Allele Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}

General Information
Symbol	Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}	Species	H. sapiens
Name		FlyBase ID	FBal0123159
Feature type	allele	Associated gene	Hsap\KCNJ2

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02	Controlled Vocabulary Terms embryonic/larval olfactory receptor neuron projection neuron
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Baines et al., 2001, Hardie et al., 2001) in vitro construct - regulatory fusion (Baines et al., 2001, Hardie et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of Hsap\KCNJ2 tagged with T:Avic\GFP^EGFP (EGFP). (Baines et al., 2001)
Carried in construct	P{UAS-Hsap\KCNJ2.EGFP} (Nitabach et al., 2002, Kueppers et al., 2003, Luan et al., 2006, Nitabach et al., 2006, Malpel et al., 2002, Paradis et al., 2001, Hardie et al., 2002, Baines et al., 2001, Olofsson and Page, 2005, Larsson et al., 2004, Tsai et al., 2004, Hardie et al., 2004, Hardie et al., 2001, Fischler et al., 2007, Gordon and Scott, 2009, Joiner et al., 2006, Hardie et al., 2007, Suster et al., 2003, King et al., 2011, Keene et al., 2011, Yuan et al., 2011, Depetris-Chauvin et al., 2011, Yang et al., 2008, Hergarden et al., 2012, Koon et al., 2011, Dankert et al., 2009, Wang and Anderson, 2010, Peabody et al., 2008, Cognigni et al., 2011, Roy et al., 2007, López-Arias et al., 2011, Bang et al., 2011, Ofstad et al., 2011, Claridge-Chang et al., 2009, Sadaf et al., 2012, Prieto-Godino et al., 2012, Schneider et al., 2012, Tayler et al., 2012, Tayler et al., 2012)
Tagged with	T:Avic\GFP^EGFP (Baines et al., 2001, Hardie et al., 2001, Malpel et al., 2002, Nitabach et al., 2002, Hardie et al., 2002, Kueppers et al., 2003, Hardie et al., 2004, Tsai et al., 2004, Larsson et al., 2004, Leung and Waddell, 2004, Olofsson and Page, 2005, Luan et al., 2006, Nitabach et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective, with Scer\GAL4^Tdc2.PC (Dankert et al., 2009) behavior defective \| RU486 conditional, with Scer\GAL4^{Mef2.247.Switch} (Joiner et al., 2006) chemical resistant, with Scer\GAL4^P2.4.Pdf (Tsai et al., 2004) chemosensitive behavior defective, with Scer\GAL4^Tdc2.PC (Schneider et al., 2012) chemosensitive behavior defective \| adult stage, with Scer\GAL4^4.64 (King et al., 2011) chemosensitive behavior defective \| adult stage, with Scer\GAL4^10.229 (King et al., 2011) chemosensitive behavior defective \| adult stage, with Scer\GAL4^17d (King et al., 2011) chemosensitive behavior defective \| adult stage, with Scer\GAL4^Tdc2.PC (Hardie et al., 2007) female sterile, with Scer\GAL4^Ilp7.PY (Yang et al., 2008) female sterile, with Scer\GAL4^Tdc2.PC (Hardie et al., 2007) flight defective, with Scer\GAL4^Trh.PB (Sadaf et al., 2012) lethal, with Scer\GAL4^DAT.PL (Bang et al., 2011) lethal, with Scer\GAL4^elav-C155 (Nitabach et al., 2002) lethal, with Scer\GAL4^GMR.PF (Malpel et al., 2002) lethal, with Scer\GAL4^ple.PF (Bang et al., 2011) lethal \| larval stage, with Scer\GAL4^dimm-929 (Luan et al., 2006) lethal \| larval stage, with Scer\GAL4^dimm-929, Scer\GAL80^Ccap.PL (Luan et al., 2006) locomotor behavior defective \| adult stage, with Scer\GAL4^238Y (King et al., 2011) locomotor behavior defective \| adult stage, with Scer\GAL4^Tdc2.PC (Hardie et al., 2007) locomotor behavior defective \| adult stage \| heat sensitive, with Scer\GAL4^Ddc.HL9 (Claridge-Chang et al., 2009) locomotor behavior defective \| adult stage \| heat sensitive, with Scer\GAL4^ple.PF (Claridge-Chang et al., 2009) locomotor behavior defective \| third instar larval stage, with Scer\GAL4^dimm-929 (Suster et al., 2003) locomotor rhythm defective, with Scer\GAL4^P2.4.Pdf (Nitabach et al., 2002, Nitabach et al., 2006) locomotor rhythm defective \| RU486 conditional, with Scer\GAL4^Pdf.Switch1a (Depetris-Chauvin et al., 2011) male sterile, with Scer\GAL4^Crz.391 (Tayler et al., 2012) male sterile, with Scer\GAL4^CrzR.3.5 (Tayler et al., 2012) male sterile, with Scer\GAL4^Trh.1.0 (Tayler et al., 2012) mating defective, with Scer\GAL4^Crz.391 (Tayler et al., 2012) memory defective \| adult stage \| heat sensitive, with Scer\GAL4^ple.PF (Claridge-Chang et al., 2009) neuroanatomy defective, with Scer\GAL4^GMR.PF (Malpel et al., 2002) neuroanatomy defective, with Scer\GAL4^αTub84B.PP (Roy et al., 2007) neuroanatomy defective \| first instar larval stage, with Scer\GAL4^Lim3b (Prieto-Godino et al., 2012) neuroanatomy defective \| larval stage, with Scer\GAL4^Orco.PU (Prieto-Godino et al., 2012) neuroanatomy defective \| RU486 conditional, with Scer\GAL4^Pdf.Switch1a (Depetris-Chauvin et