Allele Dmel\NFAT^EP1508

General Information
Symbol	Dmel\NFAT^EP1508	Species	D. melanogaster
Name		FlyBase ID	FBal0123132
Feature type	allele	Associated gene	Dmel\NFAT

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Kraut et al., 2001, Kraut et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site X:13,536,109..13,536,109 (Kraut et al., 2001, Singh et al., 2005, Zhu et al., 2005, Pena-Rangel et al., 2002, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-, Kraut et al., 2001, Keyser et al., 2007, Freeman et al., 2011)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference The P{EP} element is inserted within the first intron. (Kraut et al., 2001)
Caused by insertion	P{EP}NFAT^EP1508 (Kraut et al., 2001, Singh et al., 2005, Zhu et al., 2005, Pena-Rangel et al., 2002, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-, Kraut et al., 2001, Keyser et al., 2007, Freeman et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	locomotor rhythm defective, with Scer\GAL4^elav-C155 (Freeman et al., 2011) neuroanatomy defective, with Scer\GAL4^D42 (Freeman et al., 2011) neuroanatomy defective, with Scer\GAL4^Rapgap1-OK6 (Freeman et al., 2011) neuroanatomy defective \| larval stage, with Scer\GAL4^elav-C155 (Kraut et al., 2001) neurophysiology defective, with Scer\GAL4^elav-C155 (Freeman et al., 2011) neurophysiology defective, with Scer\GAL4^{shakB.lethal.4.1} (Freeman et al., 2011) visible, with Scer\GAL4^ap-md544 (Pena-Rangel et al., 2002) visible, with Scer\GAL4^ey.PH (Tseng and Hariharan, 2002) visible, with Scer\GAL4^hs.2sev (Tseng and Hariharan, 2002)
Phenotype Manifest In
	anterior fascicle & synapse, with Scer\GAL4^elav-C155 (Kraut et al., 2001) dorsocentral bristle \| supernumerary, with Scer\GAL4^ap-md544 (Pena-Rangel et al., 2002) eye, with Scer\GAL4^ey.PH (Tseng and Hariharan, 2002) eye, with Scer\GAL4^hs.2sev (Tseng and Hariharan, 2002) intersegmental nerve, with Scer\GAL4^elav-C155 (Kraut et al., 2001) neuromuscular junction, with Scer\GAL4^D42 (Freeman et al., 2011) neuromuscular junction, with Scer\GAL4^elav-C155 (Freeman et al., 2011) neuromuscular junction, with Scer\GAL4^Rapgap1-OK6 (Freeman et al., 2011) NMJ bouton, with Scer\GAL4^elav-C155 (Freeman et al., 2011) RP2 motor neuron, with Scer\GAL4^{shakB.lethal.4.1} (Freeman et al., 2011) scutellar bristle \| supernumerary, with Scer\GAL4^ap-md544 (Pena-Rangel et al., 2002) synapse, with Scer\GAL4^D42 (Freeman et al., 2011) synapse, with Scer\GAL4^elav-C155 (Freeman et al., 2011) wing vein \| supernumerary, with Scer\GAL4^ap-md544 (Pena-Rangel et al., 2002)
Detailed Description
	Statement Reference Pan-neuronal NFAT overexpression through Scer\GAL4[elav-C155]-driven expression of NFAT[EP1508] results in significantly smaller synapses with clusters of terminal synaptic boutons and an overall reduction in the expanse of the pre-synaptic arbor. Post-synaptic muscle expression of NFAT[EP1508] under the control of Scer\GAL4[Mef2.PR] has no observable effect on neuromuscular junction size. Expression of NFAT[EP1508] under the control of Scer\GAL4[D42] results in neuromuscular junctions with reduced synapse length compared to controls. Neuronal expression of NFAT[EP1508] (under the control of Scer\GAL4[elav-C155]) reduces transmitter release (mean EJC amplitude) while spontaneous mini EJCs are unaffected. The number of active zones are not affected in these mutants. Overexpression of NFAT[EP1508] in RP2 neurons under the control of Scer\GAL4[shakB.lethal.4.1] dramatically reduces the excitability of RP2, in that the input resistance is reduced to half of the wild-type. As a consequence of this drastic increase in the leakiness of the cell NFAT neurons depolarize much less, and produce fewer action potentials than controls. Larve expressing NFAT[EP1508] pan-neuronally (under the control of Scer\GAL4[elav-C155]) move significantly slower than control animals and thus cover a smaller distance in an allotted time. Moreover, as compared to a relatively straight trajectory in wild-type animals, NFAT[EP1508]-expressing larvae also display a greater degree of turning and 'rolling'. Expression of NFAT[EP1508], driven by Scer\GAL4[OK6] in an otherwise wild-type background results in a slightly reduced neuromuscular junction size. (Freeman et al., 2011) Flies expressing NFAT^EP1508 under the control of Scer\GAL4^ap-md544 have extra veins, dorsocentral and scutellar macrochaetae. (Pena-Rangel et al., 2002) Expression of NFAT^EP1508 under the control of Scer\GAL4^ey.PH results in eyes that are strongly reduced in size. Flies expressing NFAT^EP1508 under the control of Scer\GAL4^hs.2sev have mildly rough eyes. Flies expressing NFAT^EP1508 under the control of Scer\GAL4^en-e16E have wild-type wings. (Tseng and Hariharan, 2002) Mutant larvae expressing NFAT^EP1508 under the control of Scer\GAL4^elav-C155 have reduced or abnormal synapses. Mutant larvae expressing NFAT^EP1508 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects. (Kraut et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference NFAT^EP1508 has neuroanatomy defective phenotype, suppressible by futsch^N94/futsch[+] (Freeman et al., 2011)
NOT suppressed by
