Pan-neuronal NFAT overexpression through Scer\GAL4elav-C155-driven expression of NFATEP1508 results in significantly smaller synapses with clusters of terminal synaptic boutons and an overall reduction in the expanse of the pre-synaptic arbor.
Post-synaptic muscle expression of NFATEP1508 under the control of Scer\GAL4Mef2.PR has no observable effect on neuromuscular junction size.
Expression of NFATEP1508 under the control of Scer\GAL4D42 results in neuromuscular junctions with reduced synapse length compared to controls.
Neuronal expression of NFATEP1508 (under the control of Scer\GAL4elav-C155) reduces transmitter release (mean EJC amplitude) while spontaneous mini EJCs are unaffected. The number of active zones are not affected in these mutants.
Overexpression of NFATEP1508 in RP2 neurons under the control of Scer\GAL4shakB.lethal.4.1 dramatically reduces the excitability of RP2, in that the input resistance is reduced to half of the wild-type. As a consequence of this drastic increase in the leakiness of the cell NFAT neurons depolarize much less, and produce fewer action potentials than controls.
Larve expressing NFATEP1508 pan-neuronally (under the control of Scer\GAL4elav-C155) move significantly slower than control animals and thus cover a smaller distance in an allotted time. Moreover, as compared to a relatively straight trajectory in wild-type animals, NFATEP1508-expressing larvae also display a greater degree of turning and 'rolling'.
Expression of NFATEP1508, driven by Scer\GAL4OK6 in an otherwise wild-type background results in a slightly reduced neuromuscular junction size.