Drosophila notum epidermis. [FBrf0212947] Show all research papers ...

Allele Dmel\shgUbi-p63E.T:Avic\GFP-rs

Allele Dmel\shg^{Ubi-p63E.T:Avic\GFP-rs}