Statement Reference NFAT^EP1508, Scer\GAL4^ey.PH has visible phenotype, non-suppressible by CycE^Scer\UAS.cLa, Scer\GAL4^ey.PH (Tseng and Hariharan, 2002)
Enhancer of
Statement Reference NFAT^EP1508, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Cdc42^L89.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Zhu et al., 2005) Scer\GAL4^pnr-MD237/NFAT^EP1508 is an enhancer of visible phenotype of pnr^MD237 (Pena-Rangel et al., 2002)
NOT Enhancer of
Statement Reference NFAT^EP1508, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Rac1^N17.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Zhu et al., 2005) NFAT^EP1508, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, btl::Egfr^{Scer\UAS.T:λ\cI-DD} (Zhu et al., 2005)
Suppressor of
Statement Reference NFAT^EP1508, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Pvr^{Scer\UAS.T:λ\cI-DD}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Zhu et al., 2005) NFAT^EP1508, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, btl^{Scer\UAS.T:λ\cI-DD}, stumps^{Scer\UAS.T:Zzzz\FLAG} (Zhu et al., 2005) NFAT^EP1508, Scer\GAL4^GMR.PU is a suppressor \| partially of neuroanatomy defective \| adult stage phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) NFAT^EP1508, Scer\GAL4^GMR.PU is a suppressor of visible phenotype of Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) NFAT^EP1508/Scer\GAL4^ey.PH is a suppressor \| partially of visible \| dominant phenotype of L² (Singh et al., 2005)
NOT Suppressor of
Statement Reference NFAT^EP1508, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Rac1^N17.Scer\UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Zhu et al., 2005) NFAT^EP1508, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, btl::Egfr^{Scer\UAS.T:λ\cI-DD} (Zhu et al., 2005)
Phenotype Manifest In
Suppressed by
Statement Reference NFAT^EP1508, Scer\GAL4^D42 has synapse phenotype, suppressible by futsch^N94/futsch[+] (Freeman et al., 2011)
NOT suppressed by
Statement Reference NFAT^EP1508, Scer\GAL4^ey.PH has eye phenotype, non-suppressible by CycE^Scer\UAS.cLa, Scer\GAL4^ey.PH (Tseng and Hariharan, 2002)
Enhancer of
Statement Reference Scer\GAL4^pnr-MD237/NFAT^EP1508 is an enhancer of adult thorax phenotype of pnr^MD237 (Pena-Rangel et al., 2002) Scer\GAL4^pnr-MD237/NFAT^EP1508 is an enhancer of chaeta phenotype of pnr^MD237 (Pena-Rangel et al., 2002)
NOT Enhancer of
Statement Reference NFAT^EP1508, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, btl::Egfr^{Scer\UAS.T:λ\cI-DD} (Zhu et al., 2005)
Suppressor of
Statement Reference NFAT^EP1508, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Pvr^{Scer\UAS.T:λ\cI-DD}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Zhu et al., 2005) NFAT^EP1508, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, btl^{Scer\UAS.T:λ\cI-DD}, stumps^{Scer\UAS.T:Zzzz\FLAG} (Zhu et al., 2005) NFAT^EP1508, Scer\GAL4^GMR.PU is a suppressor \| partially of eye phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) NFAT^EP1508, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Hsap\HSPA1L^{K71E.Scer\UAS}, Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) NFAT^EP1508/Scer\GAL4^ey.PH is a suppressor \| partially of eye \| ventral phenotype of L² (Singh et al., 2005) Scer\GAL4^Rapgap1-OK6/NFAT^EP1508 is a suppressor of synapse phenotype of Ca-P60A^Kum170, comt⁶ (Freeman et al., 2011)
NOT Suppressor of
Statement Reference NFAT^EP1508, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, btl::Egfr^{Scer\UAS.T:λ\cI-DD} (Zhu et al., 2005)
Other
Statement Reference NFAT^EP1508, Scer\GAL4^pnr-MD237, pnr^MD237/pnr[+] has macrochaeta phenotype (Pena-Rangel et al., 2002) NFAT^EP1508, Scer\GAL4^pnr-MD237, pnr^MD237/pnr[+] has microchaeta phenotype (Pena-Rangel et al., 2002) NFAT^EP1508, Scer\GAL4^pnr-MD237, pnr^MD237 has macrochaeta phenotype (Pena-Rangel et al., 2002) NFAT^EP1508, Scer\GAL4^pnr-MD237, pnr^MD237 has microchaeta phenotype (Pena-Rangel et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference A futsch[N94] heterozygous background restores normal synaptic length when NFAT[EP1508] is expressed under the control of Scer\GAL4[D42]. Scer\GAL4[OK6]-driven motor neuron-limited expression of NFAT[EP1508] completely abolishes increased synapse growth and transmitter release observed in comt[6] Ca-P60A[Kum170] double mutants. (Freeman et al., 2011) pnr^MD237/+ adults expressing NFAT^EP1508 under the control of Scer\GAL4^pnr-MD237 have a slight thoracic cleft, deformed macrochaetae and loss of microchaetae. (Pena-Rangel et al., 2002) Co-expression of CycE^Scer\UAS.cLa does not suppress the small eye phenotype caused by expression of NFAT^EP1508 under the control of Scer\GAL4^ey.PH. (Tseng and Hariharan, 2002)
Xenogenetic Interactions
Statement Reference NFAT[EP1508] is a moderate suppressor of the eye degeneration phenotype caused by expression of Hsap\ATXN3[tr.Q78.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[GMR.PU]. (Bilen and Bonini, 2007)
Complementation & Rescue Data
Rescued by	NFAT^EP1508 is rescued by Scer\GAL4^elav-C155/NFAT^RHD.Scer\UAS (Freeman et al., 2011)
Comments	Co-expression of NFAT[RHD.Scer\UAS] pan-neuronally with Scer\GAL4[elav-C155] rescues synapse size in NFAT[EP1508] expression mutants. (Freeman et al., 2011)
Stocks ( 0 )