al., 2011) neuroanatomy defective \| third instar larval stage (Koon et al., 2011) neuroanatomy defective \| third instar larval stage, with Scer\GAL4^Mhc.PW (Paradis et al., 2001) neurophysiology defective, with Scer\GAL4^eve.RRC (Baines et al., 2001) neurophysiology defective, with Scer\GAL4^Trh.PB (Sadaf et al., 2012) neurophysiology defective, with Scer\GAL4^unspecified (Yuan et al., 2011) neurophysiology defective \| larval stage, with Scer\GAL4^Orco.PU (Prieto-Godino et al., 2012) neurophysiology defective \| RU486 conditional, with Scer\GAL4^Pdf.Switch1a (Depetris-Chauvin et al., 2011) neurophysiology defective \| third instar larval stage, with Scer\GAL4^Mhc.PW (Paradis et al., 2001) paralytic \| embryonic stage, with Scer\GAL4^elav-C155 (Olofsson and Page, 2005) partially lethal - majority die \| pupal stage, with Scer\GAL4^burs.PP (Peabody et al., 2008) partially lethal - majority die \| pupal stage \| heat sensitive, with Scer\GAL4^burs.PP, Scer\GAL80^ts.αTub84B (Peabody et al., 2008) phototaxis defective, with Scer\GAL4^cry.PZ (Keene et al., 2011) phototaxis defective, with Scer\GAL4^tim.PE (Keene et al., 2011) phototaxis defective, with Scer\GAL4^tim.PE, Scer\GAL80^cry.PS (Keene et al., 2011) phototaxis defective, with Scer\GAL4^tim.PE, Scer\GAL80^Pdf.PS (Keene et al., 2011) short lived, with Scer\GAL4^Lk.2.2 (López-Arias et al., 2011) smell perception defective, with Scer\GAL4^ey-OK107 (Ofstad et al., 2011) smell perception defective, with Scer\GAL4^GMR9A11 (Ofstad et al., 2011) smell perception defective, with Scer\GAL4^GMR10B08 (Ofstad et al., 2011) smell perception defective, with Scer\GAL4^GMR67B04 (Ofstad et al., 2011) starvation stress response defective, with Scer\GAL4^Ast.2.1 (Hergarden et al., 2012) stress response defective, with Scer\GAL4^Lk.2.2 (López-Arias et al., 2011) taste perception defective, with Scer\GAL4^E409 (Fischler et al., 2007) taste perception defective, with Scer\GAL4^Lk.2.2 (López-Arias et al., 2011) thermotaxis behavior defective \| adult stage \| heat sensitive, with Scer\GAL4^DAT.PL, Scer\GAL80^ts.αTub84B (Bang et al., 2011) thermotaxis behavior defective \| adult stage \| heat sensitive, with Scer\GAL4^ple.PF, Scer\GAL80^ts.αTub84B (Bang et al., 2011) visible \| heat sensitive, with Scer\GAL4^burs.PP, Scer\GAL80^ts.αTub84B (Peabody et al., 2008) visual behavior defective, with Scer\GAL4^GMR15B07 (Ofstad et al., 2011) visual behavior defective, with Scer\GAL4^GMR28D01 (Ofstad et al., 2011)
Phenotype Manifest In
	A1-7 dorsal acute muscle 1, with Scer\GAL4^eve.RRC (Baines et al., 2001) aCC neuron, with Scer\GAL4^eve.RRC (Baines et al., 2001) Bolwig nerve, with Scer\GAL4^GMR.PF (Malpel et al., 2002) CSD interneuron, with Scer\GAL4^αTub84B.PP (Roy et al., 2007) embryonic/larval muscle system, with Scer\GAL4^Mhc.PW (Paradis et al., 2001) embryonic/larval neuromuscular junction \| first instar larval stage \| heat sensitive, with Scer\GAL4^Tdc2.PC, Scer\GAL80^ts.αTub84B (Koon et al., 2011) embryonic/larval neuromuscular junction \| third instar larval stage, with Scer\GAL4^Tdc2.PC (Koon et al., 2011) embryonic/larval olfactory receptor neuron, with Scer\GAL4^Orco.PU (Prieto-Godino et al., 2012) embryonic/larval olfactory receptor neuron \| first instar larval stage, with Scer\GAL4^Lim3b (Prieto-Godino et al., 2012) larval head, with Scer\GAL4^Ccap.PP (Luan et al., 2006) larval LN period neuron, with Scer\GAL4^GMR.PF (Malpel et al., 2002) leg, with Scer\GAL4^Ccap.PP (Luan et al., 2006) l-LNv neuron \| RU486 conditional, with Scer\GAL4^Pdf.Switch1a (Depetris-Chauvin et al., 2011) LNv neuron \| third instar larval stage, with Scer\GAL4^unspecified (Yuan et al., 2011) Malpighian tubule, with Scer\GAL4^Lk.2.2 (López-Arias et al., 2011) multidendritic neuron, with Scer\GAL4^Tdc2.PC (Koon et al., 2011) multidendritic neuron \| heat sensitive, with Scer\GAL4^Tdc2.PC, Scer\GAL80^ts.αTub84B (Koon et al., 2011) NMJ bouton, with Scer\GAL4^Mhc.PW (Paradis et al., 2001) photoreceptor cell, with Scer\GAL4^ninaE.PT (Hardie et al., 2001) proboscis, with Scer\GAL4^Lk.2.2 (López-Arias et al., 2011) projection neuron \| larval stage, with Scer\GAL4^Orco.PU (Prieto-Godino et al., 2012) protocerebrum \| RU486 conditional, with Scer\GAL4^Pdf.Switch1a (Depetris-Chauvin et al., 2011) type I bouton, with Scer\GAL4^Tdc2.PC (Koon et al., 2011) type II bouton, with Scer\GAL4^Tdc2.PC (Koon et al., 2011) type II bouton \| first instar larval stage \| heat sensitive, with Scer\GAL4^Tdc2.PC, Scer\GAL80^ts.αTub84B (Koon et al., 2011) ventral nerve cord \| embryonic stage, with Scer\GAL4^elav-C155 (Olofsson and Page, 2005) wing, with Scer\GAL4^Ccap.PP (Luan et al., 2006) wing \| adult stage A1, with Scer\GAL4^burs.PP (Peabody et al., 2008) wing \| adult stage A1 \| heat sensitive, with Scer\GAL4^burs.PP, Scer\GAL80^ts.αTub84B (Peabody et al., 2008)
