Open Close
General Information
Symbol
Dmel\NFATEP1508
Species
D. melanogaster
Name
FlyBase ID
FBal0123132
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
EP1508, EP(X)1508
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The P{EP} element is inserted within the first intron.

Insertion components
P{EP}NFATEP1508
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Pan-neuronal NFAT overexpression through Scer\GAL4elav-C155-driven expression of NFATEP1508 results in significantly smaller synapses with clusters of terminal synaptic boutons and an overall reduction in the expanse of the pre-synaptic arbor.

Post-synaptic muscle expression of NFATEP1508 under the control of Scer\GAL4Mef2.PR has no observable effect on neuromuscular junction size.

Expression of NFATEP1508 under the control of Scer\GAL4D42 results in neuromuscular junctions with reduced synapse length compared to controls.

Neuronal expression of NFATEP1508 (under the control of Scer\GAL4elav-C155) reduces transmitter release (mean EJC amplitude) while spontaneous mini EJCs are unaffected. The number of active zones are not affected in these mutants.

Overexpression of NFATEP1508 in RP2 neurons under the control of Scer\GAL4shakB.lethal.4.1 dramatically reduces the excitability of RP2, in that the input resistance is reduced to half of the wild-type. As a consequence of this drastic increase in the leakiness of the cell NFAT neurons depolarize much less, and produce fewer action potentials than controls.

Larve expressing NFATEP1508 pan-neuronally (under the control of Scer\GAL4elav-C155) move significantly slower than control animals and thus cover a smaller distance in an allotted time. Moreover, as compared to a relatively straight trajectory in wild-type animals, NFATEP1508-expressing larvae also display a greater degree of turning and 'rolling'.

Expression of NFATEP1508, driven by Scer\GAL4OK6 in an otherwise wild-type background results in a slightly reduced neuromuscular junction size.

Flies expressing NFATEP1508 under the control of Scer\GAL4ap-md544 have extra veins, dorsocentral and scutellar macrochaetae.

Expression of NFATEP1508 under the control of Scer\GAL4ey.PH results in eyes that are strongly reduced in size. Flies expressing NFATEP1508 under the control of Scer\GAL4hs.2sev have mildly rough eyes. Flies expressing NFATEP1508 under the control of Scer\GAL4en-e16E have wild-type wings.

Mutant larvae expressing NFATEP1508 under the control of Scer\GAL4elav-C155 have reduced or abnormal synapses. Mutant larvae expressing NFATEP1508 under the control of Scer\GAL4elav-C155 have ISN neuron pathfinding defects.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

NFATEP1508 has neuroanatomy defective phenotype, suppressible by futschN94/futsch[+]

NOT suppressed by
Statement
Reference
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Suppressed by
Statement
Reference

NFATEP1508, Scer\GAL4Toll-6-D42 has synapse phenotype, suppressible by futschN94/futsch[+]

NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

A futschN94 heterozygous background restores normal synaptic length when NFATEP1508 is expressed under the control of Scer\GAL4D42.

Scer\GAL4OK6-driven motor neuron-limited expression of NFATEP1508 completely abolishes increased synapse growth and transmitter release observed in comt6 Ca-P60AKum170 double mutants.

pnrMD237/+ adults expressing NFATEP1508 under the control of Scer\GAL4pnr-MD237 have a slight thoracic cleft, deformed macrochaetae and loss of microchaetae.

Co-expression of CycEScer\UAS.cLa does not suppress the small eye phenotype caused by expression of NFATEP1508 under the control of Scer\GAL4ey.PH.

Xenogenetic Interactions
Statement
Reference

NFATEP1508 is a moderate suppressor of the eye degeneration phenotype caused by expression of Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU.

Complementation and Rescue Data
Comments

Co-expression of NFATRHD.Scer\UAS pan-neuronally with Scer\GAL4elav-C155 rescues synapse size in NFATEP1508 expression mutants.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Selected as: a P{EP} insertion line that modifies the pnrMD237/+ phenotype when expressed using Scer\GAL4pnr-MD237.

Comments
Comments

P{EP}NFATEP1508 is in a position to drive expression of NFAT isoform A.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (10)