Allele Dmel\y^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\y^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0123543
Feature type	allele	Associated gene	Dmel\y

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Piccin et al., 2001) in vitro construct - RNAi (Piccin et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of an inverted repeat consisting of y coding sequences in sense orientation followed by a 330bp fragment of Avic\GFP followed by y coding sequences in the antisense orientation. (Piccin et al., 2001)
Carried in construct	P{UAS-y.IR} (Piccin et al., 2001, Fujii et al., 2008, Kupsco et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	body color defective, with Scer\GAL4^Act5C.PI (Piccin et al., 2001) body color defective, with Scer\GAL4^da.G32 (Piccin et al., 2001, Kupsco et al., 2006) body color defective, with Scer\GAL4^hs.PB (Piccin et al., 2001)
Phenotype Manifest In
	adult cuticle, with Scer\GAL4^Act5C.PI (Piccin et al., 2001) adult cuticle, with Scer\GAL4^da.G32 (Piccin et al., 2001) adult cuticle, with Scer\GAL4^hs.PB (Piccin et al., 2001) adult thorax, with Scer\GAL4^da.G32 (Kupsco et al., 2006) macrochaeta, with Scer\GAL4^Act5C.PI (Piccin et al., 2001) macrochaeta, with Scer\GAL4^hs.PB (Piccin et al., 2001) wing, with Scer\GAL4^Act5C.PI (Piccin et al., 2001) wing, with Scer\GAL4^da.G32 (Piccin et al., 2001) wing, with Scer\GAL4^hs.PB (Piccin et al., 2001)
Detailed Description
	Statement Reference Flies expressing y[dsRNA.Scer\UAS] under the control of Scer\GAL4[da.G32] have a paler thorax than wild-type controls. (Kupsco et al., 2006) Body colour: expression of y^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PI results in 100% of animals displaying a y¹-like phenotype, having a y mutant cuticle, wings and bristles. Body colour: expression of y^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 results in a y²-like phenotype in about 37% of flies, with y mutant cuticle and wings but wild-type bristles. A high proportion of animals show an "incomplete y phenotype", having a yellowish cuticle (especially visible on the pale areas of the abdomen and thorax) ranging from almost y-like to almost wild-type. Body colour: expression of y^{dsRNA.Scer\UAS} under the control of Scer\GAL4^tim.PE does not result in a y mutant phenotype. Body colour: expression of y^{dsRNA.Scer\UAS} under the control of Scer\GAL4^hs.PB using a single 1 hour heat pulse at the late third instar/early pupal stage results in about 5-10% of flies have a y¹-like phenotype, and about 30% of flies having yellow-brown bristles but wild-type wings and body. No y silencing is seen when the heat shock occurs during the embryo, first or second larval or older pupal or adult stages. A 1 hour heat pulse per day from the late third instar until hatching results in a stronger y mutant phenotype. (Piccin et al., 2001)
External Data
Linkouts
Interactions
Phenotypic Class
NOT Enhanced by
Statement Reference Scer\GAL4^da.G32, y^{dsRNA.Scer\UAS} has body color defective phenotype, non-enhanceable by Snp^c00465/Snp^c00465 (Kupsco et al., 2006)
Phenotype Manifest In
NOT Enhanced by
Statement Reference Scer\GAL4^da.G32, y^{dsRNA.Scer\UAS} has adult thorax phenotype, non-enhanceable by Snp^c00465/Snp^c00465 (Kupsco et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference Snp[c00465]/Snp[c00465] does not alter the body pigmentation of flies expressing y[dsRNA.Scer\UAS] under the control of Scer\GAL4[da.G32]. (Kupsco et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	y^{dsRNA.Scer\UAS} y^IR.Scer\UAS
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Fujii et al., 2008, Genetics 180(1): 179--190 A male-specific fatty acid omega-hydroxylase, SXE1, is necessary for efficient male mating in Drosophila melanogaster. [FBrf0207358] Kupsco et al., 2006, RNA 12(12): 2103--2117 Genetic and biochemical characterization of Drosophila Snipper: A promiscuous member of the metazoan 3 ' hExo/ERI-1 family of 3 ' to 5 ' exonucleases. [FBrf0191914] Piccin et al., 2001, Nucleic Acids Res. 29(12): E55 Efficient and heritable functional knock-out of an adult phenotype in Drosophila using a GAL4-driven hairpin RNA incorporating a heterologous spacer. [FBrf0137087]

Allele Dmel\ydsRNA.Scer\UAS

Allele Dmel\y^{dsRNA.Scer\UAS}