Detailed Description
	Statement Reference Expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Ast.2.1] (using the temperature sensitive Scer\GAL80[ts.αTub84B] allele to restrict expression to the adult stage) significantly increases the fraction of flies that feed within 1 hour following 24 hours of starvation (with a food source of 100mM sucrose plus 500mM NaCl). (Hergarden et al., 2012) The gross morphology of the projection neurons appears normal in larvae expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Orco.PU]. However, there is a small but significant increase in projection neuron dendrite occupancy within the antennal lobe compared to controls. Action potentials can not be recorded from the larval olfactory sensory neurons. First instar larvae expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Lim3b] have a significantly higher proportion of olfactory sensory neurons (OSNs) with immature-like morphologies (with broad axonal terminals and multiple filopodia that are more characteristic of earlier developmental stages) than control larvae. The OSNs occupy a larger percentage of the antennal lobe synaptic volume than controls. (Prieto-Godino et al., 2012) Flies expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Trh.PB] show reduced flight ability in a cylinder drop test. Air-puff stimulated flight responses of the dorsal longitudinal indirect flight muscle (DLM) of tethered flies expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Trh.PB] are different to wild type. In 12/30 cases the electrophysiological response was reduced to less than 5 seconds, in 9/30 cases an intermittent response was seen, and 9/30 flies showed sustained electrophysiological responses similar to controls. (Sadaf et al., 2012) Flies expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Tdc2.PC] (the temperature-sensitive Scer\GAL80[ts.αTub84B] allele is used to prevent expression during development) fail to show the normal preference for ethanol in an olfactory choice assay. (Schneider et al., 2012) Expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Crz.391] in males results in an increase in the duration of copulation by five-fold, and in male infertility. Courtship index of the males is normal. Males expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Crz.391] fail to transfer either sperm or seminal fluid to females during copulation. Males expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of either Scer\GAL4[CrzR.3.5] or Scer\GAL4[Trh.1.0] are sterile and fail to transfer either sperm or seminal fluid to females during copulation. Copulation duration is normal. (Tayler et al., 2012) Females expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Crz.391] mate normally within 1 hour, show normal copulation durations and are fertile. Flies in which expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Crz.391] is restricted to the adult stage (using the temperature sensitive Scer\GAL80[ts.αTub84B] allele) show increased copulation duration and reduced male fertility compared to controls. Males expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Crz.2fa] show normal copulation durations. Males expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Crz.391] and decapitated during mating show an increased duration of copulation compared to controls. (Tayler et al., 2012) Flies in which the expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of either Scer\GAL4[DAT.PL] or Scer\GAL4[ple.PF] is limited (using the temperature sensitive Scer\GAL80[ts.αTub84B] allele) to the adult stage for 16 hours prior to assaying show abnormal temperature-preference behaviour; they show a severe loss in cold avoidance, with many of the flies moving towards the 15[o]C area (the lower limit of the assay apparatus). (Bang et al., 2011) Wild-type mated females often excrete fecal deposits which are extremely concentrated, referred to as reproductive oblong deposits (RODs), compared to the fecal deposits of wild-type males and wild-type virgin females. Mated females expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[eve.RN2] show reduced ROD production compared to wild-type females. Expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Lk.2.2] during the adult stage (using the temperature sensitive Scer\GAL80[ts.