FlyBase Allele Report: Dmel\wg[wg.3'UTR.UAS]

General Information

Symbol

Dmel\wg^wg.3'UTR.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0123559

Feature type

allele

Associated gene

Dmel\wg

Associated Insertion(s)

Carried in Construct

P{UAS-wg.wg3'UTR}

Also Known As

UAS-wg

Key Links

Transgenic product class

wild_type

(Simmonds et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Simmonds et al., 2001)

Mutagen

in vitro construct

(Simmonds et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-wg.wg3'UTR}

Cytology

Description

UAS regulatory sequences drive expression of the wg open reading frame followed by the wg 3' UTR.

(Simmonds et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^GMR.PF

(Cadigan et al., 2002)

visible, with Scer\GAL4^GMR.PF

(Cadigan et al., 2002)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Cadigan et al., 2002)

eye disc morphogenetic furrow, with Scer\GAL4^dpp.blk1

(Cadigan et al., 2002)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Cadigan et al., 2002)

Detailed Description

Statement

Reference

Flies expressing wg^{wg.3'UTR.Scer\UAS} under the control of Scer\GAL4^GMR.PF have a dramatically reduced eye which lacks bristles. The reduced eye size is not due to a lack of morphogenetic furrow progression. The eyes show a large degree of apoptosis during pupal development. Expression of wg^{wg.3'UTR.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 completely blocks progression of the morphogenetic furrow.

(Cadigan et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, wg^wg.3'UTR.UAS has visible phenotype, suppressible | partially by da^UAS.cGa/Scer\GAL4^GMR.PF

(Cadigan et al., 2002)

Scer\GAL4^GMR.PF, wg^wg.3'UTR.UAS has visible phenotype, suppressible | partially by Scer\GAL4^GMR.PF/sc^UAS.cHa

(Cadigan et al., 2002)

Scer\GAL4^GMR.PF, wg^wg.3'UTR.UAS has visible phenotype, suppressible | partially by pan^ΔN.UAS/Scer\GAL4^GMR.PF

(Cadigan et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, wg^wg.3'UTR.UAS has interommatidial bristle phenotype, suppressible by Scer\GAL4^GMR.PF/sc^UAS.cHa

(Cadigan et al., 2002)

Scer\GAL4^GMR.PF, wg^wg.3'UTR.UAS has interommatidial bristle phenotype, suppressible | partially by pan^ΔN.UAS/Scer\GAL4^GMR.PF

(Cadigan et al., 2002)

Scer\GAL4^dpp.blk1, wg^wg.3'UTR.UAS has eye disc morphogenetic furrow phenotype, suppressible | partially by ato^UAS.cCa/Scer\GAL4^dpp.blk1

(Cadigan et al., 2002)

Scer\GAL4^dpp.blk1, wg^wg.3'UTR.UAS has eye disc morphogenetic furrow phenotype, suppressible | partially by Scer\GAL4^dpp.blk1/da^UAS.cGa

(Cadigan et al., 2002)

Scer\GAL4^dpp.blk1, wg^wg.3'UTR.UAS has eye disc morphogenetic furrow phenotype, suppressible | partially by pan^ΔN.UAS/Scer\GAL4^dpp.blk1

(Cadigan et al., 2002)

Scer\GAL4^dpp.blk1, wg^wg.3'UTR.UAS has eye disc morphogenetic furrow phenotype, suppressible | partially by Scer\GAL4^dpp.blk1/sc^UAS.cHa

(Cadigan et al., 2002)

Scer\GAL4^GMR.PF, wg^wg.3'UTR.UAS has eye phenotype, suppressible | partially by da^UAS.cGa/Scer\GAL4^GMR.PF

(Cadigan et al., 2002)

Scer\GAL4^GMR.PF, wg^wg.3'UTR.UAS has eye phenotype, suppressible | partially by pan^ΔN.UAS/Scer\GAL4^GMR.PF

(Cadigan et al., 2002)

Scer\GAL4^GMR.PF, wg^wg.3'UTR.UAS has eye phenotype, suppressible | partially by Scer\GAL4^GMR.PF/sc^UAS.cHa

(Cadigan et al., 2002)

Scer\GAL4^GMR.PF, wg^wg.3'UTR.UAS has interommatidial bristle phenotype, suppressible by da^UAS.cGa/Scer\GAL4^GMR.PF

(Cadigan et al., 2002)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, wg^wg.3'UTR.UAS has interommatidial bristle phenotype, non-suppressible by ato^UAS.cCa/Scer\GAL4^GMR.PF