Notes on Origin
Discoverer
	Selected as: a P{EP} insertion line that modifies the pnr^MD237/+ phenotype when expressed using Scer\GAL4^pnr-MD237. (Pena-Rangel et al., 2002)
Comments
	P{EP}NFAT[EP1508] is in a position to drive expression of NFAT isoform A. (Freeman et al., 2011)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	NFAT^EP1508 (Bilen and Bonini, 2007)
Name Synonym
Secondary FlyBase IDs

References ( 10 )
Research paper	Freeman et al., 2011, Mol. Cell. Neurosci. 46(2): 535--547 NFAT regulates pre-synaptic development and activity-dependent plasticity in Drosophila. [FBrf0212894] Bilen and Bonini, 2007, PLoS Genet. 3(10): e177 Genome-wide screen for modifiers of ataxin-3 neurodegeneration in Drosophila. [FBrf0202746] Keyser et al., 2007, Insect Biochem. Mol. Biol. 37(4): 356--362 The Drosophila NFAT homolog is involved in salt stress tolerance. [FBrf0200725] Singh et al., 2005, Genetics 171(1): 169--183 Genetic interaction of Lobe with its modifiers in dorsoventral patterning and growth of the Drosophila eye. [FBrf0187660] Zhu et al., 2005, Genetics 170(2): 767--777 A screen for genes that influence fibroblast growth factor signal transduction in Drosophila. [FBrf0187664] Pena-Rangel et al., 2002, Genetics 160(3): 1035--1050 A misexpression study examining dorsal thorax formation in Drosophila melanogaster. [FBrf0147055] Tseng and Hariharan, 2002, Genetics 162(1): 229--243 An overexpression screen in Drosophila for genes that restrict growth or cell-cycle progression in the developing eye. [FBrf0152035] Kraut et al., 2001, Curr. Biol. 11(6): 417--430 A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135708]
Supplementary material	Kraut et al., 2001, Curr. Biol. 11(6): A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135968]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]

Allele Dmel\NFATEP1508

Allele Dmel\NFAT^EP1508