αTub84B] allele to restrict the timing of expression) results in the mutant flies producing more concentrated fecal deposits of a much smaller size than those produced by control flies. These phenotypes are seen after only 24 hours of expression. After 4 days of expression, the flies become bloated; the abdominal diameter is much larger than that of controls. The average weight of the mutant flies is increased compared to controls, although the average dry weight is comparable in mutant and wild-type flies, indicating that the mutant flies are showing fluid retention. The defecation rate of the mutant flies after 4 days of expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Lk.2.2] is doubled compared to controls. Adults expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Ilp7.PY] show an increased fecal output compared to controls after 24 hours on a low calorie diet, while after 72 hours on the low calorie diet, the defecation rate of mutant and wild-type flies is comparable. Adults expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Ilp2.215-3] show a decreased fecal output on a low calorie diet compared to controls. (Cognigni et al., 2011) In the absence of inducer (RU486), flies expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Pdf.Switch1a] display normal circadian locomotor activity. In the presence of RU486, flies expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Pdf.Switch1a] display impaired circadian locomotor rhythm. The circadian locomotor rhythm defects resulting from short-term Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP]-overexpression are reversible. Long term Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP]-overexpression (from early development into adulthood) triggers irreversible effects on locomotor behaviour. Overexpression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Pdf.Switch1a] completely abolishes neuronal activity without affecting the survival of large LNv neurons. The electrical silencing of LNv neurons is reversible. It depends on the presence of RU486. Brains expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Pdf.Switch1a] display grossly normal axon projections at the dorsal protocerebrum. However, the total number of axonal crosses is reduced, compared with wild-type, displaying a degree of complexity during the subjective day which resembles the night-time configuration of wild-type. (Depetris-Chauvin et al., 2011) Silencing all tim neurons through expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[tim.PE] severely disrupts the fly's capacity for light avoidance. However, expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] in the lateral neurons under the control of Scer\GAL4[P2.4.Pdf] does not affect light avoidance. Expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] in all circadian clock neurons but not the lateral neurons (through the use of Scer\GAL4[tim.PE] and Scer\GAL80[Pdf.PS]) results in reduced light avoidance. Expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[cry.PZ] results in larvae displaying an increased light avoidance phenotype. Expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] in circadian clock neurons (under the control of Scer\GAL4[tim.PE] but not cry-expressing neurons (through expression of Scer\GAL80[cry.PS]) results in a reduced light avoidance phenotype. (Keene et al., 2011) Expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[238Y] (restricted to the adult stages using Scer\GAL80[ts.αTub84B]) results in gross locomotor defects in the absence of ethanol. Expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] during adult stages under the control of either Scer\GAL4[4.64] or Scer\GAL4[10.229] results in flies that exhibit reduced ethanol-induced hyperactivity compared to controls. A reduction in hyperactivity is also seen when Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] is expressed under the control of the α/β core/surface subset specific driver Scer\GAL4[17d]. (King et al., 2011) Expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Tdc2.PC] results in the complete elimination of type II innervation. In 60% of the nerves examined, these axons stalled in the segmental nerve. However, in 31% of cases the axons travel the entire distance from the central nervous system and stall close to the neuromuscular junction without innervating the muscles. Larvae expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] in octopaminergic neurons under the control of Scer\GAL4[Tdc2.PC] and Scer\GAL80[ts.