FlyBase Allele Report: Dmel\NFAT[EP1508]

General Information

Symbol

Dmel\NFAT^EP1508

Species

D. melanogaster

Name

FlyBase ID

FBal0123132

Feature type

allele

Associated gene

Dmel\NFAT

Associated Insertion(s)

P{EP}NFAT^EP1508

Carried in Construct

Also Known As

EP1508, EP(X)1508

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Kraut et al., 2001, Kraut et al., 2001)

Progenitor genotype

Associated Insertion(s)

P{EP}NFAT^EP1508

Cytology

Description

The P{EP} element is inserted within the first intron.

(Kraut et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:13,642,076..13,642,076 [+]

(Freeman et al., 2011, Keyser et al., 2007, Singh et al., 2005, Zhu et al., 2005, Pena-Rangel et al., 2002, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-, Kraut et al., 2001, Kraut et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Bilen and Bonini, 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior fascicle & synapse, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

dorsocentral bristle | increased number, with Scer\GAL4^ap-md544

(Pena-Rangel et al., 2002)

eye, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

eye, with Scer\GAL4^hs.2sev

(Tseng and Hariharan, 2002)

larval intersegmental nerve, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

larval RP2 motor neuron, with Scer\GAL4^{shakB.lethal.4.1}

(Freeman et al., 2011)

neuromuscular junction, with Scer\GAL4^elav-C155

(Freeman et al., 2011)

neuromuscular junction, with Scer\GAL4^RapGAP1-OK6

(Freeman et al., 2011)

neuromuscular junction, with Scer\GAL4^Toll-6-D42

(Freeman et al., 2011)

NMJ bouton, with Scer\GAL4^elav-C155

(Freeman et al., 2011)

scutellar bristle | increased number, with Scer\GAL4^ap-md544

(Pena-Rangel et al., 2002)

synapse, with Scer\GAL4^elav-C155

(Freeman et al., 2011)

synapse, with Scer\GAL4^Toll-6-D42

(Freeman et al., 2011)

wing vein | increased number, with Scer\GAL4^ap-md544

(Pena-Rangel et al., 2002)

Detailed Description

Statement

Reference

Pan-neuronal NFAT overexpression through Scer\GAL4^elav-C155-driven expression of NFAT^EP1508 results in significantly smaller synapses with clusters of terminal synaptic boutons and an overall reduction in the expanse of the pre-synaptic arbor.

Post-synaptic muscle expression of NFAT^EP1508 under the control of Scer\GAL4^Mef2.PR has no observable effect on neuromuscular junction size.

Expression of NFAT^EP1508 under the control of Scer\GAL4^D42 results in neuromuscular junctions with reduced synapse length compared to controls.

Neuronal expression of NFAT^EP1508 (under the control of Scer\GAL4^elav-C155) reduces transmitter release (mean EJC amplitude) while spontaneous mini EJCs are unaffected. The number of active zones are not affected in these mutants.

Overexpression of NFAT^EP1508 in RP2 neurons under the control of Scer\GAL4^{shakB.lethal.4.1} dramatically reduces the excitability of RP2, in that the input resistance is reduced to half of the wild-type. As a consequence of this drastic increase in the leakiness of the cell NFAT neurons depolarize much less, and produce fewer action potentials than controls.

Larve expressing NFAT^EP1508 pan-neuronally (under the control of Scer\GAL4^elav-C155) move significantly slower than control animals and thus cover a smaller distance in an allotted time. Moreover, as compared to a relatively straight trajectory in wild-type animals, NFAT^EP1508-expressing larvae also display a greater degree of turning and 'rolling'.

Expression of NFAT^EP1508, driven by Scer\GAL4^OK6 in an otherwise wild-type background results in a slightly reduced neuromuscular junction size.

(Freeman et al., 2011)

Flies expressing NFAT^EP1508 under the control of Scer\GAL4^ap-md544 have extra veins, dorsocentral and scutellar macrochaetae.

(Pena-Rangel et al., 2002)

Expression of NFAT^EP1508 under the control of Scer\GAL4^ey.PH results in eyes that are strongly reduced in size. Flies expressing NFAT^EP1508 under the control of Scer\GAL4^hs.2sev have mildly rough eyes. Flies expressing NFAT^EP1508 under the control of Scer\GAL4^en-e16E have wild-type wings.

(Tseng and Hariharan, 2002)

Mutant larvae expressing NFAT^EP1508 under the control of Scer\GAL4^elav-C155 have reduced or abnormal synapses. Mutant larvae expressing NFAT^EP1508 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects.

