FB2025_01 , released February 20, 2025
Allele: Dmel\Ras85DG12V.UAS
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General Information
Symbol
Dmel\Ras85DG12V.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0123118
Feature type
allele
Associated gene
Dmel\Ras85D
Associated Insertion(s)
Carried in Construct
P{UAS-Ras85D.G12V}
Key Links
Transgenic product class
missense_variant
(Halfar et al., 2001)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Halfar et al., 2001)
Mutagen
in vitro construct
(Halfar et al., 2001)
Progenitor genotype
Carried in construct
P{UAS-Ras85D.G12V}
Cytology
Description

UAS regulatory sequences drive expression of a mutated form of Ras85D (carries the amino acid replacement G12V).

(Halfar et al., 2001)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Ras85D
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cancer
      CEA with RhoGEF2RE.UAS
      (Khoo et al., 2013)
      CEA with RokCAT.UAS
      (Khoo et al., 2013)
      CEA with sqhE20E21.UAS.cCa
      (Khoo et al., 2013)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Eye-antennal disc clones expressing Ras85DG12V.Scer\UAS under the control of Scer\GAL4tub.PU do not exhibit a significant difference in the growth of clonal versus non-clonal tissue.

      (Khoo et al., 2013)

      Primordial germ cell numbers are significantly reduced when Ras85DG12V.Scer\UAS is expressed in the primordial germ cells, under the control of Scer\GAL4C587.

      (Gilboa and Lehmann, 2006)

      Ganglionic branch 1s turn prematurely before reaching the midline in Ras85DG12V.Scer\UAS; Scer\GAL4bs-23.26 embryos.

      (Gallio et al., 2004)

      Clones in the third instar eye disc expressing Ras85DG12V.Scer\UAS under the control of Scer\GAL4Act5C.PP that are located anterior to the morphogenetic furrow are rounded, undergo precocious neuronal development and send out axonal projections.

      (Halfar et al., 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Other
      Phenotype Manifest In
      NOT suppressed by
      Statement
      Reference

      Ras85DG12V.UAS, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by Rac1J11/Rac2Δ/Mtl[+]/MtlΔ/Rac2[+]/Rac1[+]

      (Khoo et al., 2013)

      Ras85DG12V.UAS, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by diaGD9442, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      Ras85DG12V.UAS, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by mengGD7239, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      Ras85DG12V.UAS, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by diaGD9442, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      Ras85DG12V.UAS, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by mengGD7239, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      Ras85DG12V.UAS, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by Rac1N17.UAS, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      Ras85DG12V.UAS, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by Rac1GD17411, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      Ras85DG12V.UAS, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by Rac1N17.UAS, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      Ras85DG12V.UAS, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye photoreceptor cell | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by Rac1GD17411, Scer\GAL4Tub.PU

      (Khoo et al., 2013)

      Ras85DG12V.UAS, RhoGEF2RE.UAS, Scer\GAL4Tub.PU has eye-antennal disc | somatic clone | third instar larval stage phenotype, non-suppressible | somatic clone by Rac1J11/Rac2Δ/Mtl[+]/MtlΔ/Rac2[+]/Rac1[+]

      (Khoo et al., 2013)
      Enhancer of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Ras85DG12V.Scer\UAS enhances the decreased photoreceptor differentiation seen in eye-antennal disc clones expressing RhoGEF2RE.Scer\UAS under the control of Scer\GAL4tub.PU. These clones show overgrowth, similar to those expressing only RhoGEF2RE.Scer\UAS, but also show large undifferentiated clonal masses in the basal regions of the posterior region of the eye disc.

      Co-expression of Rac1N17.Scer\UAS, Rac1GD17411, CG11221GD7239, or diaGD9442 fails to suppress the reduction of differentiation seen in eye-antennal disc clones expressing both RhoGEF2RE.Scer\UAS and Ras85DG12V.Scer\UAS under the control of Scer\GAL4tub.PU, and also fails to suppress the developmental delay shown by larvae containing these clones.

      The presence of Rac2Δ/+, Rac1J11/+ and MtlΔ/+ mutations fails to suppress the reduction of differentiation seen in eye-antennal disc clones expressing both RhoGEF2RE.Scer\UAS and Ras85DG12V.Scer\UAS under the control of Scer\GAL4tub.PU, and also fails to suppress the developmental delay shown by larvae containing these clones.

      Co-expression of Ras85DG12V.Scer\UAS enhances the decreased photoreceptor differentiation seen in eye-antennal disc clones expressing RokCAT.Scer\UAS under the control of Scer\GAL4tub.PU. These clones show large undifferentiated clonal masses. Larvae containing clones expressing both Ras85DG12V.Scer\UAS and RokCAT.Scer\UAS under the control of Scer\GAL4tub.PU exhibit an extended larval period and decrease in pupation rate, as compared with controls.

      Eye-antennal disc clones expressing sqhE20E21.Scer\UAS.cCa and Ras85DG12V.Scer\UAS under the control of Scer\GAL4tub.PU show large masses of undifferentiated tissue in the basal sections of the eye disc, and reduced differentiation of photoreceptor cells, and larvae containing these clones exhibit an extended larval period and reduced pupation rate, as compared to controls.

      (Khoo et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ras85DG12V.Scer\UAS
      Ras85DG12V.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (6)