αTub84B], raised at 18[o]C (the restrictive temperature) and then switched to 29[o]C at the third-instar larval stage do not display any abnormality in type II bouton morphology. In contrast, blocking activity from the late second-instar stage results in breaks in type II arbors. This phenotype is most pronounced when activity is blocked from the late first-instar larval stage. Expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] in octopaminergic neurons, under the control of Scer\GAL4[Tdc2.PC], results in the elimination of type II boutons. The absence of type II innervation leads to a substantial reduction in the number of type I boutons. (Koon et al., 2011) Median and maximum lifespan is significantly reduced as a result of expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Lk.2.2]. Many adults expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Lk.2.2] develop small necrosis in the proboscis and Malpighian tubules. Resistance to dry starvation is reduced in flies expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Lk.2.2]. When males expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Lk.2.2] are tested for trehalose sensitivity, they show an impaired gustatory response. Males expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Lk.2.2] show different responses to the odorants isoamyl acetate, ethyl acetate and benzaldehyde than do wild-type flies. Females expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Lk.2.2] show different responses to the odorants isoamyl acetate and ethyl acetate than do wild-type flies. Bitter compounds (such as quinine and berberine) are significantly more strongly avoided by flies expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Lk.2.2] than by wild-type control flies. Flies expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Lk.2.2] respond in the same way as wild-type controls for some other bitter tastes and NaCl. (López-Arias et al., 2011) Silencing the mushroom body intrinsic neurons through expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[GMR9A11], Scer\GAL4[GMR10B08], Scer\GAL4[GMR67B04] or Scer\GAL4[ey-OK107] has no significant effect on the performance of flies in visual place learning. Silencing in the mushroom bodies leads to a total loss of odour learning. Silencing subsets of neurons with projections to the central complex ellipsoid body, through expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[GMR15B07] or Scer\GAL4[GMR28D01] dramatically impairs visual place learning. These flies display normal locomotor, optomotor, thermosensory and visual pattern discrimination behaviours. These flies display no change in odour learning. Silencing a subset of ring neurons through expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[GMR38H02] leaves visual place learning intact. (Ofstad et al., 2011) Ventral lateral LN(v) neurons expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] show reduced or no calcium responses upon light stimulation. (Yuan et al., 2011) Expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Or67d-GAL4-1] blocks the ability of synthetic 11-cis-vaccenyl acetate (cVA) to promote male-male aggression. Expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Or67d-GAL4-1] blocks the ability of synthetic cVA to suppress male mating towards females. Expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Or65a.PF] does not impair the ability of synthetic cVA to promote male-male aggression and to suppress male mating towards females. The ability of the presence of males (these donor males are caged in a meshed compartment so that they can act as a donor of pheromones but they cannot interact physically with the tested males) to enhance aggression in other males is eliminated by expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Or67d-GAL4-1] in the tested males. Males expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Or67d-GAL4-1] do not show increased dispersal in the presence of synthetic cVA, unlike control flies, when several males are placed in a setting to compete for a limited food resource territory. (Wang and Anderson, 2010) Expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[ple.PF] (but not under the control of Scer\GAL4[Ddc.HL9]) at 30[o]C blocks action-contingent averse conditioning. Expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of either Scer\GAL4[ple.PF] or Scer\GAL4[Ddc.HL9] at 30[o]C results in a decrease in basal locomotor activity to less than 25% of baseline. (Claridge-Chang et al., 2009) Flies expressing the inwardly rectifying potassium channel Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] in Tdc2-expressing neurons (i.e under the control of Scer\GAL4[Tdc2.PC]) show significant decreases in lunging, tussling, and wing-threats. There is no significant change in chasing or total distance travelled. (Dankert et al., 2009) Overexpression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] with Scer\GAL4[burs.PP] results in almost all animals failing to expand their wings, and a significant pupal lethality (170 of 371 animals). Flies containing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] and Scer\GAL80[ts.