(Kraut et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

NFAT^EP1508 has abnormal neuroanatomy phenotype, suppressible by futsch^N94/futsch[+]

(Freeman et al., 2011)

NOT suppressed by

Statement

Reference

NFAT^EP1508, Scer\GAL4^ey.PH has visible phenotype, non-suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

Enhancer of

Statement

Reference

NFAT^EP1508, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Cdc42^L89.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

Scer\GAL4^pnr-MD237/NFAT^EP1508 is an enhancer of visible phenotype of pnr^MD237

(Pena-Rangel et al., 2002)

NOT Enhancer of

Statement

Reference

NFAT^EP1508, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

NFAT^EP1508, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, btl::Egfr^λ.UAS

(Zhu et al., 2005)

Suppressor of

Statement

Reference

NFAT^EP1508, Scer\GAL4^GMR.PU is a suppressor of visible phenotype of Hsap\HSPA1L^K71E.UAS, Scer\GAL4^GMR.PU

(Bilen and Bonini, 2007)

NFAT^EP1508, Scer\GAL4^GMR.PU is a suppressor of abnormal neuroanatomy | adult stage phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Bilen and Bonini, 2007)

NFAT^EP1508/Scer\GAL4^ey.PH is a suppressor of visible | dominant phenotype of L²

(Singh et al., 2005)

NFAT^EP1508, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

NFAT^EP1508, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Pvr^λ.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

NOT Suppressor of

Statement

Reference

NFAT^EP1508, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

NFAT^EP1508, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, btl::Egfr^λ.UAS

(Zhu et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

NFAT^EP1508, Scer\GAL4^Toll-6-D42 has synapse phenotype, suppressible by futsch^N94/futsch[+]

(Freeman et al., 2011)

NOT suppressed by

Statement

Reference

NFAT^EP1508, Scer\GAL4^ey.PH has eye phenotype, non-suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

Enhancer of

Statement

Reference

Scer\GAL4^pnr-MD237/NFAT^EP1508 is an enhancer of adult thorax phenotype of pnr^MD237

(Pena-Rangel et al., 2002)

Scer\GAL4^pnr-MD237/NFAT^EP1508 is an enhancer of chaeta phenotype of pnr^MD237

(Pena-Rangel et al., 2002)

NOT Enhancer of

Statement

Reference

NFAT^EP1508, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, btl::Egfr^λ.UAS

(Zhu et al., 2005)

Suppressor of

Statement

Reference

Scer\GAL4^RapGAP1-OK6/NFAT^EP1508 is a suppressor of synapse phenotype of SERCA^Kum170, comt⁶

(Freeman et al., 2011)

NFAT^EP1508, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Hsap\HSPA1L^K71E.UAS, Scer\GAL4^GMR.PU

(Bilen and Bonini, 2007)

NFAT^EP1508, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Bilen and Bonini, 2007)

NFAT^EP1508/Scer\GAL4^ey.PH is a suppressor of eye | ventral phenotype of L²

(Singh et al., 2005)

NFAT^EP1508, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

NFAT^EP1508, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Pvr^λ.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

NOT Suppressor of

Statement

Reference

NFAT^EP1508, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, btl::Egfr^λ.UAS

(Zhu et al., 2005)

Other

Statement

Reference

NFAT^EP1508, Scer\GAL4^pnr-MD237, pnr^MD237/pnr[+] has macrochaeta phenotype

(Pena-Rangel et al., 2002)

NFAT^EP1508, Scer\GAL4^pnr-MD237, pnr^MD237/pnr[+] has microchaeta phenotype

(Pena-Rangel et al., 2002)

NFAT^EP1508, Scer\GAL4^pnr-MD237, pnr^MD237 has macrochaeta phenotype

(Pena-Rangel et al., 2002)

NFAT^EP1508, Scer\GAL4^pnr-MD237, pnr^MD237 has microchaeta phenotype

(Pena-Rangel et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

A futsch^N94 heterozygous background restores normal synaptic length when NFAT^EP1508 is expressed under the control of Scer\GAL4^D42.

Scer\GAL4^OK6-driven motor neuron-limited expression of NFAT^EP1508 completely abolishes increased synapse growth and transmitter release observed in comt⁶ Ca-P60A^Kum170 double mutants.

(Freeman et al., 2011)

pnr^MD237/+ adults expressing NFAT^EP1508 under the control of Scer\GAL4^pnr-MD237 have a slight thoracic cleft, deformed macrochaetae and loss of microchaetae.

(Pena-Rangel et al., 2002)

Co-expression of CycE^Scer\UAS.cLa does not suppress the small eye phenotype caused by expression of NFAT^EP1508 under the control of Scer\GAL4^ey.PH.

(Tseng and Hariharan, 2002)

Xenogenetic Interactions

Statement

Reference

NFAT^EP1508 is a moderate suppressor of the eye degeneration phenotype caused by expression of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PU.

(Bilen and Bonini, 2007)

Complementation and Rescue Data

Rescued by

NFAT^EP1508 is rescued by Scer\GAL4^elav-C155/NFAT^RHD.UAS

(Freeman et al., 2011)

Comments

Co-expression of NFAT^RHD.Scer\UAS pan-neuronally with Scer\GAL4^elav-C155 rescues synapse size in NFAT^EP1508 expression mutants.

(Freeman et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a P{EP} insertion line that modifies the pnr^MD237/+ phenotype when expressed using Scer\GAL4^pnr-MD237.

(Pena-Rangel et al., 2002)

Comments

P{EP}NFAT^EP1508 is in a position to drive expression of NFAT isoform A.

(Freeman et al., 2011)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

NFAT^EP1508

(Bilen and Bonini, 2007)

Name Synonyms

Secondary FlyBase IDs

References (10)

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