Allele Dmel\rok^CAT.Scer\UAS

General Information
Symbol	Dmel\rok^CAT.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0122916
Feature type	allele	Associated gene	Dmel\rok

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Winter et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of the catalytic domain (amino acids 1 to 454) of rok. (Winter et al., 2001)
Carried in construct	P{UAS-rok.CAT} (Yan et al., 2009, Billuart et al., 2001, Warner and Longmore, 2009, Lee and Treisman, 2004, Winter et al., 2001, Echard and O'Farrell, 2003, Ninov et al., 2007, Chung et al., 2007, Neisch et al., 2010, Sun et al., 2011, Xu et al., 2008, Corrigall et al., 2007, Okumura et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	cell polarity defective \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C} (Sun et al., 2011) lethal \| pupal stage, with Scer\GAL4^da.G32 (Lee and Treisman, 2004) neuroanatomy defective, with Scer\GAL4^ey-OK107 (Billuart et al., 2001)
Phenotype Manifest In
	embryonic/larval epidermis, with Scer\GAL4^NP0056 (Ninov et al., 2007) mushroom body vertical lobe, with Scer\GAL4^ey-OK107 (Billuart et al., 2001) oocyte \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C} (Sun et al., 2011)
Detailed Description
	Statement Reference Expression of rok[CAT.Scer\UAS] in follicle cells under the control of Scer\GAL4[Scer\FRT.Act5C] produces oocyte polarity defects. (Sun et al., 2011) Expression of rok[CAT.Scer\UAS] under the control of Scer\GAL4[fkh.PH] partially suppresses the salivary gland invagination defects of Rho1[k02107b] embryos. (Xu et al., 2008) Expression of rok^CAT.Scer\UAS in the larval epidermal cells, under the control of Scer\GAL4^NP0056, accelerates the delamination of these cells, leading to the extrusion of more cells than in wild-type larvae. (Ninov et al., 2007) Expression of rok^CAT.Scer\UAS under the control of Scer\GAL4^OK107 results in truncated dorsal lobes in the mushroom body, due to axon retraction. (Billuart et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^da.G32, rok^CAT.Scer\UAS has lethal \| pupal stage phenotype, suppressible by Mbs^{N300.Scer\UAS}, Scer\GAL4^da.G32 (Lee and Treisman, 2004)
Suppressor of
Statement Reference rok^CAT.Scer\UAS, Scer\GAL4^da.G32 is a suppressor of lethal \| pupal stage phenotype of Mbs^{N300.Scer\UAS}, Scer\GAL4^da.G32 (Lee and Treisman, 2004) rok^CAT.Scer\UAS/Scer\GAL4^hs.PB is a suppressor \| partially of visible phenotype of Scer\GAL4^hs.PB, tow^Scer\UAS.cCa (Chung et al., 2007) Scer\GAL4^Bx-MS1096/rok^CAT.Scer\UAS is a suppressor of cytokinesis defective phenotype of Scer\GAL4^Bx-MS1096, pbl^{ΔDH497-549.Scer\UAS} (Echard and O'Farrell, 2003) Scer\GAL4^ptc-559.1/rok^CAT.Scer\UAS is a suppressor \| partially of visible phenotype of mwh⁶/mwh¹ (Yan et al., 2009)
Phenotype Manifest In
NOT Enhancer of
Statement Reference Scer\GAL4^tub.PU/rok^CAT.Scer\UAS is a non-enhancer of eye disc \| somatic clone phenotype of Mbs^T541 (Lee and Treisman, 2004)
Suppressor of
Statement Reference rok^CAT.Scer\UAS/Scer\GAL4^hs.PB is a suppressor \| partially of wing hair \| supernumerary phenotype of Scer\GAL4^hs.PB, tow^Scer\UAS.cCa (Chung et al., 2007) Scer\GAL4^Bx-MS1096/rok^CAT.Scer\UAS is a suppressor of wing hair \| supernumerary phenotype of Scer\GAL4^Bx-MS1096, pbl^{ΔDH497-549.Scer\UAS} (Echard and O'Farrell, 2003) Scer\GAL4^fkh.PH/rok^CAT.Scer\UAS is a suppressor \| partially of presumptive embryonic salivary gland phenotype of Rho1^k02107b (Xu et al., 2008) Scer\GAL4^ptc-559.1/rok^CAT.Scer\UAS is a suppressor \| partially of wing hair \| supernumerary phenotype of mwh⁶/mwh¹ (Yan et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Expression of rok[CAT.Scer\UAS] under the control of Scer\GAL4[ptc-559.1] partially suppresses the mwh[1]/mwh[6] multiple wing hair phenotype. (Yan et al., 2009) The multiple wing hair phenotype caused by expression of tow^Scer\UAS.cCa under the control of Scer\GAL4^hs.PB using heat shock at 24 hours after puparium formation is partially suppressed by co-expression of rok^CAT.Scer\UAS. (Chung et al., 2007) Overexpression of both rok^CAT.Scer\UAS and Mbs^{N300.Scer\UAS}, under the control of Scer\GAL4^da.G32, allow ~30% of flies to survive to adulthood, instead of dying as pupae. The photoreceptor localization defect is not significantly enhanced when Mbs^T541 mutant clones express rok^CAT.Scer\UAS under the control of Scer\GAL4^tub. (Lee and Treisman, 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 3 )
Bloomington	6667 P{UAS-rok.CAT}2.1, y¹ w^* 6668 y¹ w^; P{UAS-rok.CAT}7.1 6669 y¹ w^; P{UAS-rok.CAT}3.1
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	rok^CAT.Scer\UAS
Name Synonym
Secondary FlyBase IDs