αTub84B], driven by Scer\GAL4[burs.PP], fail to expand their wings at the restrictive temperature of 31[o]C, but expand their wings normally at the permissive temperature of 18[o]C. Almost all of these animals survive pupal development if grown at 18[o]C and then transferred to 31[o]C at the latest stages of development, but fail to expand their wings, in contrast to those raised only at 18[o]C. This phenotype is evident in a substantial number of animals when they are transferred to 31[o]C as late as 8 days after puparium formation. (Peabody et al., 2008) Females expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Tre.8.5.cCa] show a decrease in egg-laying bias against a sucrose containing medium compared to control females. Females expressing Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Ilp7.PY] show no discernible developmental defects but display virtually no ovipositor motor programs and thus are sterile. (Yang et al., 2008) Flies expressing Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^E409 (Scer\GAL80^ts.αTub84B also present in the flies has been inactivated by raising the flies overnight at 30^oC) show reduced preference for soluble CO₂ compared to wild-type controls. Flies expressing Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^E409 (Scer\GAL80^ts.αTub84B also present in the flies has been inactivated by raising the flies overnight at 30^oC) avoid 1% gas-phase CO₂, as do wild-type flies. (Fischler et al., 2007) Expression of Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Tdc2.PC produces sterile female flies which show an egg retention phenotype, similar to that seen in Tdc2^RO54 flies. Adult males which express Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Tdc2.PC show a significant decrease in locomotor activity compared to control lines. These flies are also hypersensitive to cocaine but show similar behavioral sensitization to repeated doses of cocaine to control flies. (Hardie et al., 2007) Targeted expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] driven by Scer\GAL4[αTub84B.PP] results in dramatic alterations in the architecture of the CSD interneuron (CSDn). (Roy et al., 2007) Expression of Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^{Mef2.247.Switch} (in the presence of RU486) causes a significant increase in sleep. (Joiner et al., 2006) Flies expressing Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} (under the control of Scer\GAL4^Ccap.PP) die with head eversion defects and foreshortened wings and legs, with only <1% surviving to adulthood. Expression of Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^dimm-929 is lethal early in larval development. This effect is not blocked by co-expression of Scer\GAL80^Ccap. (Luan et al., 2006) Expression of Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}, under the control of Scer\GAL4^P2.4.Pdf induces behavioural arrhythmicity. (Nitabach et al., 2006) Expression of Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} throughout the CNS, using Scer\GAL4^elav-C155 as a driver, results in paralysis and a significant inhibition of embryonic ventral nerve cord condensation. (Olofsson and Page, 2005) When Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} is driven by Scer\GAL4^P2.4.Pdf mutant animals exhibit a reduced sensitivity to cocaine. (Tsai et al., 2004) Third instar larvae expressing Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^dimm-929 show altered locomotion compared to wild-type larvae; there is a significant reduction in average crawling speed, although there is no change in turning rate. (Suster et al., 2003) Expression of Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PF at 25^oC results in 80 +/- 2% lethality. Expression of Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PF results in defects in the Bolwig's nerve and lateral neurons in third instar larvae. (Malpel et al., 2002) Expression of Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^P2.4.Pdf does not alter the number of adult lateral pacemaker neurons, and they show their normal dorso-medial projection into the central brain. Almost all flies expressing Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^P2.4.Pdf are arrhythmic with respect to free-running locomotor activity. In LD conditions, flies