Allele Dmel\Eip93F^Scer\UAS.cLa

General Information
Symbol	Dmel\Eip93F^Scer\UAS.cLa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Lee	FlyBase ID	FBal0123173
Feature type	allele	Associated gene	Dmel\Eip93F

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Lee and Baehrecke, 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Sequencing indicates that the initial AUG codon is mutated in this line. (Mou et al., 2012) Scer\UAS sequences drive expression of the Eip93F full length open reading frame. (Lee et al., 2000)
Carried in construct	P{UAS-Eip93F.L} (Lee and Baehrecke, 2001, Lee et al., 2000, Mou et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell death, with Scer\GAL4^hs.PB (Lee and Baehrecke, 2001) increased cell death, with Scer\GAL4^vg.PM (Lee et al., 2000) increased cell death \| pair rule expression pattern, with Scer\GAL4^h-1J3 (Lee and Baehrecke, 2001) lethal, with Scer\GAL4^hs.PB (Lee and Baehrecke, 2001) lethal \| pair rule expression pattern, with Scer\GAL4^h-1J3 (Lee and Baehrecke, 2001) lethal \| pupal stage, with Scer\GAL4^vg.PM (Lee et al., 2000)
Phenotype Manifest In
	embryo, with Scer\GAL4^h-1J3 (Lee and Baehrecke, 2001) embryo, with Scer\GAL4^hs.PB (Lee and Baehrecke, 2001) wing disc, with Scer\GAL4^vg.PM (Lee et al., 2000)
Detailed Description
	Statement Reference When embryos are heat shocked to induce Scer\GAL4^hs.PB during embryogenesis widespread cell death occurs. When expression is driven by Scer\GAL4^h-1J3, cell death occurs in stripes in the embryos, which subsequently die at the end of embryogenesis with head defects and fusion/deletion of alternate denticle belts. (Lee and Baehrecke, 2001) When Eip93F^Scer\UAS.cLa is driven by Scer\GAL4^vg.PM, all animals die at the start of pupal development. Large amounts of ectopic cell death is seen in the wing and hinge regions of the wing disc. Wing disc morphogenesis is defective, such that the wing does not elongate and appears to fold back on itself. Animals die soon after head eversion during metamorphosis and exhibit defects in the presumptive adult notum and wing. (Lee et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Eip93F^Scer\UAS.cLa, Scer\GAL4^h-1J3 has increased cell death \| pair rule expression pattern phenotype, suppressible by Df(3L)H99 (Lee and Baehrecke, 2001)
NOT suppressed by
Statement Reference Eip93F^Scer\UAS.cLa, Scer\GAL4^h-1J3 has increased cell death \| pair rule expression pattern phenotype, non-suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^h-1J3 (Lee and Baehrecke, 2001) Eip93F^Scer\UAS.cLa, Scer\GAL4^h-1J3 has lethal \| pair rule expression pattern phenotype, non-suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^h-1J3 (Lee and Baehrecke, 2001) Eip93F^Scer\UAS.cLa, Scer\GAL4^h-1J3 has lethal \| pair rule expression pattern phenotype, non-suppressible by Df(3L)H99 (Lee and Baehrecke, 2001) Eip93F^Scer\UAS.cLa, Scer\GAL4^hs.PB has increased cell death phenotype, non-suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^hs.PB (Lee and Baehrecke, 2001) Eip93F^Scer\UAS.cLa, Scer\GAL4^hs.PB has lethal phenotype, non-suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^hs.PB (Lee and Baehrecke, 2001)
Phenotype Manifest In
NOT suppressed by
Statement Reference Eip93F^Scer\UAS.cLa, Scer\GAL4^h-1J3 has embryo phenotype, non-suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^h-1J3 (Lee and Baehrecke, 2001) Eip93F^Scer\UAS.cLa, Scer\GAL4^h-1J3 has embryo phenotype, non-suppressible by Df(3L)H99 (Lee and Baehrecke, 2001) Eip93F^Scer\UAS.cLa, Scer\GAL4^hs.PB has embryo phenotype, non-suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^hs.PB (Lee and Baehrecke, 2001)
Additional Comments
Genetic Interactions
Statement Reference Cell death phenotype is not suppressed by removal of rpr, W and grim (i.e. Df(3L)H99), though the remaining cell death does not show the nuclear hallmarks or programmed cell death. (Lee and Baehrecke, 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^fkh.PH/Eip93F^Scer\UAS.cLa rescues Df(3R)93F^x2 (Lee et al., 2000)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Eip93F^Scer\UAS.cLa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Lee (Lee and Baehrecke, 2001)
Secondary FlyBase IDs

References ( 3 )
Research paper	Mou et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(8): 2949--2954 Control of target gene specificity during metamorphosis by the steroid response gene E93. [FBrf0217473] Lee and Baehrecke, 2001, Development 128(8): 1443--1455 Steroid regulation of autophagic programmed cell death during development. [FBrf0134571] Lee et al., 2000, Mol. Cell 6(2): 433--443 E93 directs steroid-triggered programmed cell death in Drosophila. [FBrf0129927]

Allele Dmel\Eip93FScer\UAS.cLa

Allele Dmel\Eip93F^Scer\UAS.cLa