Allele Dmel\Atg2^EP3697

General Information
Symbol	Dmel\Atg2^EP3697	Species	D. melanogaster
Name		FlyBase ID	FBal0123243
Feature type	allele	Associated gene	Dmel\Atg2

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Gene Disruption Project members, 2001-, Kraut et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3L:2,774,375..2,774,375 (Kraut et al., 2001, Gene Disruption Project members, 2001-, Scott et al., 2004, Tian et al., 2011, Shen and Ganetzky, 2009, Pircs et al., 2012)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	P{EP}Atg2^EP3697 (Kraut et al., 2001, Gene Disruption Project members, 2001-, Scott et al., 2004, Tian et al., 2011, Shen and Ganetzky, 2009, Pircs et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| larval stage \| recessive, with Scer\GAL4[-]: (Scott et al., 2004) neuroanatomy defective (Shen and Ganetzky, 2009) neuroanatomy defective \| larval stage, with Scer\GAL4^elav-C155 (Kraut et al., 2001)
Phenotype Manifest In
	anterior fascicle & synapse, with Scer\GAL4^elav-C155 (Kraut et al., 2001) embryonic/larval fat body \| third instar larval stage (with Df(3L)BSC119) (Pircs et al., 2012) embryonic/larval midgut \| P-stage (with Df(3L)Exel6091) (Denton et al., 2009) intersegmental nerve, with Scer\GAL4^elav-C155 (Kraut et al., 2001) neuromuscular junction (Shen and Ganetzky, 2009) NMJ bouton (Shen and Ganetzky, 2009)
Detailed Description
	Statement Reference Atg2[EP3697]/Df(3L)BSC119 mutants display basal autophagy defects in larval fat body cells. (Pircs et al., 2012) Atg2[EP3697] heterozygous neuromuscular junctions exhibit wild-type bouton levels. (Tian et al., 2011) Atg2[EP3697]/Df(3L)Exel6091 animals analysed at +4 hours relative to puparium formation show a significant delay in midgut histolysis compared to controls. At +12 hours relative to puparium formation, larval midgut contraction is dramatically delayed in the mutants compared to controls. (Denton et al., 2009) Atg2[EP3697] mutants exhibit neuromuscular junction undergrowth but do not exhibit defects in axonal transport. (Shen and Ganetzky, 2009) Mutants show disruption of autophagy. (Scott et al., 2004) Mutant larvae expressing Atg2^EP3697 under the control of Scer\GAL4^elav-C155 have reduced or abnormal synapses. Mutant larvae expressing Atg2^EP3697 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects. (Kraut et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Atg2^EP3697 is an enhancer of decreased cell growth phenotype of Scer\GAL4^elav-C155, hiw^Scer\UAS.fl (Shen and Ganetzky, 2009) Atg2^EP3697 is an enhancer of neuroanatomy defective phenotype of Scer\GAL4^elav-C155, hiw^Scer\UAS.fl (Shen and Ganetzky, 2009)
Suppressor of
Statement Reference Atg2^EP3697, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, bchs^EP2299 (Simonsen et al., 2007)
Phenotype Manifest In
Enhancer of
Statement Reference Atg2^EP3697 is an enhancer of neuromuscular junction phenotype of Scer\GAL4^elav-C155, hiw^Scer\UAS.fl (Shen and Ganetzky, 2009)
Suppressor of
Statement Reference Atg2[+]/Atg2^EP3697 is a suppressor of NMJ bouton phenotype of Rae1^EX28 (Tian et al., 2011) Atg2[+]/Atg2^EP3697 is a suppressor of synapse phenotype of Rae1^EX28 (Tian et al., 2011) Atg2^EP3697, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, bchs^EP2299 (Simonsen et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference An Atg2[EP3697] heterozygous background dominantly suppresses the increase in bouton number seen in Rae1[EX28] homozygous mutant neuromuscular junction. (Tian et al., 2011) The modest neuromuscular undergrowth seen upon expression of hiw[Scer\UAS.fl] under the control of Scer\GAL4[elav-C155] is enhanced in a Atg2[EP3697] heterozygous background. (Shen and Ganetzky, 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	17156 w¹¹¹⁸; P{EP}Atg2^EP3697/TM6B, Tb¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Atg2^EP3697 (Tian et al., 2011, Denton et al., 2009) atg2^EP3697 (Simonsen et al., 2007, Shen and Ganetzky, 2009) EP3697^EP3697 (Kraut et al., 2001, )
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Pircs et al., 2012, PLoS ONE 7(8): e44214 Advantages and Limitations of Different p62-Based Assays for Estimating Autophagic Activity in Drosophila. [FBrf0219364] Tian et al., 2011, Nat. Neurosci. 14(10): 1267--1275 Drosophila Rae1 controls the abundance of the ubiquitin ligase Highwire in post-mitotic neurons. [FBrf0216253] Denton et al., 2009, Curr. Biol. 19(20): 1741--1746 Autophagy, not apoptosis, is essential for midgut cell death in Drosophila. [FBrf0209243] Shen and Ganetzky, 2009, J. Cell Biol. 187(1): 71--79 Autophagy promotes synapse development in Drosophila. [FBrf0208921] Simonsen et al., 2007, Genetics 176(2): 1283--1297 Genetic modifiers of the Drosophila blue cheese gene link defects in lysosomal transport with decreased life span and altered ubiquitinated-protein profiles. [FBrf0201586]
Supplementary material	Scott et al., 2004, Dev. Cell 7(2): Supplementary material. [FBrf0183440] Kraut et al., 2001, Curr. Biol. 11(6): A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135968]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]

Allele Dmel\Atg2EP3697

Allele Dmel\Atg2^EP3697