expressing Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^P2.4.Pdf begin to increase their activity about 4 hours before lights-off (wild-type flies begin to increase their activity about 2 hours before lights-off). (Nitabach et al., 2002) Expression of Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} in the aCC using Scer\GAL4^eve.RRC results in the almost total absence of excitatory junctional currents in its target muscle (DA1). Spontaneous release of neurotransmitter however, is unaffected. Frequency of synaptic input to the aCC is unaffected, although sustained currents fail to trigger action potentials. Peak I_Kfast and I_Na in the aCC show no significant differences compared to controls. (Baines et al., 2001) Photoreceptor cells expressing Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^ninaE.PT display a large potassium conductance immediately on establishing the whole-cell configuration in vitro, but light responses are unaffected. The conductance (IRK) shows the inwardly rectifying properties of Hsap\KCNJ2 and its characteristic voltage-dependent block by external Ca²⁺. Under control conditions, the IRK current is maintained for the life of the recording (up to 20 minutes) as long as ATP is included in the pipette. In the absence of ATP the current gradually decays. Following stimulation by light under control conditions, the IRK current shows at most a slight suppression. In the presence of La³⁺, the IRK current is profoundly suppressed in response to light stimulation. Following suppression, the IRK recovers to preillumination levels over a period of approximately 1 minute. The light-induced suppression of the IRK current in the presence of La³⁺ is reduced when Li⁺ is present during the inactivation stimulus. (Hardie et al., 2001) Larvae expressing Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Mhc.PW do not show a dramatic alteration in synapse morphology at the neuromuscular junction, although there is a small but statistically significant decrease both in muscle size and bouton number at synapses expressing Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Mhc.PW. There is no significant change in bouton size at these synapses compared to wild type. Expression of Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Mhc.PW alters the passive membrane properties of muscles. The resting membrane potential is hyperpolarised by approximately 15mV, the input resistance is lowered approximately 50-fold and the muscle membrane time constant is ten times faster compared to controls. The average amplitude of spontaneous miniature-release events (mEPSPs) of muscles expressing Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Mhc.PW is decreased approximately 50% compared to controls. Evoked synaptic depolarisations (EPSPs) are approximately 30% larger than those in control animals and depolarise the muscle to the same absolute membrane potential as in the wild type. There is no difference in the average amplitude of the spontaneous miniature synaptic currents (mEPSCs) in larvae expressing Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Mhc.PW compared to controls. The mEPSC frequency is increased by approximately 50% compared to controls. The amplitude of the evoked synaptic current (EPSC) is increased by 34% and there is a significant increase in quantal content compared to wild type. (Paradis et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^P2.4.Pdf has locomotor rhythm defective phenotype, suppressible by Bhal\NaChBac^Scer\UAS.cNa, Scer\GAL4^P2.4.Pdf (Nitabach et al., 2006)
NOT suppressed by
Statement Reference Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^P2.4.Pdf has locomotor rhythm defective phenotype, non-suppressible by Ork1^{Δ-NC.Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^P2.4.Pdf (Nitabach et al., 2006)
Suppressor of
Statement Reference Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^Ast.2.1 is a suppressor of starvation stress response defective phenotype of Bhal\NaChBac^Scer\UAS.cNa, Scer\GAL4^Ast.2.1 (Hergarden et al., 2012) Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^P2.4.Pdf is a suppressor of locomotor rhythm defective phenotype of Bhal\NaChBac^Scer\UAS.cNa, Scer\GAL4^P2.4.Pdf (Nitabach et al., 2006)
Phenotype Manifest In
Other