References ( 13 )
Research paper	Sun et al., 2011, Development 138(10): 1991--2001 Regulation of somatic myosin activity by Protein Phosphatase 1{beta} controls Drosophila oocyte polarization. [FBrf0213586] Neisch et al., 2010, J. Cell Biol. 189(2): 311--323 Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane. [FBrf0210554] Okumura et al., 2010, Dev. Biol. 344(2): 693--706 Left-right asymmetric morphogenesis of the anterior midgut depends on the activation of a non-muscle myosin II in Drosophila. [FBrf0211383] Warner and Longmore, 2009, J. Cell Biol. 185(6): 1111--1125 Distinct functions for Rho1 in maintaining adherens junctions and apical tension in remodeling epithelia. [FBrf0208183] Yan et al., 2009, Dev. Biol. 333(1): 186--199 Rho1 has multiple functions in Drosophila wing planar polarity. [FBrf0208582] Xu et al., 2008, Dev. Biol. 321(1): 88--100 Rho GTPase controls invagination and cohesive migration of the Drosophila salivary gland through Crumbs and Rho-kinase. [FBrf0206881] Chung et al., 2007, Genetics 176(2): 891--903 The balance between the novel protein target of wingless and the Drosophila Rho-associated kinase pathway regulates planar cell polarity in the Drosophila wing. [FBrf0202398] Corrigall et al., 2007, Dev. Cell 13(5): 730--742 Hedgehog Signaling Is a Principal Inducer of Myosin-II-Driven Cell Ingression in Drosophila Epithelia. [FBrf0202245] Ninov et al., 2007, Development 134(2): 367--379 Extrinsic and intrinsic mechanisms directing epithelial cell sheet replacement during Drosophila metamorphosis. [FBrf0194691] Lee and Treisman, 2004, Mol. Biol. Cell 15(7): 3285--3295 Excessive myosin activity in Mbs mutants causes photoreceptor movement out of the Drosophila eye disc epithelium. [FBrf0180500] Echard and O'Farrell, 2003, Curr. Biol. 13(5): 373--383 The degradation of two mitotic cyclins contributes to the timing of cytokinesis. [FBrf0158812] Billuart et al., 2001, Cell 107(2): 195--207 Regulating axon branch stability. The role of p190 RhoGAP in repressing a retraction signaling pathway. [FBrf0139610] Winter et al., 2001, Cell 105(1): 81--91 Drosophila Rho-associated kinase (Drok) links Frizzled-mediated planar cell polarity signaling to the actin cytoskeleton. [FBrf0135688]

Allele Dmel\rokCAT.Scer\UAS

Allele Dmel\rok^CAT.Scer\UAS