(Cadigan et al., 2002)

Scer\GAL4^dpp.blk1, wg^wg.3'UTR.UAS has eye disc morphogenetic furrow phenotype, non-suppressible by hh^UAS.cAa/Scer\GAL4^dpp.blk1

(Cadigan et al., 2002)

Scer\GAL4^dpp.blk1, wg^wg.3'UTR.UAS has eye disc morphogenetic furrow phenotype, non-suppressible by dpp^UAS.cSa/Scer\GAL4^dpp.blk1

(Cadigan et al., 2002)

Suppressor of

Statement

Reference

wg^wg.3'UTR.UAS/Scer\GAL4^prd.RG1 is a suppressor of ventral denticle belt phenotype of Scer\GAL4^prd.RG1, mid^UAS.cBa

(Buescher et al., 2004)

Other

Statement

Reference

Scer\GAL4^dpp.blk1, hh^UAS.cAa, wg^wg.3'UTR.UAS has eye disc morphogenetic furrow phenotype

(Cadigan et al., 2002)

Scer\GAL4^dpp.blk1, hh^UAS.cAa, wg^wg.3'UTR.UAS has eye disc morphogenetic furrow | ectopic phenotype

(Cadigan et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of wg^{wg.3'UTR.Scer\UAS} results in near complete suppression of the fusion of alternating denticle belts seen in larvae expressing mid^Scer\UAS.cBa under the control of Scer\GAL4^prd.RG1.

(Buescher et al., 2004)

Co-expression of pan^ΔN.Scer\UAS partially suppresses the small eye and lack of interommatidial bristle phenotype caused by expression of wg^{wg.3'UTR.Scer\UAS} under the control of Scer\GAL4^GMR.PF. Co-expression of da^Scer\UAS.cGa does not significantly alter the small eye phenotype caused by expression of wg^{wg.3'UTR.Scer\UAS} under the control of Scer\GAL4^GMR.PF, but does fully suppress the loss of interommatidial bristles seen in these flies. Co-expression of sc^Scer\UAS.cHa does not significantly alter the small eye phenotype caused by expression of wg^{wg.3'UTR.Scer\UAS} under the control of Scer\GAL4^GMR.PF, but does fully suppress the loss of interommatidial bristles seen in these flies. Co-expression of ato^Scer\UAS.cCa does not rescue the loss of interommatidial bristles seen in flies expressing wg^{wg.3'UTR.Scer\UAS} under the control of Scer\GAL4^GMR.PF. Co-expression of pan^ΔN.Scer\UAS significantly rescues morphogenetic furrow progression in flies expressing wg^{wg.3'UTR.Scer\UAS} under the control of Scer\GAL4^dpp.blk1. Co-expression of da^Scer\UAS.cGa restores some progression of the morphogenetic furrow in animals expressing wg^{wg.3'UTR.Scer\UAS} under the control of Scer\GAL4^dpp.blk1. Co-expression of dpp^Scer\UAS.cSa does not rescue progression of the morphogenetic furrow in animals expressing wg^{wg.3'UTR.Scer\UAS} under the control of Scer\GAL4^dpp.blk1. Co-expression of ato^Scer\UAS.cCa significantly rescues progression of the morphogenetic furrow in animals expressing wg^{wg.3'UTR.Scer\UAS} under the control of Scer\GAL4^dpp.blk1. Co-expression of sc^Scer\UAS.cHa restores some progression of the morphogenetic furrow in animals expressing wg^{wg.3'UTR.Scer\UAS} under the control of Scer\GAL4^dpp.blk1. Co-expression of wg^{wg.3'UTR.Scer\UAS} and hh^Scer\UAS.cAa under the control of Scer\GAL4^dpp.blk1 does not rescue morphogenetic furrow progression from the posterior edge of the eye disc, but usually results in one (or occasionally more) ectopic morphogenetic furrows initiating in the interior of the eye primordium and progressing concentrically.

(Cadigan et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

wg^wg.3'UTR.UAS/Scer\GAL4^wg.PM partially rescues wg^l-17

(Simmonds et al., 2001)

Comments

wg^{wg.3'UTR.Scer\UAS} substantially rescues the cuticle defects of wg^l-17 embryos when expressed under the control of Scer\GAL4^wg.PM.

(Simmonds et al., 2001)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

wg^{wg.3'UTR.Scer\UAS}

wg^wg.3'UTR.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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