Statement Reference Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^ninaE.PT, rdgB⁹ has photoreceptor cell phenotype (Hardie et al., 2001, Hardie et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Co-expression of Hsap\KCNJ2[Scer\UAS.T:Avic\GFP-EGFP] rescues the suppression of starvation-induced feeding caused by expression of Bhal\NaChBac[Scer\UAS.cNa] under the control of Scer\GAL4[Ast.2.1]. (Hergarden et al., 2012) Expression of Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} with Ork1^{Δ-NC.Scer\UAS.T:Avic\GFP-EGFP}, under the control of Scer\GAL4^P2.4.Pdf induces behavioural arrhythmicity in over 60% of flies. Expression of Bhal\NaChBac^Scer\UAS.cNa with Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}, under the control of Scer\GAL4^P2.4.Pdf, suppresses the arrhythmicity induced by Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}, with only approximately 12-20% of flies exhibiting behavioural arrhythmicity and most flies exhibiting single rhythms, although these tend to be weaker than those exhibited in wild-type flies. This coexpression decreases the proportion of flies that exhibit complex rhythmicity induced by Bhal\NaChBac^Scer\UAS.cNa expression. (Nitabach et al., 2006) After a light stimulus just sufficient to induce decay, the photoreceptor cell IRK current is suppressed in rdgB⁹ flies expressing Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^ninaE.PT (as occurs in a rdgB⁺ background), but the current recovers at most about 50% over a period of several minutes (in flies expressing Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^ninaE.PT in a rdgB⁺ background full recovery with a half-time of about 30 seconds is seen). (Hardie et al., 2001)
Complementation & Rescue Data
Comments
Stocks ( 4 )
Bloomington	6595 w^; P{UAS-Hsap\KCNJ2.EGFP}7 6596 w^; P{UAS-Hsap\KCNJ2.EGFP}1
Kyoto	108845 w^; P{UAS-Hsap\KCNJ2.EGFP}7 108846 w^; P{UAS-Hsap\KCNJ2.EGFP}1
Notes on Origin
Discoverer

Comments
	Shows normal GABA staining in stage 17 nerve cord, when expression is driven by Scer\GAL4^elav-C155. (Kueppers et al., 2003)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} Kir2.1^WT (Hardie et al., 2004) UAS-K^ir2.1 (Leung and Waddell, 2004)
Name Synonym
Secondary FlyBase IDs

References ( 40 )

Generate a list of	All references relating to this allele ( 40 )

List References by type	Research paper ( 38 ) Supplementary material ( 1 ) Review ( 1 )
Recent research papers ( 14 )
	Hergarden et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(10): 3967--3972 Allatostatin-A neurons inhibit feeding behavior in adult Drosophila. [FBrf0217661] Prieto-Godino et al., 2012, PLoS Biol. 10(10): e1001400 Embryonic origin of olfactory circuitry in Drosophila: contact and activity-mediated interactions pattern connectivity in the antennal lobe. [FBrf0219665] Sadaf et al., 2012, PLoS ONE 7(9): e46405 Synaptic Activity in Serotonergic Neurons Is Required for Air-Puff Stimulated Flight in Drosophila melanogaster. [FBrf0219558] Schneider et al., 2012, PLoS ONE 7(12): e52007 Neuronal basis of innate olfactory attraction to ethanol in Drosophila. [FBrf0220349] Tayler et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(50): 20697--20702 A neuropeptide circuit that coordinates sperm transfer and copulation duration in Drosophila. [FBrf0220217] Bang et al., 2011, PLoS Genet. 7(3): e1001346 Dopamine signalling in mushroom bodies regulates temperature-preference behaviour in Drosophila. [FBrf0213392] Cognigni et al., 2011, Cell Metab. 13(1): 92--104 Enteric neurons and systemic signals couple nutritional and reproductive status with intestinal homeostasis. [FBrf0212594] Depetris-Chauvin et al., 2011, Curr. Biol. 21(21): 1783--1793 Adult-specific electrical silencing of pacemaker neurons uncouples molecular clock from circadian outputs. [FBrf0216659] Keene et al., 2011, J. Neurosci. 31(17): 6527--6534 Distinct visual pathways mediate Drosophila larval light avoidance and circadian clock entrainment. [FBrf0213587] King et al., 2011, J. Neurosci. 31(3): 1139--1148 Drosophila tao Controls Mushroom Body Development and Ethanol-Stimulated Behavior through par-1. [FBrf0212818] Koon et al., 2011, Nat. Neurosci. 14(2): 190--199 Autoregulatory and paracrine control of synaptic and behavioral plasticity by octopaminergic signaling. [FBrf0212895] López-Arias et al., 2011, Peptides 32(3): 545--552 Blockade of the release of the neuropeptide leucokinin to determine its possible functions in fly behavior: Chemoreception assays. [FBrf0213007] Ofstad et al., 2011, Nature 474(7350): 204--207 Visual place learning in Drosophila melanogaster. [FBrf0213884] Yuan et al., 2011, Science 333(6048): 1458--1462 Light-induced structural and functional plasticity in Drosophila larval visual system. [FBrf0215276] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...

Allele Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP

Allele Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}