FlyBase Allele Report: Dmel\garz[EP2028]

General Information

Symbol

Dmel\garz^EP2028

Species

D. melanogaster

Name

FlyBase ID

FBal0123295

Feature type

allele

Associated gene

Dmel\garz

Associated Insertion(s)

P{EP}garz^EP2028

Carried in Construct

Also Known As

EP(2)2028

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Kraut et al., 2001, Kraut et al., 2001)

Progenitor genotype

Associated Insertion(s)

P{EP}garz^EP2028

Cytology

Description

Insertion 298bp upstream of the annotated garz transcription start site.

(Armbruster and Luschnig, 2012)

The orientation of the P{EP} element is the same as the direction of garz transcription.

(Shulman and Feany, 2003)

The P{EP} element is inserted 295 nucleotides 5' to the predicted ATG start codon.

(Kraut et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:12,326,376..12,326,376 [+]

(Armbruster and Luschnig, 2012, Wang et al., 2012, Maybeck and Röper, 2009, Shulman and Feany, 2003, Gene Disruption Project members, 2001-, Kraut et al., 2001, Kraut et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Shulman and Feany, 2003)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior fascicle & synapse, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

anterior fascicle & synapse | supernumerary, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

embryonic/larval salivary gland, with Scer\GAL4^fkh.PH

(Maybeck and Röper, 2009)

eye, with Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Raghu et al., 2009)

larval intersegmental nerve, with Scer\GAL4^elav-C155

(Kraut et al., 2001, Kraut et al., 2001)

rhabdomere, with Scer\GAL4^GMR.PF

(Raghu et al., 2009)

Detailed Description

Statement

Reference

Lethal phase analysis of garz^EP2028 homozygous mutants reveals early first instar larval lethality with a very low number of individuals surviving until the end of larval development.

(Wang et al., 2012)

Expression via Scer\GAL4^fkh.PH results in a weakly penetrant but severe positioning ('hook') phenotype in the embryonic salivary gland.

(Maybeck and Röper, 2009)

Flies expressing garz^EP2028 under the control of Scer\GAL4^GMR.PF in the dark show normal rhabdomere development.

Flies expressing garz^EP2028 under the control of Scer\GAL4^GMR.PF and grown in bright light show some defects in rhabdomere structure and mild accumulation of endomembranes in the cell body of the photoreceptors.

(Raghu et al., 2009)

Expression of garz^EP2028 under the control of Scer\GAL4^GMR.PF results in a very mild rough eye phenotype.

(Shulman and Feany, 2003)

Mutant larvae expressing garz^EP2028 under the control of Scer\GAL4^elav-C155 have excessive or ectopic synapses. Mutant larvae expressing garz^EP2028 under the control of Scer\GAL4^elav-C155 have reduced or abnormal synapses.

(Kraut et al., 2001)

In the ventral muscle region of mutant larvae expressing garz^EP2028 under the control of Scer\GAL4^elav-C155 ISN pathfinding defects can be seen. ISNb leaves the ISN at an abnormal point. It generates an abnormal axonal branch that splits into synaptic branches on incorrect muscles.

(Kraut et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

garz^EP2028, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

NOT Enhancer of

Statement

Reference

garz^EP2028, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

NOT Suppressor of

Statement

Reference

garz^EP2028, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, garz^EP2028 has rhabdomere phenotype, enhanceable by Cds¹

(Raghu et al., 2009)

Enhancer of

Statement

Reference

garz^EP2028/Scer\GAL4^GMR.PF is an enhancer of rhabdomere phenotype of Cds¹

(Raghu et al., 2009)

garz^EP2028, Scer\GAL4^GMR.PF is an enhancer of rhabdomere phenotype of Pld^UAS.cRa, Scer\GAL4^GMR.PF

(Raghu et al., 2009)

garz^EP2028, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

garz^EP2028, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

NOT Enhancer of

Statement

Reference

garz^EP2028, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

NOT Suppressor of

Statement

Reference

garz^EP2028, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of garz^EP2028 strongly enhances the retina phenotype caused by expression of Pld^Scer\UAS.cRa under the control of Scer\GAL4^GMR.PF; only one or two poorly developed rhabdomeres are seen.

Expression of garz^EP2028 under the control of Scer\GAL4^GMR.PF in a CdsA¹ background under bright light illumination results in significantly worse defects in rhabdomere size and structure compared to CdsA¹ single mutants of flies expressing garz^EP2028 under the control of Scer\GAL4^GMR.PF in a wild-type background. There is no change in the accumulation of membranes in the cell body of the photoreceptors in the double mutant flies.

(Raghu et al., 2009)

Xenogenetic Interactions

Statement

Reference

The rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by garz^EP2028.

(Shulman and Feany, 2003)

Complementation and Rescue Data

Fails to complement

garz^S4-50

(Wang et al., 2012)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

17017

w¹¹¹⁸; P{EP}garz^EP2028/CyO

Kyoto

114444

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}42D P{EP}garz^EP2028 /CyO y⁺

Notes on Origin

Discoverer

Comments

Precise excision of the P{EP} element reverts the modifying effect of garz^EP2028 on the rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

(Shulman and Feany, 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG8487^EP2028

(Kraut et al., 2001, Kraut et al., 2001)

garz^EP2028

(Armbruster and Luschnig, 2012)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

Open Close