Allele Dmel\JIL-1^EP3657

General Information
Symbol	Dmel\JIL-1^EP3657	Species	D. melanogaster
Name		FlyBase ID	FBal0123148
Feature type	allele	Associated gene	Dmel\JIL-1
Also Known As	EP3, JIL-1^EP(3)3657

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Kraut et al., 2001, Kraut et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3L:11,085,410..11,085,410 (Kraut et al., 2001, Gene Disruption Project members, 2001-, Wang et al., 2001, Kraut et al., 2001)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference The P{EP} element is inserted 269 nucleotides 5' to the predicted ATG start codon, within the 5' UTR. (Kraut et al., 2001) P{EP} insertion in the first exon approximately 700bp upstream of the putative start codon. (Wang et al., 2001)
Caused by insertion	P{EP}JIL-1^EP3657 (Kraut et al., 2001, Gene Disruption Project members, 2001-, Wang et al., 2001, Kraut et al., 2001)
Cytology
Phenotypic Data
Phenotypic Class
	fertile, with Scer\GAL4[-]: (Wang et al., 2001) lethal, with Scer\GAL4[-]: (Zhang et al., 2003) lethal \| embryonic stage \| maternal effect (with JIL-1^z2), with Scer\GAL4[-]: (Zhang et al., 2003) lethal \| partially \| recessive, with Scer\GAL4[-]: (Zhang et al., 2003) visible \| homeotic, with Scer\GAL4[-]: (Zhang et al., 2003) visible \| homeotic (with JIL-1^z2), with Scer\GAL4[-]: (Zhang et al., 2003)
Phenotype Manifest In
	abdominal sternite 5 \| ectopic \| male, with Scer\GAL4[-]: (Zhang et al., 2003) abdominal sternite 5 \| ectopic \| male (with JIL-1^z2), with Scer\GAL4[-]: (Zhang et al., 2003) abdominal sternite 6 \| male, with Scer\GAL4[-]: (Zhang et al., 2003) abdominal sternite 6 \| male (with JIL-1^z2), with Scer\GAL4[-]: (Zhang et al., 2003) chromatin, with Scer\GAL4[-]: (Wang et al., 2001) intersegmental nerve, with Scer\GAL4^elav-C155 (Kraut et al., 2001) nucleus, with Scer\GAL4[-]: (Wang et al., 2001) ovary, with Scer\GAL4[-]: (Zhang et al., 2003) polytene chromosome, with Scer\GAL4[-]: (Wang et al., 2001) spindle, with Scer\GAL4[-]: (Wang et al., 2001)
Detailed Description
	Statement Reference The hatch rate of homozygous embryos produced by homozygous parents is as low as 4-7%. (Zhang et al., 2003) About 80% of homozygous animals derived from heterozygous parents eclose, although subsequent generations show further decreased viability, probably due to decreased levels of maternal JIL-1 product. Only 8% of embryos derived from JIL-1^z2/JIL-1^EP3657 females mated to wild-type males hatch, of those larvae that hatch, 58% survive to pupation, and of those that survive to pupation 95% eclose. 94% of embryos derived from wild-type females mated to JIL-1^z2/JIL-1^EP3657 males hatch, and of those larvae that hatch, 95% survive to pupation. 87% of embryos derived from JIL-1^z2/+ females mated to JIL-1^z2/JIL-1^EP3657 males hatch, suggesting that some embryos with the JIL-1^z2/JIL-1^z2 or JIL-1^z2/JIL-1^EP3657 genotype can survive embryogenesis if provided with normal maternal JIL-1 product. No JIL-1^z2/JIL-1^z2 progeny survive to eclosion and the number of surviving JIL-1^z2/JIL-1^EP3657 adults is about half that of comparable sibling classes. Homozygous ovaries are moderately decreased in size compared to wild-type ovaries. In 88% of homozygous males, the A6 sternite is covered by one or more large bristles, indicating a transformation of A6 to the more anterior A5 segment. 93% of JIL-1^z2/JIL-1^EP3657 males have ventral sternite bristles on the A6 sternite, indicating a transformation of A6 to the more anterior A5 segment. (Zhang et al., 2003) Mutant larvae expressing JIL-1^EP3657 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects. (Kraut et al., 2001) Homozygous larvae (derived from heterozygous females) have an eclosion rate of 81%. The number of males eclosing is 73% that of females. Eclosed adults are fertile and able to produce offspring. The hatch rate of embryos derived from homozygous parents is only 4%. The number of males eclosing is 48% that of females. Embryos derived from homozygous parents show a range of phenotypes from embryos appearing wild type with regularly spaced nuclei to embryos where chromatin structure is completely disintegrated. In intermediate phenotypes, nuclei in various stages of fragmentation are discernible. Centrosomes are often seen to be separated from the nuclear remnants and in other cases aberrant and misaligned tubulin spindles are seen. The polytene chromosome banding pattern is relatively normal in homozygous third instar larvae. Perturbation of the male X chromosome is relatively more severe than that of the autosomes which are only subtly affected. (Wang et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference JIL-1^EP3657, Scer\GAL4[-] has lethal phenotype, suppressible by lola[+]/lola⁰⁰⁶⁴² (Zhang et al., 2003)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference The hatch rate of lola⁰⁰⁶⁴²/+ ; JIL-1^EP3657/JIL-1^EP3657 embryos is 20%. (Zhang et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	JIL-1^EP3657 is rescued by JIL-1^{Hsp83.T:Avic\GFP}/Scer\GAL4[-] (Wang et al., 2001)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	General trend from least to most viable is: JIL-1^z2 < JIL-1^h9 < JIL-1^z60 < JIL-1^EP3657 < JIL-1^z28 ~ JIL-1^Scim. (Zhang et al., 2003)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	EP3 (Zhang et al., 2003, Wang et al., 2001) EP(3)3657 (Wang et al., 2001) JIL-1^EP3657 JIL-1^EP(3)3657 (Zhang et al., 2003, Zhang et al., 2003)
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Zhang et al., 2003, Genetics 165(3): 1341--1354 Genetic and phenotypic analysis of alleles of the Drosophila chromosomal JIL-1 kinase reveals a functional requirement at multiple developmental stages. [FBrf0167636] Zhang et al., 2003, J. Biol. Chem. 278(13): 11696--11704 A developmentally regulated splice variant from the complex lola locus encoding multiple different zinc finger domain proteins interacts with the chromosomal kinase JIL-1. [FBrf0159022] Kraut et al., 2001, Curr. Biol. 11(6): 417--430 A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135708] Wang et al., 2001, Cell 105(4): 433--443 The JIL-1 tandem kinase mediates histone H3 phosphorylation and is required for maintenance of chromatin structure in Drosophila. [FBrf0136815]
Supplementary material	Kraut et al., 2001, Curr. Biol. 11(6): A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135968]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]

Allele Dmel\JIL-1EP3657

Allele Dmel\JIL-1^EP3657