FB2025_01 , released February 20, 2025
Allele: Dmel\par-1N1S.UASp.mGFP6
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General Information
Symbol
Dmel\par-1N1S.UASp.mGFP6
Species
D. melanogaster
Name
FlyBase ID
FBal0122938
Feature type
allele
Associated gene
Dmel\par-1
Associated Insertion(s)
Carried in Construct
P{UASp-par-1.N1S.GFP}
Also Known As
GFP-PAR-1 N1S, Par1-GFP
Key Links
Transgenic product class
wild_type
(Huynh et al., 2001)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Huynh et al., 2001)
Mutagen
in vitro construct
(Huynh et al., 2001)
Progenitor genotype
Carried in construct
P{UASp-par-1.N1S.GFP}
Cytology
Description

UASp regulatory sequences drive expression of the N1S isoform of par-1 tagged at the N-terminal end with mGFP6.

(Huynh et al., 2001)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
par-1
Tagged with
mGFP6
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      oogenesis stage S10 & adult stage | female
      oocyte
      (Sanghavi et al., 2016)
      Additional Information
      Statement
      Reference

      par-1N1S.UASp.mGFP6 is localized on the plasma membrane and enriched at the posterior of the oocyte in control egg chambers .

      (Sanghavi et al., 2016)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  tauopathy
      modeled by Hsap\MAPTV337M.UAS
      (Shulman and Feany, 2003)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The oocyte nucleus is often found mislocalised to the centre of the oocyte in stag 9-10 egg chambers of females expressing two copies of par-1N1S.Scer\UAS.P\T.T:Avic\GFP-m6 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16. Expression of one copy of par-1N1S.Scer\UAS.P\T.T:Avic\GFP-m6 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 has no effect on oocyte nucleus localisation.

      (Tian and Deng, 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The oocyte nucleus is mislocalised to the centre of the oocyte in 43% of egg chambers of females co-expressing par-1N1S.Scer\UAS.P\T.T:Avic\GFP-m6 and l(2)gl3A.Scer\UAS.P\T under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16.

      (Tian and Deng, 2009)
      Xenogenetic Interactions
      Statement
      Reference

      The rough eye phenotype caused by expression of Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed by co-expression of par-1N1S.Scer\UAS.P\T.T:Avic\GFP-m6.

      (Shulman and Feany, 2003)
      Complementation and Rescue Data
      Rescues

      Scer\GAL4CY2/par-1N1S.UASp.mGFP6 rescues par-1k06323/par-1W3

      (Doerflinger et al., 2006)

      Scer\GAL4VP16.nanos.UTR/par-1N1S.UASp.mGFP6 rescues par-1W3

      (Huynh et al., 2001)
      Comments

      Expression of par-1N1S.Scer\UAS.P\T.T:Avic\GFP-m6 in the viable transheterozygote par-1k06323/par-1W3 fully rescues the posterior localisation of osk mRNA in the egg chamber.

      (Doerflinger et al., 2006)

      par-1N1S.Scer\UAS.P\T.T:Avic\GFP-m6 rescues the posterior movement of the centrosomes and the maintenance of oocyte identity when expressed under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in par-1W3 germline clones.

      (Huynh et al., 2001)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      par-1N1S.Scer\UAS.P\T.T:Avic\GFP-m6
      par-1N1S.Scer\UAS.T:Avic\GFP-m6
      par-1N1S.UASp.mGFP6
      Name Synonyms
      Secondary FlyBase IDs
        References (20)