Allele Dmel\sli^GA20

General Information
Symbol	Dmel\sli^GA20	Species	D. melanogaster
Name		FlyBase ID	FBal0123697
Feature type	allele	Associated gene	Dmel\sli
Also Known As	slit^GA20

Map ( GBrowse )
Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Battye et al., 2001)
Mutagen	ethyl methanesulfonate (Battye et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References deletion 2R:11,763,697..11,763,697 comment=A one base deletion leads to early translation termination. evidence=experimental pr_change=L837\|sli-PC,L813\|sli-PB,L813@\|sli-PA reported_pr_change=L?@ (Battye et al., 2001)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: L?@. Deletion of one base in G-Domain results in a frameshift and immediate translation termination. (Battye et al., 2001)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (Battye et al., 2001)
Phenotype Manifest In
	aCC neuron (Mehta and Bhat, 2001) embryonic/larval ganglionic branch (Gallio et al., 2004) ganglion mother cell GMC1-1a (Mehta and Bhat, 2001) pCC neuron (Mehta and Bhat, 2001) RP2 motor neuron (Mehta and Bhat, 2001) RP2 motor neuron \| ectopic (Mehta and Bhat, 2001) RP2sib neuron (Mehta and Bhat, 2001) ventral nerve cord (Battye et al., 2001)
Detailed Description
	Statement Reference In stage 16 sli^GA20 mutant embryos 46% of the ganglionic branches cross the midline and the rest are either stalled outside the ventral nerve cord or misrouted inside it. (Gallio et al., 2004) 1.9% of expected mutant embryos hatch as first instar larvae. The latest observed stage of mutants is L1. Nerve cord length is 90% of embryo length. Midline guidance failure of repellence phenotype, evidenced by midline crossing, is severe. (Battye et al., 2001) The GMC-1 in mutants frequently (about 10%) divide symmetrically to generate two RP2s instead of an RP2 and a sib. The RP2 and aCC pCC and other neurons are occasionally misplaced within a hemisegment of the CNS of 13-hour or older mutant embryos, however in younger embryos these cells are not displaced and do not cross the midline or segmental boundary. (Mehta and Bhat, 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference sli^GA20 has ventral nerve cord phenotype, enhanceable by robo[+]/robo¹ (Battye et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference 23.8% of dMP2 neurons project contralaterally in sli[GA20]/robo[1] transheterozygous embryos. This phenotype is suppressed by expression of sli[Scer\UAS.cKa] under the control of Scer\GAL4[605]. (Hiramoto and Hiromi, 2006) The effects of sli^GA20 and ru^PLLb on migration of ganglionic branch trachea approaching the ventral midline during stage 16 appear to be additive. (Gallio et al., 2004) Percentage of segments with midline crossovers in robo¹/sli^GA20 transheterozygotes is 60%. (Battye et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Protein null. (Battye et al., 2001)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	sli^GA20 (MacMullin and Jacobs, 2006, ) slit^GA20 (Battye et al., 2001, Hiramoto and Hiromi, 2006)
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Hiramoto and Hiromi, 2006, Nat. Neurosci. 9(1): 58--66 ROBO directs axon crossing of segmental boundaries by suppressing responsiveness to relocalized Netrin. [FBrf0191279] MacMullin and Jacobs, 2006, Dev. Biol. 293(1): 154--164 Slit coordinates cardiac morphogenesis in Drosophila. [FBrf0190142] Bhat, 2005, Genetics 170(1): 149--159 Slit-roundabout signaling neutralizes Netrin-Frazzled-mediated attractant cue to specify the lateral positioning of longitudinal axon pathways. [FBrf0187630] Gallio et al., 2004, Development 131(15): 3605--3614 Rhomboid 3 orchestrates Slit-independent repulsion of tracheal branches at the CNS midline. [FBrf0179216] Battye et al., 2001, J. Neurosci. 21(12): 4290--4298 Repellent signaling by slit requires the leucine-rich repeats. [FBrf0136993] Mehta and Bhat, 2001, Development 128(16): 3161--3168 Slit signaling promotes the terminal asymmetric division of neural precursor cells in the Drosophila CNS. [FBrf0139628]

Allele Dmel\sliGA20

Allele Dmel\sli^GA20